Experiment: Control_ECRC101
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisA and hisF overlap by 19 nucleotides hisF and hisIE overlap by 7 nucleotides hisIE and wzzB are separated by 96 nucleotides
MCAODC_20445: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 895,080 to 895,817
hisA
MCAODC_20450: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 895,799 to 896,575
hisF
MCAODC_20455: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 896,569 to 897,180
hisIE
MCAODC_20460: wzzB - LPS O-antigen chain length determinant protein WzzB, at 897,277 to 898,254
wzzB
Position (kb) 896
897
898 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 895.636 kb on + strand, within hisA at 895.637 kb on - strand, within hisA at 895.638 kb on + strand, within hisA at 895.639 kb on - strand, within hisA at 895.747 kb on + strand at 895.851 kb on + strand at 895.851 kb on + strand at 895.984 kb on + strand, within hisF at 896.047 kb on + strand, within hisF at 896.047 kb on + strand, within hisF at 896.133 kb on + strand, within hisF at 896.137 kb on + strand, within hisF at 896.137 kb on + strand, within hisF at 896.170 kb on + strand, within hisF at 896.170 kb on + strand, within hisF at 896.170 kb on + strand, within hisF at 896.183 kb on - strand, within hisF at 896.216 kb on + strand, within hisF at 896.217 kb on - strand, within hisF at 896.232 kb on - strand, within hisF at 896.511 kb on + strand at 896.511 kb on + strand at 896.529 kb on + strand at 896.537 kb on + strand at 896.537 kb on + strand at 896.569 kb on - strand at 896.674 kb on + strand, within hisIE at 896.676 kb on + strand, within hisIE at 896.676 kb on + strand, within hisIE at 896.693 kb on + strand, within hisIE at 896.796 kb on + strand, within hisIE at 896.968 kb on + strand, within hisIE at 896.969 kb on - strand, within hisIE at 896.983 kb on + strand, within hisIE at 897.056 kb on + strand, within hisIE at 897.057 kb on - strand, within hisIE at 897.100 kb on + strand, within hisIE at 897.165 kb on + strand at 897.165 kb on + strand at 897.291 kb on - strand at 897.291 kb on - strand at 897.291 kb on - strand at 897.397 kb on - strand, within wzzB at 897.401 kb on - strand, within wzzB at 897.416 kb on - strand, within wzzB at 897.492 kb on - strand, within wzzB at 897.495 kb on - strand, within wzzB at 897.535 kb on - strand, within wzzB at 897.535 kb on - strand, within wzzB at 897.535 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.639 kb on - strand, within wzzB at 897.767 kb on - strand, within wzzB at 897.804 kb on - strand, within wzzB at 897.849 kb on - strand, within wzzB at 897.849 kb on - strand, within wzzB at 897.880 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.018 kb on - strand, within wzzB at 898.036 kb on - strand, within wzzB
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101 remove 895,636 + hisA MCAODC_20445 0.75 +1.3 895,637 - hisA MCAODC_20445 0.75 +0.5 895,638 + hisA MCAODC_20445 0.76 -1.7 895,639 - hisA MCAODC_20445 0.76 -0.2 895,747 + -0.7 895,851 + -0.1 895,851 + -1.0 895,984 + hisF MCAODC_20450 0.24 +1.1 896,047 + hisF MCAODC_20450 0.32 +0.1 896,047 + hisF MCAODC_20450 0.32 +0.5 896,133 + hisF MCAODC_20450 0.43 -0.4 896,137 + hisF MCAODC_20450 0.44 +0.6 896,137 + hisF MCAODC_20450 0.44 +2.5 896,170 + hisF MCAODC_20450 0.48 -1.6 896,170 + hisF MCAODC_20450 0.48 +0.1 896,170 + hisF MCAODC_20450 0.48 -1.2 896,183 - hisF MCAODC_20450 0.49 +0.9 896,216 + hisF MCAODC_20450 0.54 -2.5 896,217 - hisF MCAODC_20450 0.54 -1.5 896,232 - hisF MCAODC_20450 0.56 -0.0 896,511 + -2.1 896,511 + -1.2 896,529 + -0.7 896,537 + -0.2 896,537 + +1.3 896,569 - +0.7 896,674 + hisIE MCAODC_20455 0.17 -1.5 896,676 + hisIE MCAODC_20455 0.17 -2.2 896,676 + hisIE MCAODC_20455 0.17 -0.9 896,693 + hisIE MCAODC_20455 0.20 +3.5 896,796 + hisIE MCAODC_20455 0.37 +3.5 896,968 + hisIE MCAODC_20455 0.65 +0.3 896,969 - hisIE MCAODC_20455 0.65 +2.5 896,983 + hisIE MCAODC_20455 0.68 +0.7 897,056 + hisIE MCAODC_20455 0.80 +0.0 897,057 - hisIE MCAODC_20455 0.80 +1.3 897,100 + hisIE MCAODC_20455 0.87 +2.9 897,165 + +0.2 897,165 + -1.4 897,291 - -0.7 897,291 - +0.2 897,291 - +0.8 897,397 - wzzB MCAODC_20460 0.12 -0.7 897,401 - wzzB MCAODC_20460 0.13 -0.1 897,416 - wzzB MCAODC_20460 0.14 +0.1 897,492 - wzzB MCAODC_20460 0.22 -0.1 897,495 - wzzB MCAODC_20460 0.22 +1.1 897,535 - wzzB MCAODC_20460 0.26 +1.8 897,535 - wzzB MCAODC_20460 0.26 +0.8 897,535 - wzzB MCAODC_20460 0.26 -1.9 897,577 - wzzB MCAODC_20460 0.31 +0.3 897,577 - wzzB MCAODC_20460 0.31 +0.9 897,577 - wzzB MCAODC_20460 0.31 +2.0 897,577 - wzzB MCAODC_20460 0.31 -0.1 897,639 - wzzB MCAODC_20460 0.37 +0.9 897,767 - wzzB MCAODC_20460 0.50 +0.7 897,804 - wzzB MCAODC_20460 0.54 -0.1 897,849 - wzzB MCAODC_20460 0.58 +0.0 897,849 - wzzB MCAODC_20460 0.58 -0.3 897,880 - wzzB MCAODC_20460 0.62 -0.8 898,014 - wzzB MCAODC_20460 0.75 -1.4 898,014 - wzzB MCAODC_20460 0.75 +0.3 898,014 - wzzB MCAODC_20460 0.75 -0.7 898,014 - wzzB MCAODC_20460 0.75 +1.3 898,014 - wzzB MCAODC_20460 0.75 -0.6 898,014 - wzzB MCAODC_20460 0.75 +0.9 898,018 - wzzB MCAODC_20460 0.76 +0.7 898,036 - wzzB MCAODC_20460 0.78 -0.2
Or see this region's nucleotide sequence