Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28625

Experiment: Control_ECRC98_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisC and hisD overlap by 4 nucleotideshisD and hisG are separated by 5 nucleotides JDDGAC_28620: hisC - histidinol-phosphate transaminase, at 5,493,703 to 5,494,773 hisC JDDGAC_28625: hisD - Histidinol dehydrogenase, at 5,494,770 to 5,496,074 hisD JDDGAC_28630: hisG - ATP phosphoribosyltransferase, at 5,496,080 to 5,496,979 hisG Position (kb) 5494 5495 5496 5497Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 5493.817 kb on - strand, within hisCat 5493.878 kb on + strand, within hisCat 5493.879 kb on - strand, within hisCat 5493.879 kb on - strand, within hisCat 5494.214 kb on + strand, within hisCat 5494.214 kb on + strand, within hisCat 5494.214 kb on + strand, within hisCat 5494.322 kb on + strand, within hisCat 5494.323 kb on - strand, within hisCat 5494.323 kb on - strand, within hisCat 5494.363 kb on + strand, within hisCat 5494.436 kb on + strand, within hisCat 5494.437 kb on - strand, within hisCat 5494.512 kb on + strand, within hisCat 5494.555 kb on + strand, within hisCat 5494.556 kb on - strand, within hisCat 5494.758 kb on + strandat 5494.785 kb on - strandat 5494.805 kb on + strandat 5494.881 kb on + strandat 5494.882 kb on - strandat 5494.954 kb on + strand, within hisDat 5494.954 kb on + strand, within hisDat 5494.955 kb on - strand, within hisDat 5494.993 kb on + strand, within hisDat 5495.266 kb on - strand, within hisDat 5495.646 kb on + strand, within hisDat 5495.646 kb on + strand, within hisDat 5495.650 kb on - strand, within hisDat 5495.686 kb on + strand, within hisDat 5495.686 kb on + strand, within hisDat 5495.687 kb on - strand, within hisDat 5495.754 kb on + strand, within hisDat 5495.824 kb on - strand, within hisDat 5495.913 kb on - strand, within hisDat 5496.045 kb on - strandat 5496.298 kb on - strand, within hisGat 5496.393 kb on - strand, within hisGat 5496.415 kb on - strand, within hisGat 5496.415 kb on - strand, within hisGat 5496.418 kb on + strand, within hisGat 5496.418 kb on + strand, within hisGat 5496.418 kb on + strand, within hisGat 5496.568 kb on - strand, within hisGat 5496.702 kb on + strand, within hisGat 5496.703 kb on - strand, within hisGat 5496.733 kb on + strand, within hisGat 5496.779 kb on + strand, within hisGat 5496.833 kb on - strand, within hisGat 5496.950 kb on - strandat 5496.953 kb on + strandat 5496.986 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98_WM
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5,493,817 - hisC JDDGAC_28620 0.11 -0.4
5,493,878 + hisC JDDGAC_28620 0.16 -2.7
5,493,879 - hisC JDDGAC_28620 0.16 -3.2
5,493,879 - hisC JDDGAC_28620 0.16 -2.9
5,494,214 + hisC JDDGAC_28620 0.48 +0.0
5,494,214 + hisC JDDGAC_28620 0.48 -0.7
5,494,214 + hisC JDDGAC_28620 0.48 +0.7
5,494,322 + hisC JDDGAC_28620 0.58 -2.6
5,494,323 - hisC JDDGAC_28620 0.58 -1.4
5,494,323 - hisC JDDGAC_28620 0.58 -3.3
5,494,363 + hisC JDDGAC_28620 0.62 -1.0
5,494,436 + hisC JDDGAC_28620 0.68 -1.6
5,494,437 - hisC JDDGAC_28620 0.69 -1.7
5,494,512 + hisC JDDGAC_28620 0.76 +0.2
5,494,555 + hisC JDDGAC_28620 0.80 -0.1
5,494,556 - hisC JDDGAC_28620 0.80 -3.8
5,494,758 + -2.4
5,494,785 - -0.4
5,494,805 + -0.6
5,494,881 + -1.9
5,494,882 - -2.1
5,494,954 + hisD JDDGAC_28625 0.14 -1.6
5,494,954 + hisD JDDGAC_28625 0.14 -0.1
5,494,955 - hisD JDDGAC_28625 0.14 -2.6
5,494,993 + hisD JDDGAC_28625 0.17 -2.7
5,495,266 - hisD JDDGAC_28625 0.38 -2.1
5,495,646 + hisD JDDGAC_28625 0.67 -1.1
5,495,646 + hisD JDDGAC_28625 0.67 -1.2
5,495,650 - hisD JDDGAC_28625 0.67 -0.8
5,495,686 + hisD JDDGAC_28625 0.70 -0.7
5,495,686 + hisD JDDGAC_28625 0.70 +3.8
5,495,687 - hisD JDDGAC_28625 0.70 -2.7
5,495,754 + hisD JDDGAC_28625 0.75 -0.7
5,495,824 - hisD JDDGAC_28625 0.81 -1.2
5,495,913 - hisD JDDGAC_28625 0.88 -1.3
5,496,045 - -1.5
5,496,298 - hisG JDDGAC_28630 0.24 -1.2
5,496,393 - hisG JDDGAC_28630 0.35 -1.0
5,496,415 - hisG JDDGAC_28630 0.37 +0.2
5,496,415 - hisG JDDGAC_28630 0.37 -2.7
5,496,418 + hisG JDDGAC_28630 0.38 -0.8
5,496,418 + hisG JDDGAC_28630 0.38 -1.2
5,496,418 + hisG JDDGAC_28630 0.38 -2.3
5,496,568 - hisG JDDGAC_28630 0.54 +1.0
5,496,702 + hisG JDDGAC_28630 0.69 +1.3
5,496,703 - hisG JDDGAC_28630 0.69 +0.2
5,496,733 + hisG JDDGAC_28630 0.73 +1.2
5,496,779 + hisG JDDGAC_28630 0.78 -1.0
5,496,833 - hisG JDDGAC_28630 0.84 -0.7
5,496,950 - -2.5
5,496,953 + -1.1
5,496,986 - -1.1

Or see this region's nucleotide sequence