Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctI and escJ overlap by 4 nucleotides escJ and JDDGAC_23480 are separated by 15 nucleotides JDDGAC_23480 and ygeN overlap by 59 nucleotides ygeN and ygeN are separated by 16 nucleotides
JDDGAC_23470: sctI - type III secretion system inner rod subunit SctI, at 4,497,724 to 4,498,056
sctI
JDDGAC_23475: escJ - EscJ/YscJ/HrcJ family type III secretion inner membrane ring protein, at 4,498,053 to 4,498,787
escJ
JDDGAC_23480: JDDGAC_23480 - type III secretion apparatus protein OrgA/MxiK, at 4,498,803 to 4,499,384
_23480
JDDGAC_23485: ygeN - Putative uncharacterized protein YgeN, at 4,499,326 to 4,499,586
ygeN
JDDGAC_23490: ygeN - Putative uncharacterized protein YgeN, at 4,499,603 to 4,500,034
ygeN
Position (kb) 4498
4499
4500 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4497.868 kb on + strand, within sctI at 4497.975 kb on + strand, within sctI at 4498.059 kb on + strand at 4498.176 kb on - strand, within escJ at 4498.695 kb on - strand, within escJ at 4499.002 kb on + strand, within JDDGAC_23480 at 4499.163 kb on + strand, within JDDGAC_23480 at 4499.207 kb on + strand, within JDDGAC_23480 at 4499.722 kb on + strand, within ygeN at 4499.725 kb on - strand, within ygeN at 4499.757 kb on + strand, within ygeN at 4500.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 4,497,868 + sctI JDDGAC_23470 0.43 -1.1 4,497,975 + sctI JDDGAC_23470 0.75 +0.8 4,498,059 + +3.5 4,498,176 - escJ JDDGAC_23475 0.17 +1.8 4,498,695 - escJ JDDGAC_23475 0.87 -0.0 4,499,002 + JDDGAC_23480 0.34 -0.7 4,499,163 + JDDGAC_23480 0.62 +0.0 4,499,207 + JDDGAC_23480 0.69 -2.5 4,499,722 + ygeN JDDGAC_23490 0.28 +1.7 4,499,725 - ygeN JDDGAC_23490 0.28 -0.5 4,499,757 + ygeN JDDGAC_23490 0.36 +2.0 4,500,342 + -1.2
Or see this region's nucleotide sequence