Experiment: Control_ECRC101
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifJ and ydbJ are separated by 273 nucleotides ydbJ and hslJ overlap by 4 nucleotides hslJ and ldhA are separated by 110 nucleotides
MCAODC_16125: nifJ - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 112,325 to 115,849
nifJ
MCAODC_16130: ydbJ - Uncharacterized protein YdbJ, at 116,123 to 116,389
ydbJ
MCAODC_16135: hslJ - heat shock protein HslJ, at 116,386 to 116,808
hslJ
MCAODC_16140: ldhA - D-lactate dehydrogenase, at 116,919 to 117,908
ldhA
Position (kb)
116
117 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 115.143 kb on + strand, within nifJ at 115.144 kb on - strand, within nifJ at 115.144 kb on - strand, within nifJ at 115.155 kb on + strand, within nifJ at 115.188 kb on + strand, within nifJ at 115.610 kb on - strand at 115.844 kb on - strand at 115.844 kb on - strand at 115.844 kb on - strand at 115.849 kb on + strand at 115.849 kb on + strand at 115.860 kb on + strand at 115.860 kb on + strand at 115.861 kb on - strand at 115.861 kb on - strand at 115.861 kb on - strand at 115.861 kb on - strand at 115.861 kb on - strand at 115.904 kb on + strand at 115.905 kb on - strand at 115.908 kb on - strand at 115.972 kb on + strand at 115.973 kb on - strand at 116.076 kb on - strand at 116.078 kb on + strand at 116.078 kb on + strand at 116.079 kb on - strand at 116.079 kb on - strand at 116.158 kb on - strand, within ydbJ at 116.306 kb on - strand, within ydbJ at 116.385 kb on - strand at 116.385 kb on - strand at 116.396 kb on - strand at 116.409 kb on + strand at 116.409 kb on + strand at 116.409 kb on + strand at 116.410 kb on - strand at 116.416 kb on + strand at 116.416 kb on + strand at 116.417 kb on - strand at 116.417 kb on - strand at 116.417 kb on - strand at 116.538 kb on + strand, within hslJ at 116.683 kb on - strand, within hslJ at 116.720 kb on - strand, within hslJ at 116.823 kb on - strand at 116.843 kb on - strand at 116.878 kb on + strand at 116.879 kb on - strand at 116.965 kb on - strand at 117.141 kb on - strand, within ldhA
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101 remove 115,143 + nifJ MCAODC_16125 0.80 -0.3 115,144 - nifJ MCAODC_16125 0.80 -0.3 115,144 - nifJ MCAODC_16125 0.80 +0.4 115,155 + nifJ MCAODC_16125 0.80 +3.8 115,188 + nifJ MCAODC_16125 0.81 +2.1 115,610 - +1.5 115,844 - -0.8 115,844 - -1.0 115,844 - +0.3 115,849 + +1.1 115,849 + +0.4 115,860 + +1.3 115,860 + -1.1 115,861 - -1.2 115,861 - +2.8 115,861 - -0.1 115,861 - -1.1 115,861 - -1.0 115,904 + -0.2 115,905 - -2.3 115,908 - -0.3 115,972 + -1.1 115,973 - +1.0 116,076 - -0.7 116,078 + -0.8 116,078 + +0.2 116,079 - +2.0 116,079 - +1.1 116,158 - ydbJ MCAODC_16130 0.13 +1.4 116,306 - ydbJ MCAODC_16130 0.69 -2.0 116,385 - -0.7 116,385 - +2.5 116,396 - +0.7 116,409 + -2.4 116,409 + -1.7 116,409 + +1.2 116,410 - -0.2 116,416 + -1.9 116,416 + -0.8 116,417 - +1.1 116,417 - +0.9 116,417 - +0.8 116,538 + hslJ MCAODC_16135 0.36 -0.5 116,683 - hslJ MCAODC_16135 0.70 +0.4 116,720 - hslJ MCAODC_16135 0.79 -0.3 116,823 - -0.5 116,843 - -1.0 116,878 + -0.3 116,879 - -0.1 116,965 - +0.0 117,141 - ldhA MCAODC_16140 0.22 -0.1
Or see this region's nucleotide sequence