Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28615

Experiment: Control_ECRC98_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisA and hisH overlap by 1 nucleotideshisH and hisB overlap by 1 nucleotideshisB and hisC overlap by 1 nucleotides JDDGAC_28605: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 5,491,309 to 5,492,046 hisA JDDGAC_28610: hisH - imidazole glycerol phosphate synthase subunit HisH, at 5,492,046 to 5,492,636 hisH JDDGAC_28615: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 5,492,636 to 5,493,703 hisB JDDGAC_28620: hisC - histidinol-phosphate transaminase, at 5,493,703 to 5,494,773 hisC Position (kb) 5492 5493 5494Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5491.851 kb on + strand, within hisAat 5492.024 kb on - strandat 5492.127 kb on - strand, within hisHat 5492.161 kb on + strand, within hisHat 5492.165 kb on + strand, within hisHat 5492.166 kb on - strand, within hisHat 5492.564 kb on - strand, within hisHat 5492.616 kb on - strandat 5492.637 kb on + strandat 5492.637 kb on + strandat 5492.746 kb on + strand, within hisBat 5492.784 kb on + strand, within hisBat 5492.844 kb on + strand, within hisBat 5492.860 kb on + strand, within hisBat 5493.031 kb on - strand, within hisBat 5493.031 kb on - strand, within hisBat 5493.121 kb on + strand, within hisBat 5493.192 kb on - strand, within hisBat 5493.467 kb on + strand, within hisBat 5493.658 kb on + strandat 5493.691 kb on - strandat 5493.691 kb on - strandat 5493.691 kb on - strandat 5493.817 kb on - strand, within hisCat 5493.878 kb on + strand, within hisCat 5493.879 kb on - strand, within hisCat 5493.879 kb on - strand, within hisCat 5494.214 kb on + strand, within hisCat 5494.214 kb on + strand, within hisCat 5494.214 kb on + strand, within hisCat 5494.322 kb on + strand, within hisCat 5494.323 kb on - strand, within hisCat 5494.323 kb on - strand, within hisCat 5494.363 kb on + strand, within hisCat 5494.436 kb on + strand, within hisCat 5494.437 kb on - strand, within hisCat 5494.512 kb on + strand, within hisCat 5494.555 kb on + strand, within hisCat 5494.556 kb on - strand, within hisC

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98_WM
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5,491,851 + hisA JDDGAC_28605 0.73 -3.1
5,492,024 - -0.3
5,492,127 - hisH JDDGAC_28610 0.14 -4.1
5,492,161 + hisH JDDGAC_28610 0.19 -1.7
5,492,165 + hisH JDDGAC_28610 0.20 -1.6
5,492,166 - hisH JDDGAC_28610 0.20 -2.4
5,492,564 - hisH JDDGAC_28610 0.88 -3.1
5,492,616 - -3.4
5,492,637 + -4.1
5,492,637 + -1.7
5,492,746 + hisB JDDGAC_28615 0.10 -2.1
5,492,784 + hisB JDDGAC_28615 0.14 -1.0
5,492,844 + hisB JDDGAC_28615 0.19 -1.7
5,492,860 + hisB JDDGAC_28615 0.21 -2.9
5,493,031 - hisB JDDGAC_28615 0.37 -1.9
5,493,031 - hisB JDDGAC_28615 0.37 +2.0
5,493,121 + hisB JDDGAC_28615 0.45 -2.2
5,493,192 - hisB JDDGAC_28615 0.52 -0.2
5,493,467 + hisB JDDGAC_28615 0.78 -2.1
5,493,658 + -0.3
5,493,691 - -2.6
5,493,691 - -3.5
5,493,691 - -1.0
5,493,817 - hisC JDDGAC_28620 0.11 -0.4
5,493,878 + hisC JDDGAC_28620 0.16 -2.7
5,493,879 - hisC JDDGAC_28620 0.16 -3.2
5,493,879 - hisC JDDGAC_28620 0.16 -2.9
5,494,214 + hisC JDDGAC_28620 0.48 +0.0
5,494,214 + hisC JDDGAC_28620 0.48 -0.7
5,494,214 + hisC JDDGAC_28620 0.48 +0.7
5,494,322 + hisC JDDGAC_28620 0.58 -2.6
5,494,323 - hisC JDDGAC_28620 0.58 -1.4
5,494,323 - hisC JDDGAC_28620 0.58 -3.3
5,494,363 + hisC JDDGAC_28620 0.62 -1.0
5,494,436 + hisC JDDGAC_28620 0.68 -1.6
5,494,437 - hisC JDDGAC_28620 0.69 -1.7
5,494,512 + hisC JDDGAC_28620 0.76 +0.2
5,494,555 + hisC JDDGAC_28620 0.80 -0.1
5,494,556 - hisC JDDGAC_28620 0.80 -3.8

Or see this region's nucleotide sequence