Experiment: Control_ECRC101
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ler and escE are separated by 14 nucleotides escE and cesAB are separated by 12 nucleotides cesAB and escK overlap by 4 nucleotides escK and escL overlap by 14 nucleotides
MCAODC_05775: ler - transcription regulator Ler, at 1,166,014 to 1,166,385
ler
MCAODC_05780: escE - chaperone for EscF, at 1,166,400 to 1,166,618
escE
MCAODC_05785: cesAB - chaperone for EspA and EspB, at 1,166,631 to 1,166,945
cesAB
MCAODC_05790: escK - type III secretion system sorting platform component, at 1,166,942 to 1,167,541
escK
MCAODC_05795: escL - negative regulator EscL, stator protein, at 1,167,528 to 1,168,181
escL
Position (kb)
1166
1167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1165.420 kb on + strand at 1165.443 kb on + strand at 1165.475 kb on - strand at 1165.479 kb on + strand at 1165.480 kb on - strand at 1165.480 kb on - strand at 1165.497 kb on - strand at 1165.499 kb on + strand at 1165.500 kb on - strand at 1165.504 kb on - strand at 1165.504 kb on - strand at 1165.504 kb on - strand at 1165.506 kb on - strand at 1165.506 kb on - strand at 1165.506 kb on - strand at 1165.508 kb on - strand at 1165.508 kb on - strand at 1165.508 kb on - strand at 1165.508 kb on - strand at 1165.508 kb on - strand at 1165.508 kb on - strand at 1165.508 kb on - strand at 1165.510 kb on - strand at 1165.567 kb on - strand at 1165.567 kb on - strand at 1165.567 kb on - strand at 1165.571 kb on - strand at 1165.575 kb on - strand at 1165.657 kb on + strand at 1165.658 kb on - strand at 1165.668 kb on - strand at 1165.689 kb on + strand at 1165.689 kb on + strand at 1165.695 kb on - strand at 1165.715 kb on - strand at 1165.715 kb on - strand at 1165.731 kb on - strand at 1165.733 kb on + strand at 1165.820 kb on - strand at 1165.946 kb on - strand at 1166.038 kb on - strand at 1166.062 kb on - strand, within ler at 1166.081 kb on + strand, within ler at 1166.159 kb on - strand, within ler at 1166.172 kb on - strand, within ler at 1166.187 kb on + strand, within ler at 1166.239 kb on + strand, within ler at 1166.239 kb on + strand, within ler at 1166.576 kb on + strand, within escE at 1166.577 kb on - strand, within escE at 1166.744 kb on - strand, within cesAB at 1166.805 kb on + strand, within cesAB at 1166.811 kb on + strand, within cesAB at 1166.927 kb on + strand at 1166.968 kb on + strand at 1167.073 kb on + strand, within escK at 1167.097 kb on + strand, within escK at 1167.242 kb on + strand, within escK at 1167.409 kb on - strand, within escK at 1167.481 kb on + strand, within escK at 1167.581 kb on + strand at 1167.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC101 remove 1,165,420 + -1.2 1,165,443 + +0.3 1,165,475 - +0.5 1,165,479 + +0.4 1,165,480 - +0.5 1,165,480 - +0.3 1,165,497 - +0.3 1,165,499 + +0.9 1,165,500 - +0.8 1,165,504 - +2.6 1,165,504 - +1.0 1,165,504 - +1.0 1,165,506 - -2.2 1,165,506 - +1.2 1,165,506 - -3.6 1,165,508 - -1.5 1,165,508 - -0.2 1,165,508 - -1.2 1,165,508 - +1.5 1,165,508 - +0.6 1,165,508 - -0.1 1,165,508 - +0.8 1,165,510 - -0.6 1,165,567 - +0.4 1,165,567 - -0.7 1,165,567 - +0.3 1,165,571 - -0.2 1,165,575 - -0.3 1,165,657 + -0.3 1,165,658 - -0.4 1,165,668 - -1.9 1,165,689 + -0.3 1,165,689 + -0.4 1,165,695 - -0.1 1,165,715 - -1.4 1,165,715 - +0.1 1,165,731 - +1.4 1,165,733 + +0.8 1,165,820 - -0.1 1,165,946 - -0.5 1,166,038 - -0.2 1,166,062 - ler MCAODC_05775 0.13 +0.7 1,166,081 + ler MCAODC_05775 0.18 -0.1 1,166,159 - ler MCAODC_05775 0.39 -2.4 1,166,172 - ler MCAODC_05775 0.42 -2.4 1,166,187 + ler MCAODC_05775 0.47 +0.2 1,166,239 + ler MCAODC_05775 0.60 +0.6 1,166,239 + ler MCAODC_05775 0.60 +1.0 1,166,576 + escE MCAODC_05780 0.80 +1.8 1,166,577 - escE MCAODC_05780 0.81 +0.2 1,166,744 - cesAB MCAODC_05785 0.36 +2.5 1,166,805 + cesAB MCAODC_05785 0.55 +0.4 1,166,811 + cesAB MCAODC_05785 0.57 -0.5 1,166,927 + +0.9 1,166,968 + -0.9 1,167,073 + escK MCAODC_05790 0.22 -2.9 1,167,097 + escK MCAODC_05790 0.26 -2.0 1,167,242 + escK MCAODC_05790 0.50 +0.1 1,167,409 - escK MCAODC_05790 0.78 -0.0 1,167,481 + escK MCAODC_05790 0.90 +0.3 1,167,581 + -0.1 1,167,582 - -0.3
Or see this region's nucleotide sequence