Strain Fitness in Escherichia coli ECRC101 around MCAODC_13180

Experiment: Control_ECRC101

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbet and MCAODC_13170 are separated by 5 nucleotidesMCAODC_13170 and MCAODC_13175 are separated by 54 nucleotidesMCAODC_13175 and cIII overlap by 32 nucleotidescIII and ea10 are separated by 72 nucleotidesea10 and MCAODC_13190 are separated by 150 nucleotides MCAODC_13165: bet - phage recombination protein Bet, at 2,600,968 to 2,601,753 bet MCAODC_13170: MCAODC_13170 - host-nuclease inhibitor protein Gam, at 2,601,759 to 2,602,055 _13170 MCAODC_13175: MCAODC_13175 - host cell division inhibitory peptide Kil, at 2,602,110 to 2,602,274 _13175 MCAODC_13180: cIII - Protease inhibitor III, at 2,602,243 to 2,602,407 cIII MCAODC_13185: ea10 - Protein ea10, at 2,602,480 to 2,602,848 ea10 MCAODC_13190: MCAODC_13190 - Prophage protein, at 2,602,999 to 2,603,469 _13190 Position (kb) 2602 2603Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2601.761 kb on - strandat 2601.761 kb on - strandat 2602.168 kb on - strand, within MCAODC_13175at 2602.259 kb on + strandat 2602.259 kb on + strandat 2602.260 kb on - strandat 2602.296 kb on + strand, within cIIIat 2602.424 kb on - strandat 2602.425 kb on + strandat 2602.425 kb on + strandat 2602.513 kb on - strandat 2602.528 kb on - strand, within ea10at 2602.540 kb on - strand, within ea10at 2602.685 kb on - strand, within ea10at 2602.694 kb on - strand, within ea10at 2602.775 kb on - strand, within ea10at 2602.799 kb on - strand, within ea10at 2602.818 kb on - strandat 2602.910 kb on + strandat 2603.068 kb on - strand, within MCAODC_13190at 2603.321 kb on + strand, within MCAODC_13190

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101
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2,601,761 - -1.1
2,601,761 - +0.1
2,602,168 - MCAODC_13175 0.35 +2.3
2,602,259 + -0.5
2,602,259 + +0.2
2,602,260 - -3.3
2,602,296 + cIII MCAODC_13180 0.32 +1.2
2,602,424 - -1.1
2,602,425 + +0.1
2,602,425 + +0.5
2,602,513 - +0.2
2,602,528 - ea10 MCAODC_13185 0.13 -1.8
2,602,540 - ea10 MCAODC_13185 0.16 -1.9
2,602,685 - ea10 MCAODC_13185 0.56 -0.7
2,602,694 - ea10 MCAODC_13185 0.58 -0.2
2,602,775 - ea10 MCAODC_13185 0.80 -2.4
2,602,799 - ea10 MCAODC_13185 0.86 +0.3
2,602,818 - -0.5
2,602,910 + -2.0
2,603,068 - MCAODC_13190 0.15 -0.1
2,603,321 + MCAODC_13190 0.68 +1.9

Or see this region's nucleotide sequence