Strain Fitness in Escherichia coli ECRC101 around MCAODC_00035

Experiment: Control_ECRC101

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_00025 and MCAODC_00030 overlap by 14 nucleotidesMCAODC_00030 and MCAODC_00035 are separated by 321 nucleotidesMCAODC_00035 and MCAODC_00040 overlap by 4 nucleotidesMCAODC_00040 and rusA overlap by 4 nucleotidesrusA and ybcO overlap by 4 nucleotidesybcO and ninE overlap by 8 nucleotidesninE and ybcN overlap by 1 nucleotides MCAODC_00025: MCAODC_00025 - lysozyme, at 2,248 to 2,724 _00025 MCAODC_00030: MCAODC_00030 - Holin, at 2,711 to 3,016 _00030 MCAODC_00035: MCAODC_00035 - Antitermination protein Q, at 3,338 to 4,027 _00035 MCAODC_00040: MCAODC_00040 - Uncharacterized protein YlcG, at 4,024 to 4,164 _00040 MCAODC_00045: rusA - crossover junction endodeoxyribonuclease RusA, at 4,161 to 4,523 rusA MCAODC_00050: ybcO - Putative nuclease YbcO, at 4,520 to 4,810 ybcO MCAODC_00055: ninE - Protein ninE, at 4,803 to 4,973 ninE MCAODC_00060: ybcN - Uncharacterized protein YbcN, at 4,973 to 5,428 ybcN Position (kb) 3 4 5Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2.358 kb on + strand, within MCAODC_00025at 2.407 kb on - strand, within MCAODC_00025at 2.407 kb on - strand, within MCAODC_00025at 2.627 kb on + strand, within MCAODC_00025at 2.812 kb on - strand, within MCAODC_00030at 2.895 kb on - strand, within MCAODC_00030at 2.895 kb on - strand, within MCAODC_00030at 2.930 kb on + strand, within MCAODC_00030at 2.930 kb on + strand, within MCAODC_00030at 3.016 kb on + strandat 3.016 kb on + strandat 3.017 kb on - strandat 3.017 kb on - strandat 3.149 kb on + strandat 3.150 kb on - strandat 3.168 kb on - strandat 3.522 kb on + strand, within MCAODC_00035at 3.528 kb on + strand, within MCAODC_00035at 3.529 kb on - strand, within MCAODC_00035at 3.529 kb on - strand, within MCAODC_00035at 3.703 kb on - strand, within MCAODC_00035at 3.703 kb on - strand, within MCAODC_00035at 3.854 kb on - strand, within MCAODC_00035at 3.854 kb on - strand, within MCAODC_00035at 3.870 kb on - strand, within MCAODC_00035at 3.870 kb on - strand, within MCAODC_00035at 3.903 kb on + strand, within MCAODC_00035at 3.903 kb on + strand, within MCAODC_00035at 4.006 kb on - strandat 4.006 kb on - strandat 4.010 kb on + strandat 4.011 kb on - strandat 4.014 kb on + strandat 4.014 kb on + strandat 4.105 kb on + strand, within MCAODC_00040at 4.106 kb on - strand, within MCAODC_00040at 4.146 kb on + strand, within MCAODC_00040at 4.146 kb on + strand, within MCAODC_00040at 4.147 kb on - strand, within MCAODC_00040at 4.149 kb on - strand, within MCAODC_00040at 4.149 kb on - strand, within MCAODC_00040at 4.149 kb on - strand, within MCAODC_00040at 4.149 kb on - strand, within MCAODC_00040at 4.424 kb on - strand, within rusAat 4.424 kb on - strand, within rusAat 4.725 kb on - strand, within ybcOat 4.766 kb on + strand, within ybcOat 4.907 kb on + strand, within ninE

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC101
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2,358 + MCAODC_00025 0.23 +0.5
2,407 - MCAODC_00025 0.33 +1.5
2,407 - MCAODC_00025 0.33 -0.5
2,627 + MCAODC_00025 0.79 -1.1
2,812 - MCAODC_00030 0.33 -1.0
2,895 - MCAODC_00030 0.60 +0.8
2,895 - MCAODC_00030 0.60 +0.5
2,930 + MCAODC_00030 0.72 +0.0
2,930 + MCAODC_00030 0.72 +1.7
3,016 + +0.5
3,016 + -1.4
3,017 - -2.4
3,017 - -0.6
3,149 + -0.3
3,150 - -0.8
3,168 - -2.4
3,522 + MCAODC_00035 0.27 +0.2
3,528 + MCAODC_00035 0.28 +1.0
3,529 - MCAODC_00035 0.28 +0.5
3,529 - MCAODC_00035 0.28 +1.0
3,703 - MCAODC_00035 0.53 -1.0
3,703 - MCAODC_00035 0.53 +3.0
3,854 - MCAODC_00035 0.75 +0.1
3,854 - MCAODC_00035 0.75 +1.0
3,870 - MCAODC_00035 0.77 +2.7
3,870 - MCAODC_00035 0.77 -0.5
3,903 + MCAODC_00035 0.82 +0.6
3,903 + MCAODC_00035 0.82 +2.7
4,006 - +1.0
4,006 - +2.9
4,010 + +1.3
4,011 - +1.3
4,014 + +1.8
4,014 + -0.6
4,105 + MCAODC_00040 0.57 -0.6
4,106 - MCAODC_00040 0.58 +1.2
4,146 + MCAODC_00040 0.87 -0.0
4,146 + MCAODC_00040 0.87 +0.6
4,147 - MCAODC_00040 0.87 -0.5
4,149 - MCAODC_00040 0.89 +0.4
4,149 - MCAODC_00040 0.89 -0.9
4,149 - MCAODC_00040 0.89 -0.0
4,149 - MCAODC_00040 0.89 +0.5
4,424 - rusA MCAODC_00045 0.72 +3.1
4,424 - rusA MCAODC_00045 0.72 -0.6
4,725 - ybcO MCAODC_00050 0.70 +0.5
4,766 + ybcO MCAODC_00050 0.85 +0.1
4,907 + ninE MCAODC_00055 0.61 +1.4

Or see this region's nucleotide sequence