Experiment: Control_ECRC101
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-ISAs1 and pptA are separated by 98 nucleotides pptA and yddH are separated by 3 nucleotides yddH and yddH are separated by 3 nucleotides yddH and nhoA are separated by 172 nucleotides
MCAODC_16470: tnp-ISAs1 - ISAs1 family transposase, at 188,872 to 189,762
tnp-ISAs1
MCAODC_16475: pptA - tautomerase PptA, at 189,861 to 190,088
pptA
MCAODC_16480: yddH - Uncharacterized protein YddH, at 190,092 to 190,535
yddH
MCAODC_16485: yddH - Uncharacterized protein YddH, at 190,539 to 190,661
yddH
MCAODC_16490: nhoA - N-hydroxyarylamine O-acetyltransferase, at 190,834 to 191,568
nhoA
Position (kb) 189
190
191 Strain fitness (log2 ratio) -2
-1
0
1
2 at 189.777 kb on + strand at 189.777 kb on + strand at 189.878 kb on + strand at 189.879 kb on - strand at 190.022 kb on + strand, within pptA at 190.086 kb on + strand at 190.103 kb on + strand at 190.163 kb on + strand, within yddH at 190.247 kb on - strand, within yddH at 190.357 kb on + strand, within yddH at 190.371 kb on - strand, within yddH at 190.415 kb on + strand, within yddH at 190.415 kb on + strand, within yddH at 190.458 kb on - strand, within yddH at 190.491 kb on - strand at 190.554 kb on - strand, within yddH at 190.554 kb on - strand, within yddH at 190.614 kb on + strand, within yddH at 190.636 kb on - strand, within yddH at 190.641 kb on + strand, within yddH at 190.690 kb on - strand at 190.695 kb on + strand at 190.710 kb on + strand at 190.714 kb on + strand at 190.802 kb on + strand at 190.803 kb on - strand at 190.803 kb on - strand at 191.072 kb on + strand, within nhoA
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101 remove 189,777 + -2.4 189,777 + +0.9 189,878 + -0.1 189,879 - +1.4 190,022 + pptA MCAODC_16475 0.71 +1.3 190,086 + +1.1 190,103 + +1.5 190,163 + yddH MCAODC_16480 0.16 +1.4 190,247 - yddH MCAODC_16480 0.35 -0.1 190,357 + yddH MCAODC_16480 0.60 -0.1 190,371 - yddH MCAODC_16480 0.63 -0.3 190,415 + yddH MCAODC_16480 0.73 -2.3 190,415 + yddH MCAODC_16480 0.73 -0.7 190,458 - yddH MCAODC_16480 0.82 +0.0 190,491 - +0.7 190,554 - yddH MCAODC_16485 0.12 -0.1 190,554 - yddH MCAODC_16485 0.12 -2.4 190,614 + yddH MCAODC_16485 0.61 -0.9 190,636 - yddH MCAODC_16485 0.79 +0.3 190,641 + yddH MCAODC_16485 0.83 +0.5 190,690 - -1.2 190,695 + +1.9 190,710 + +0.2 190,714 + -1.7 190,802 + -0.3 190,803 - -1.3 190,803 - -0.7 191,072 + nhoA MCAODC_16490 0.32 -0.0
Or see this region's nucleotide sequence