Experiment: Control_ECRC101
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebE and MCAODC_19035 are separated by 59 nucleotides MCAODC_19035 and MCAODC_19040 are separated by 36 nucleotides MCAODC_19040 and yebF overlap by 87 nucleotides yebF and yebG are separated by 66 nucleotides yebG and purT are separated by 133 nucleotides
MCAODC_19030: yebE - Inner membrane protein YebE, at 660,146 to 660,805
yebE
MCAODC_19035: MCAODC_19035 - hypothetical protein, at 660,865 to 660,975
_19035
MCAODC_19040: MCAODC_19040 - hypothetical protein, at 661,012 to 661,218
_19040
MCAODC_19045: yebF - Protein YebF, at 661,132 to 661,488
yebF
MCAODC_19050: yebG - DNA damage-inducible protein YebG, at 661,555 to 661,845
yebG
MCAODC_19055: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 661,979 to 663,157
purT
Position (kb) 661
662 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 660.244 kb on - strand, within yebE at 660.269 kb on - strand, within yebE at 660.294 kb on + strand, within yebE at 660.451 kb on + strand, within yebE at 660.451 kb on + strand, within yebE at 660.521 kb on - strand, within yebE at 660.577 kb on - strand, within yebE at 660.577 kb on - strand, within yebE at 660.639 kb on + strand, within yebE at 660.668 kb on - strand, within yebE at 660.668 kb on - strand, within yebE at 660.806 kb on - strand at 660.806 kb on - strand at 660.806 kb on - strand at 660.806 kb on - strand at 660.806 kb on - strand at 660.838 kb on + strand at 660.839 kb on - strand at 660.868 kb on + strand at 660.869 kb on - strand at 660.941 kb on + strand, within MCAODC_19035 at 660.942 kb on - strand, within MCAODC_19035 at 660.945 kb on + strand, within MCAODC_19035 at 660.946 kb on - strand, within MCAODC_19035 at 660.950 kb on + strand, within MCAODC_19035 at 660.951 kb on - strand, within MCAODC_19035 at 660.953 kb on - strand, within MCAODC_19035 at 660.953 kb on - strand, within MCAODC_19035 at 660.968 kb on + strand at 661.210 kb on + strand at 661.211 kb on - strand at 661.211 kb on - strand at 661.234 kb on - strand, within yebF at 661.528 kb on + strand at 661.764 kb on - strand, within yebG at 661.823 kb on - strand at 661.823 kb on - strand at 661.827 kb on - strand at 661.827 kb on - strand at 661.827 kb on - strand at 661.827 kb on - strand at 661.881 kb on + strand at 661.918 kb on - strand at 661.960 kb on + strand at 661.971 kb on + strand at 661.987 kb on - strand at 661.989 kb on + strand at 662.028 kb on + strand at 662.032 kb on - strand at 662.155 kb on + strand, within purT at 662.158 kb on + strand, within purT at 662.178 kb on + strand, within purT at 662.210 kb on + strand, within purT at 662.210 kb on + strand, within purT at 662.210 kb on + strand, within purT at 662.211 kb on - strand, within purT at 662.233 kb on + strand, within purT at 662.233 kb on + strand, within purT at 662.233 kb on + strand, within purT at 662.246 kb on - strand, within purT at 662.440 kb on - strand, within purT
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101 remove 660,244 - yebE MCAODC_19030 0.15 +1.3 660,269 - yebE MCAODC_19030 0.19 -1.0 660,294 + yebE MCAODC_19030 0.22 -0.1 660,451 + yebE MCAODC_19030 0.46 +0.4 660,451 + yebE MCAODC_19030 0.46 +0.7 660,521 - yebE MCAODC_19030 0.57 -0.8 660,577 - yebE MCAODC_19030 0.65 +0.8 660,577 - yebE MCAODC_19030 0.65 -0.8 660,639 + yebE MCAODC_19030 0.75 +1.9 660,668 - yebE MCAODC_19030 0.79 +2.0 660,668 - yebE MCAODC_19030 0.79 -1.4 660,806 - -0.1 660,806 - +0.2 660,806 - -0.5 660,806 - +0.2 660,806 - +1.4 660,838 + -0.1 660,839 - +0.3 660,868 + -0.3 660,869 - -0.7 660,941 + MCAODC_19035 0.68 +3.1 660,942 - MCAODC_19035 0.69 +0.0 660,945 + MCAODC_19035 0.72 +0.6 660,946 - MCAODC_19035 0.73 -0.2 660,950 + MCAODC_19035 0.77 -1.7 660,951 - MCAODC_19035 0.77 -1.7 660,953 - MCAODC_19035 0.79 +0.2 660,953 - MCAODC_19035 0.79 -0.8 660,968 + -1.2 661,210 + +1.6 661,211 - +1.9 661,211 - -2.3 661,234 - yebF MCAODC_19045 0.29 +0.6 661,528 + -0.4 661,764 - yebG MCAODC_19050 0.72 +0.7 661,823 - +0.1 661,823 - +0.6 661,827 - +1.3 661,827 - +0.4 661,827 - -0.4 661,827 - +1.1 661,881 + +0.5 661,918 - +1.7 661,960 + -0.3 661,971 + +0.5 661,987 - -1.1 661,989 + +0.9 662,028 + -0.3 662,032 - +1.2 662,155 + purT MCAODC_19055 0.15 +1.5 662,158 + purT MCAODC_19055 0.15 -0.6 662,178 + purT MCAODC_19055 0.17 +2.5 662,210 + purT MCAODC_19055 0.20 +0.9 662,210 + purT MCAODC_19055 0.20 +1.5 662,210 + purT MCAODC_19055 0.20 +0.5 662,211 - purT MCAODC_19055 0.20 -1.5 662,233 + purT MCAODC_19055 0.22 +0.2 662,233 + purT MCAODC_19055 0.22 +0.7 662,233 + purT MCAODC_19055 0.22 +1.5 662,246 - purT MCAODC_19055 0.23 -0.1 662,440 - purT MCAODC_19055 0.39 +0.9
Or see this region's nucleotide sequence