Experiment: WM_TP6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfL and mlc are separated by 134 nucleotides mlc and bioD are separated by 124 nucleotides bioD and clcB overlap by 48 nucleotides
JDDGAC_02325: ynfL - Uncharacterized HTH-type transcriptional regulator YnfL, at 421,160 to 422,053
ynfL
JDDGAC_02330: mlc - DNA-binding transcriptional repressor Mlc, at 422,188 to 423,408
mlc
JDDGAC_02335: bioD - dethiobiotin synthase, at 423,533 to 424,228
bioD
JDDGAC_02340: clcB - voltage-gated ClC-type chloride channel ClcB, at 424,181 to 425,437
clcB
Position (kb) 422
423
424 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 421.188 kb on - strand at 421.329 kb on + strand, within ynfL at 421.330 kb on - strand, within ynfL at 421.330 kb on - strand, within ynfL at 421.609 kb on + strand, within ynfL at 421.699 kb on + strand, within ynfL at 421.906 kb on + strand, within ynfL at 422.018 kb on - strand at 422.027 kb on - strand at 422.125 kb on + strand at 422.225 kb on + strand at 422.922 kb on - strand, within mlc at 422.949 kb on + strand, within mlc at 422.949 kb on + strand, within mlc at 423.152 kb on + strand, within mlc at 423.182 kb on + strand, within mlc at 423.235 kb on - strand, within mlc at 423.258 kb on + strand, within mlc at 423.287 kb on - strand at 423.324 kb on + strand at 423.471 kb on - strand at 423.489 kb on - strand at 423.504 kb on - strand at 423.604 kb on - strand, within bioD at 423.748 kb on + strand, within bioD at 423.748 kb on + strand, within bioD at 423.749 kb on - strand, within bioD at 423.886 kb on + strand, within bioD at 424.137 kb on - strand, within bioD at 424.172 kb on + strand at 424.182 kb on + strand at 424.183 kb on - strand at 424.183 kb on - strand at 424.186 kb on + strand at 424.213 kb on + strand at 424.213 kb on + strand at 424.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP6 remove 421,188 - +0.4 421,329 + ynfL JDDGAC_02325 0.19 +1.2 421,330 - ynfL JDDGAC_02325 0.19 +0.0 421,330 - ynfL JDDGAC_02325 0.19 -1.2 421,609 + ynfL JDDGAC_02325 0.50 -0.5 421,699 + ynfL JDDGAC_02325 0.60 +0.9 421,906 + ynfL JDDGAC_02325 0.83 +2.2 422,018 - +0.5 422,027 - -1.8 422,125 + +0.3 422,225 + -0.5 422,922 - mlc JDDGAC_02330 0.60 -0.8 422,949 + mlc JDDGAC_02330 0.62 -1.3 422,949 + mlc JDDGAC_02330 0.62 +0.5 423,152 + mlc JDDGAC_02330 0.79 +1.0 423,182 + mlc JDDGAC_02330 0.81 -1.7 423,235 - mlc JDDGAC_02330 0.86 -3.6 423,258 + mlc JDDGAC_02330 0.88 -2.2 423,287 - +1.8 423,324 + -3.1 423,471 - +3.2 423,489 - +0.8 423,504 - -0.9 423,604 - bioD JDDGAC_02335 0.10 -1.4 423,748 + bioD JDDGAC_02335 0.31 -0.8 423,748 + bioD JDDGAC_02335 0.31 -0.2 423,749 - bioD JDDGAC_02335 0.31 +1.9 423,886 + bioD JDDGAC_02335 0.51 +0.5 424,137 - bioD JDDGAC_02335 0.87 +0.4 424,172 + -0.4 424,182 + +0.8 424,183 - +0.4 424,183 - -0.4 424,186 + +0.5 424,213 + -0.4 424,213 + +0.4 424,281 - -1.9
Or see this region's nucleotide sequence