Experiment: Bas36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluE and nudJ are separated by 9 nucleotides nudJ and mnmA are separated by 53 nucleotides mnmA and hflD are separated by 35 nucleotides hflD and purB are separated by 3 nucleotides
JDDGAC_06610: rluE - 23S rRNA pseudouridine(2457) synthase RluE, at 1,152,488 to 1,153,141
rluE
JDDGAC_06615: nudJ - phosphatase NudJ, at 1,153,151 to 1,153,612
nudJ
JDDGAC_06620: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 1,153,666 to 1,154,772
mnmA
JDDGAC_06625: hflD - high frequency lysogenization protein HflD, at 1,154,808 to 1,155,449
hflD
JDDGAC_06630: purB - adenylosuccinate lyase, at 1,155,453 to 1,156,823
purB
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1152.704 kb on + strand, within rluE at 1152.704 kb on + strand, within rluE at 1152.812 kb on + strand, within rluE at 1152.812 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.880 kb on - strand, within rluE at 1153.002 kb on + strand, within rluE at 1153.002 kb on + strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.092 kb on - strand at 1153.139 kb on + strand at 1153.139 kb on + strand at 1153.351 kb on + strand, within nudJ at 1153.529 kb on - strand, within nudJ at 1153.529 kb on - strand, within nudJ at 1154.032 kb on + strand, within mnmA at 1154.123 kb on - strand, within mnmA at 1154.575 kb on - strand, within mnmA at 1154.710 kb on + strand at 1154.774 kb on + strand at 1154.777 kb on + strand at 1154.787 kb on + strand at 1154.933 kb on + strand, within hflD at 1154.934 kb on - strand, within hflD at 1154.942 kb on + strand, within hflD at 1155.049 kb on + strand, within hflD at 1155.215 kb on + strand, within hflD at 1155.219 kb on + strand, within hflD at 1155.219 kb on + strand, within hflD at 1155.219 kb on + strand, within hflD at 1155.393 kb on - strand at 1155.433 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas36 remove 1,152,704 + rluE JDDGAC_06610 0.33 -2.1 1,152,704 + rluE JDDGAC_06610 0.33 -0.8 1,152,812 + rluE JDDGAC_06610 0.50 -0.3 1,152,812 + rluE JDDGAC_06610 0.50 -0.7 1,152,815 + rluE JDDGAC_06610 0.50 -0.7 1,152,815 + rluE JDDGAC_06610 0.50 +1.3 1,152,815 + rluE JDDGAC_06610 0.50 +0.6 1,152,880 - rluE JDDGAC_06610 0.60 +2.3 1,153,002 + rluE JDDGAC_06610 0.79 -0.2 1,153,002 + rluE JDDGAC_06610 0.79 +0.5 1,153,003 - rluE JDDGAC_06610 0.79 -2.0 1,153,003 - rluE JDDGAC_06610 0.79 +2.1 1,153,003 - rluE JDDGAC_06610 0.79 -2.2 1,153,004 + rluE JDDGAC_06610 0.79 -0.1 1,153,004 + rluE JDDGAC_06610 0.79 -0.5 1,153,004 + rluE JDDGAC_06610 0.79 +2.7 1,153,004 + rluE JDDGAC_06610 0.79 -1.2 1,153,092 - -2.1 1,153,139 + -1.6 1,153,139 + +0.4 1,153,351 + nudJ JDDGAC_06615 0.43 -1.5 1,153,529 - nudJ JDDGAC_06615 0.82 -0.9 1,153,529 - nudJ JDDGAC_06615 0.82 +1.1 1,154,032 + mnmA JDDGAC_06620 0.33 -0.3 1,154,123 - mnmA JDDGAC_06620 0.41 -0.2 1,154,575 - mnmA JDDGAC_06620 0.82 -2.6 1,154,710 + -1.5 1,154,774 + -0.8 1,154,777 + +1.9 1,154,787 + -3.4 1,154,933 + hflD JDDGAC_06625 0.19 +0.3 1,154,934 - hflD JDDGAC_06625 0.20 -2.3 1,154,942 + hflD JDDGAC_06625 0.21 +2.7 1,155,049 + hflD JDDGAC_06625 0.38 +2.9 1,155,215 + hflD JDDGAC_06625 0.63 -0.5 1,155,219 + hflD JDDGAC_06625 0.64 +1.4 1,155,219 + hflD JDDGAC_06625 0.64 -1.2 1,155,219 + hflD JDDGAC_06625 0.64 +0.7 1,155,393 - +1.8 1,155,433 + -0.5
Or see this region's nucleotide sequence