Experiment: Control_ECRC101
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt espB and cesD2 are separated by 6 nucleotides cesD2 and escF are separated by 35 nucleotides escF and escG are separated by 5 nucleotides escG and espF are separated by 84 nucleotides
MCAODC_05940: espB - type III secretion system LEE translocon pore-forming subunit EspB, at 1,194,190 to 1,195,128
espB
MCAODC_05945: cesD2 - LcrR family type III secretion system chaperone CesD2, at 1,195,135 to 1,195,542
cesD2
MCAODC_05950: escF - type III secretion system LEE needle filament protein EscF, at 1,195,578 to 1,195,799
escF
MCAODC_05955: escG - type III secretion system LEE needle protein cochaperone EscG, at 1,195,805 to 1,196,083
escG
MCAODC_05960: espF - type III secretion system LEE effector EspF, at 1,196,168 to 1,196,914
espF
Position (kb)
1195
1196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1194.633 kb on + strand, within espB at 1194.633 kb on + strand, within espB at 1194.634 kb on - strand, within espB at 1195.005 kb on + strand, within espB at 1195.005 kb on + strand, within espB at 1195.006 kb on - strand, within espB at 1195.006 kb on - strand, within espB at 1195.006 kb on - strand, within espB at 1195.035 kb on + strand at 1195.035 kb on + strand at 1195.056 kb on + strand at 1195.056 kb on + strand at 1195.056 kb on + strand at 1195.056 kb on + strand at 1195.056 kb on + strand at 1195.056 kb on + strand at 1195.056 kb on + strand at 1195.056 kb on + strand at 1195.057 kb on - strand at 1195.058 kb on + strand at 1195.058 kb on + strand at 1195.058 kb on + strand at 1195.058 kb on + strand at 1195.059 kb on - strand at 1195.126 kb on + strand at 1195.126 kb on + strand at 1195.127 kb on - strand at 1195.168 kb on + strand at 1195.168 kb on + strand at 1195.169 kb on - strand at 1195.196 kb on + strand, within cesD2 at 1195.375 kb on + strand, within cesD2 at 1195.420 kb on - strand, within cesD2 at 1195.532 kb on + strand at 1195.619 kb on + strand, within escF at 1195.653 kb on - strand, within escF at 1195.828 kb on + strand at 1196.008 kb on + strand, within escG at 1196.086 kb on + strand at 1196.088 kb on + strand at 1196.109 kb on - strand at 1196.125 kb on + strand at 1196.126 kb on - strand at 1196.127 kb on + strand at 1196.146 kb on + strand at 1196.152 kb on + strand at 1196.152 kb on + strand at 1196.167 kb on + strand at 1196.173 kb on + strand at 1196.186 kb on - strand at 1196.198 kb on - strand at 1196.198 kb on - strand at 1196.217 kb on + strand at 1196.217 kb on + strand at 1196.217 kb on + strand at 1196.218 kb on - strand at 1196.218 kb on - strand at 1196.218 kb on - strand at 1196.218 kb on - strand at 1196.380 kb on + strand, within espF at 1196.380 kb on + strand, within espF at 1196.380 kb on + strand, within espF at 1196.381 kb on - strand, within espF at 1196.381 kb on - strand, within espF at 1196.521 kb on + strand, within espF at 1196.521 kb on + strand, within espF
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101 remove 1,194,633 + espB MCAODC_05940 0.47 +0.5 1,194,633 + espB MCAODC_05940 0.47 +0.5 1,194,634 - espB MCAODC_05940 0.47 +0.4 1,195,005 + espB MCAODC_05940 0.87 -3.0 1,195,005 + espB MCAODC_05940 0.87 +0.8 1,195,006 - espB MCAODC_05940 0.87 -0.4 1,195,006 - espB MCAODC_05940 0.87 -0.8 1,195,006 - espB MCAODC_05940 0.87 -0.6 1,195,035 + +0.7 1,195,035 + -0.6 1,195,056 + +0.6 1,195,056 + +0.3 1,195,056 + -0.8 1,195,056 + -0.8 1,195,056 + +0.4 1,195,056 + -0.9 1,195,056 + +0.3 1,195,056 + +0.4 1,195,057 - -0.3 1,195,058 + -0.2 1,195,058 + -0.8 1,195,058 + +4.3 1,195,058 + -1.8 1,195,059 - -0.1 1,195,126 + -1.0 1,195,126 + -0.3 1,195,127 - -1.2 1,195,168 + -0.5 1,195,168 + -1.9 1,195,169 - -0.3 1,195,196 + cesD2 MCAODC_05945 0.15 +0.3 1,195,375 + cesD2 MCAODC_05945 0.59 -1.8 1,195,420 - cesD2 MCAODC_05945 0.70 -0.7 1,195,532 + +0.2 1,195,619 + escF MCAODC_05950 0.18 +0.7 1,195,653 - escF MCAODC_05950 0.34 +0.7 1,195,828 + -0.5 1,196,008 + escG MCAODC_05955 0.73 -0.8 1,196,086 + +0.5 1,196,088 + -0.6 1,196,109 - -0.4 1,196,125 + +1.6 1,196,126 - -0.6 1,196,127 + -0.8 1,196,146 + +0.5 1,196,152 + -0.7 1,196,152 + -0.5 1,196,167 + -1.8 1,196,173 + +0.1 1,196,186 - -1.0 1,196,198 - +0.1 1,196,198 - +0.0 1,196,217 + +0.5 1,196,217 + -0.2 1,196,217 + -0.2 1,196,218 - +2.7 1,196,218 - -0.6 1,196,218 - -0.3 1,196,218 - -0.4 1,196,380 + espF MCAODC_05960 0.28 -0.9 1,196,380 + espF MCAODC_05960 0.28 +0.2 1,196,380 + espF MCAODC_05960 0.28 +1.8 1,196,381 - espF MCAODC_05960 0.29 -0.2 1,196,381 - espF MCAODC_05960 0.29 -0.7 1,196,521 + espF MCAODC_05960 0.47 -0.3 1,196,521 + espF MCAODC_05960 0.47 +0.2
Or see this region's nucleotide sequence