Experiment: Bas36
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaA and rfaQ are separated by 412 nucleotides rfaQ and rfaG overlap by 4 nucleotides
JDDGAC_19270: waaA - lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase, at 3,664,333 to 3,665,610
waaA
JDDGAC_19275: rfaQ - lipopolysaccharide core heptosyltransferase RfaQ, at 3,666,023 to 3,667,081
rfaQ
JDDGAC_19280: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 3,667,078 to 3,668,202
rfaG
Position (kb)
3666
3667
3668 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3665.627 kb on - strand at 3665.647 kb on - strand at 3665.667 kb on - strand at 3665.673 kb on - strand at 3665.692 kb on + strand at 3665.692 kb on + strand at 3665.692 kb on + strand at 3665.692 kb on + strand at 3665.730 kb on - strand at 3665.730 kb on - strand at 3665.748 kb on + strand at 3665.905 kb on + strand at 3665.906 kb on - strand at 3665.906 kb on - strand at 3666.029 kb on - strand at 3666.076 kb on + strand at 3666.077 kb on - strand at 3666.088 kb on + strand at 3666.100 kb on + strand at 3666.100 kb on + strand at 3666.268 kb on - strand, within rfaQ at 3666.268 kb on - strand, within rfaQ at 3666.270 kb on + strand, within rfaQ at 3666.270 kb on + strand, within rfaQ at 3666.271 kb on - strand, within rfaQ at 3666.271 kb on - strand, within rfaQ at 3666.271 kb on - strand, within rfaQ at 3666.280 kb on + strand, within rfaQ at 3666.280 kb on + strand, within rfaQ at 3666.284 kb on + strand, within rfaQ at 3666.325 kb on + strand, within rfaQ at 3666.325 kb on + strand, within rfaQ at 3666.326 kb on - strand, within rfaQ at 3666.326 kb on - strand, within rfaQ at 3666.328 kb on - strand, within rfaQ at 3666.451 kb on + strand, within rfaQ at 3666.535 kb on + strand, within rfaQ at 3666.575 kb on + strand, within rfaQ at 3666.575 kb on + strand, within rfaQ at 3666.592 kb on + strand, within rfaQ at 3666.593 kb on - strand, within rfaQ at 3666.654 kb on + strand, within rfaQ at 3666.655 kb on - strand, within rfaQ at 3666.655 kb on - strand, within rfaQ at 3666.697 kb on + strand, within rfaQ at 3666.866 kb on - strand, within rfaQ at 3666.998 kb on + strand at 3666.999 kb on - strand at 3667.042 kb on + strand at 3667.042 kb on + strand at 3667.069 kb on + strand at 3667.078 kb on - strand at 3667.098 kb on - strand at 3667.102 kb on - strand at 3667.220 kb on + strand, within rfaG at 3667.280 kb on - strand, within rfaG at 3667.512 kb on + strand, within rfaG at 3667.566 kb on + strand, within rfaG at 3667.571 kb on + strand, within rfaG at 3667.572 kb on - strand, within rfaG at 3667.666 kb on + strand, within rfaG at 3667.671 kb on - strand, within rfaG at 3667.701 kb on + strand, within rfaG at 3667.769 kb on + strand, within rfaG at 3667.769 kb on + strand, within rfaG at 3667.769 kb on + strand, within rfaG at 3667.770 kb on - strand, within rfaG at 3667.771 kb on + strand, within rfaG at 3667.950 kb on + strand, within rfaG at 3667.967 kb on + strand, within rfaG at 3667.999 kb on + strand, within rfaG at 3668.000 kb on - strand, within rfaG at 3668.000 kb on - strand, within rfaG at 3668.002 kb on - strand, within rfaG at 3668.002 kb on - strand, within rfaG
Per-strain Table
Position Strand Gene LocusTag Fraction Bas36 remove 3,665,627 - +0.9 3,665,647 - +3.2 3,665,667 - +2.0 3,665,673 - +2.3 3,665,692 + +0.6 3,665,692 + +0.9 3,665,692 + +1.3 3,665,692 + +2.5 3,665,730 - +1.8 3,665,730 - -0.7 3,665,748 + +3.0 3,665,905 + +0.7 3,665,906 - -2.7 3,665,906 - -1.1 3,666,029 - +0.1 3,666,076 + +4.5 3,666,077 - +0.7 3,666,088 + +4.2 3,666,100 + +4.2 3,666,100 + +5.2 3,666,268 - rfaQ JDDGAC_19275 0.23 +0.8 3,666,268 - rfaQ JDDGAC_19275 0.23 +0.7 3,666,270 + rfaQ JDDGAC_19275 0.23 +4.5 3,666,270 + rfaQ JDDGAC_19275 0.23 +4.1 3,666,271 - rfaQ JDDGAC_19275 0.23 +0.2 3,666,271 - rfaQ JDDGAC_19275 0.23 -0.7 3,666,271 - rfaQ JDDGAC_19275 0.23 -0.7 3,666,280 + rfaQ JDDGAC_19275 0.24 +2.2 3,666,280 + rfaQ JDDGAC_19275 0.24 +2.9 3,666,284 + rfaQ JDDGAC_19275 0.25 +3.0 3,666,325 + rfaQ JDDGAC_19275 0.29 +4.0 3,666,325 + rfaQ JDDGAC_19275 0.29 +4.0 3,666,326 - rfaQ JDDGAC_19275 0.29 -0.5 3,666,326 - rfaQ JDDGAC_19275 0.29 -0.2 3,666,328 - rfaQ JDDGAC_19275 0.29 -0.1 3,666,451 + rfaQ JDDGAC_19275 0.40 +4.5 3,666,535 + rfaQ JDDGAC_19275 0.48 +4.7 3,666,575 + rfaQ JDDGAC_19275 0.52 +5.6 3,666,575 + rfaQ JDDGAC_19275 0.52 +4.1 3,666,592 + rfaQ JDDGAC_19275 0.54 +4.0 3,666,593 - rfaQ JDDGAC_19275 0.54 +2.2 3,666,654 + rfaQ JDDGAC_19275 0.60 +4.6 3,666,655 - rfaQ JDDGAC_19275 0.60 +0.3 3,666,655 - rfaQ JDDGAC_19275 0.60 -0.3 3,666,697 + rfaQ JDDGAC_19275 0.64 +4.4 3,666,866 - rfaQ JDDGAC_19275 0.80 -0.8 3,666,998 + +2.4 3,666,999 - -1.1 3,667,042 + -0.4 3,667,042 + +0.3 3,667,069 + +0.3 3,667,078 - +1.6 3,667,098 - -0.4 3,667,102 - -1.3 3,667,220 + rfaG JDDGAC_19280 0.13 +1.9 3,667,280 - rfaG JDDGAC_19280 0.18 +0.7 3,667,512 + rfaG JDDGAC_19280 0.39 -0.1 3,667,566 + rfaG JDDGAC_19280 0.43 -0.9 3,667,571 + rfaG JDDGAC_19280 0.44 -0.1 3,667,572 - rfaG JDDGAC_19280 0.44 +1.3 3,667,666 + rfaG JDDGAC_19280 0.52 +1.7 3,667,671 - rfaG JDDGAC_19280 0.53 +0.6 3,667,701 + rfaG JDDGAC_19280 0.55 +1.8 3,667,769 + rfaG JDDGAC_19280 0.61 -0.4 3,667,769 + rfaG JDDGAC_19280 0.61 +0.6 3,667,769 + rfaG JDDGAC_19280 0.61 +1.2 3,667,770 - rfaG JDDGAC_19280 0.62 +2.9 3,667,771 + rfaG JDDGAC_19280 0.62 -0.1 3,667,950 + rfaG JDDGAC_19280 0.78 -0.1 3,667,967 + rfaG JDDGAC_19280 0.79 +1.4 3,667,999 + rfaG JDDGAC_19280 0.82 -0.1 3,668,000 - rfaG JDDGAC_19280 0.82 +1.1 3,668,000 - rfaG JDDGAC_19280 0.82 +0.9 3,668,002 - rfaG JDDGAC_19280 0.82 +1.9 3,668,002 - rfaG JDDGAC_19280 0.82 +1.1
Or see this region's nucleotide sequence