Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt psiB and psiA overlap by 4 nucleotides psiA and flmC are separated by 173 nucleotides flmC and JDDGAC_30085 are separated by 185 nucleotides
JDDGAC_30065: psiB - conjugation system SOS inhibitor PsiB, at 34,232 to 34,666
psiB
JDDGAC_30070: psiA - Protein PsiA, at 34,663 to 35,424
psiA
JDDGAC_30080: flmC - Protein FlmC, at 35,598 to 35,810
flmC
JDDGAC_30085: JDDGAC_30085 - NikB, at 35,996 to 37,969
_30085
Position (kb) 35
36 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 34.614 kb on - strand, within psiB at 34.663 kb on - strand at 35.044 kb on + strand, within psiA at 35.089 kb on + strand, within psiA at 35.268 kb on + strand, within psiA at 35.269 kb on - strand, within psiA at 35.467 kb on + strand at 35.467 kb on + strand at 35.468 kb on - strand at 35.468 kb on - strand at 35.468 kb on - strand at 35.468 kb on - strand at 35.469 kb on + strand at 35.470 kb on - strand at 35.476 kb on + strand at 35.717 kb on + strand, within flmC at 35.798 kb on - strand at 35.812 kb on + strand at 35.812 kb on + strand at 35.812 kb on + strand at 35.934 kb on + strand at 35.934 kb on + strand at 35.934 kb on + strand at 35.935 kb on - strand at 35.935 kb on - strand at 35.984 kb on + strand at 35.984 kb on + strand at 35.985 kb on - strand at 35.985 kb on - strand at 35.986 kb on + strand at 36.074 kb on + strand at 36.074 kb on + strand at 36.074 kb on + strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.075 kb on - strand at 36.077 kb on - strand at 36.077 kb on - strand at 36.220 kb on + strand, within JDDGAC_30085 at 36.221 kb on - strand, within JDDGAC_30085 at 36.320 kb on + strand, within JDDGAC_30085 at 36.420 kb on + strand, within JDDGAC_30085 at 36.420 kb on + strand, within JDDGAC_30085 at 36.421 kb on - strand, within JDDGAC_30085 at 36.525 kb on - strand, within JDDGAC_30085 at 36.525 kb on - strand, within JDDGAC_30085 at 36.525 kb on - strand, within JDDGAC_30085 at 36.525 kb on - strand, within JDDGAC_30085 at 36.525 kb on - strand, within JDDGAC_30085 at 36.526 kb on + strand, within JDDGAC_30085 at 36.526 kb on + strand, within JDDGAC_30085 at 36.526 kb on + strand, within JDDGAC_30085 at 36.527 kb on - strand, within JDDGAC_30085 at 36.559 kb on - strand, within JDDGAC_30085 at 36.583 kb on + strand, within JDDGAC_30085 at 36.610 kb on + strand, within JDDGAC_30085 at 36.610 kb on + strand, within JDDGAC_30085 at 36.610 kb on + strand, within JDDGAC_30085 at 36.611 kb on - strand, within JDDGAC_30085 at 36.611 kb on - strand, within JDDGAC_30085 at 36.619 kb on + strand, within JDDGAC_30085 at 36.638 kb on + strand, within JDDGAC_30085 at 36.652 kb on + strand, within JDDGAC_30085 at 36.685 kb on - strand, within JDDGAC_30085 at 36.725 kb on + strand, within JDDGAC_30085 at 36.760 kb on + strand, within JDDGAC_30085
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 34,614 - psiB JDDGAC_30065 0.88 +1.3 34,663 - -0.1 35,044 + psiA JDDGAC_30070 0.50 -0.2 35,089 + psiA JDDGAC_30070 0.56 -3.4 35,268 + psiA JDDGAC_30070 0.79 +0.1 35,269 - psiA JDDGAC_30070 0.80 -1.9 35,467 + +0.4 35,467 + +0.4 35,468 - -0.4 35,468 - +0.8 35,468 - +0.1 35,468 - -0.5 35,469 + +1.5 35,470 - -1.7 35,476 + +1.4 35,717 + flmC JDDGAC_30080 0.56 -2.8 35,798 - -1.7 35,812 + +0.5 35,812 + -0.3 35,812 + -1.0 35,934 + -0.6 35,934 + -0.9 35,934 + -0.3 35,935 - -0.6 35,935 - +1.5 35,984 + +2.9 35,984 + +0.2 35,985 - -1.3 35,985 - +1.6 35,986 + -0.3 36,074 + -0.5 36,074 + +1.0 36,074 + +0.4 36,075 - -0.2 36,075 - +0.3 36,075 - +0.7 36,075 - +0.0 36,077 - +1.8 36,077 - +0.3 36,220 + JDDGAC_30085 0.11 +0.4 36,221 - JDDGAC_30085 0.11 -0.8 36,320 + JDDGAC_30085 0.16 -0.5 36,420 + JDDGAC_30085 0.21 +0.5 36,420 + JDDGAC_30085 0.21 +0.8 36,421 - JDDGAC_30085 0.22 -0.6 36,525 - JDDGAC_30085 0.27 +0.7 36,525 - JDDGAC_30085 0.27 -0.5 36,525 - JDDGAC_30085 0.27 -2.3 36,525 - JDDGAC_30085 0.27 -1.0 36,525 - JDDGAC_30085 0.27 -3.2 36,526 + JDDGAC_30085 0.27 +0.0 36,526 + JDDGAC_30085 0.27 -0.0 36,526 + JDDGAC_30085 0.27 -0.3 36,527 - JDDGAC_30085 0.27 +0.3 36,559 - JDDGAC_30085 0.29 +0.0 36,583 + JDDGAC_30085 0.30 +0.6 36,610 + JDDGAC_30085 0.31 -0.7 36,610 + JDDGAC_30085 0.31 +0.7 36,610 + JDDGAC_30085 0.31 +1.3 36,611 - JDDGAC_30085 0.31 +0.5 36,611 - JDDGAC_30085 0.31 -0.5 36,619 + JDDGAC_30085 0.32 +0.8 36,638 + JDDGAC_30085 0.33 +0.4 36,652 + JDDGAC_30085 0.33 -0.4 36,685 - JDDGAC_30085 0.35 -0.6 36,725 + JDDGAC_30085 0.37 +0.0 36,760 + JDDGAC_30085 0.39 -0.5
Or see this region's nucleotide sequence