Experiment: Control_ECRC98_WM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzzB and hisIE are separated by 96 nucleotides hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides
JDDGAC_28590: wzzB - LPS O-antigen chain length determinant protein WzzB, at 5,488,872 to 5,489,849
wzzB
JDDGAC_28595: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 5,489,946 to 5,490,557
hisIE
JDDGAC_28600: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 5,490,551 to 5,491,327
hisF
JDDGAC_28605: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 5,491,309 to 5,492,046
hisA
Position (kb) 5489
5490
5491 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5489.090 kb on + strand, within wzzB at 5489.108 kb on + strand, within wzzB at 5489.108 kb on + strand, within wzzB at 5489.108 kb on + strand, within wzzB at 5489.112 kb on + strand, within wzzB at 5489.112 kb on + strand, within wzzB at 5489.246 kb on + strand, within wzzB at 5489.260 kb on + strand, within wzzB at 5489.260 kb on + strand, within wzzB at 5489.322 kb on + strand, within wzzB at 5489.431 kb on + strand, within wzzB at 5489.431 kb on + strand, within wzzB at 5489.431 kb on + strand, within wzzB at 5489.431 kb on + strand, within wzzB at 5489.460 kb on - strand, within wzzB at 5489.487 kb on + strand, within wzzB at 5489.549 kb on + strand, within wzzB at 5489.549 kb on + strand, within wzzB at 5489.549 kb on + strand, within wzzB at 5489.591 kb on + strand, within wzzB at 5489.710 kb on + strand, within wzzB at 5489.726 kb on - strand, within wzzB at 5489.729 kb on + strand, within wzzB at 5489.936 kb on - strand at 5489.985 kb on + strand at 5490.026 kb on - strand, within hisIE at 5490.070 kb on - strand, within hisIE at 5490.143 kb on - strand, within hisIE at 5490.301 kb on - strand, within hisIE at 5490.337 kb on - strand, within hisIE at 5490.387 kb on - strand, within hisIE at 5490.451 kb on + strand, within hisIE at 5490.608 kb on - strand at 5490.938 kb on - strand, within hisF at 5491.146 kb on - strand, within hisF at 5491.311 kb on - strand at 5491.364 kb on - strand at 5491.490 kb on - strand, within hisA at 5491.490 kb on - strand, within hisA
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC98_WM remove 5,489,090 + wzzB JDDGAC_28590 0.22 -3.6 5,489,108 + wzzB JDDGAC_28590 0.24 +0.1 5,489,108 + wzzB JDDGAC_28590 0.24 -1.2 5,489,108 + wzzB JDDGAC_28590 0.24 -0.9 5,489,112 + wzzB JDDGAC_28590 0.25 -1.8 5,489,112 + wzzB JDDGAC_28590 0.25 -0.8 5,489,246 + wzzB JDDGAC_28590 0.38 -0.6 5,489,260 + wzzB JDDGAC_28590 0.40 -0.1 5,489,260 + wzzB JDDGAC_28590 0.40 -1.3 5,489,322 + wzzB JDDGAC_28590 0.46 -1.4 5,489,431 + wzzB JDDGAC_28590 0.57 -0.5 5,489,431 + wzzB JDDGAC_28590 0.57 -0.8 5,489,431 + wzzB JDDGAC_28590 0.57 -3.3 5,489,431 + wzzB JDDGAC_28590 0.57 -1.2 5,489,460 - wzzB JDDGAC_28590 0.60 -0.3 5,489,487 + wzzB JDDGAC_28590 0.63 +0.1 5,489,549 + wzzB JDDGAC_28590 0.69 -2.7 5,489,549 + wzzB JDDGAC_28590 0.69 -1.7 5,489,549 + wzzB JDDGAC_28590 0.69 -2.2 5,489,591 + wzzB JDDGAC_28590 0.74 -1.7 5,489,710 + wzzB JDDGAC_28590 0.86 -0.2 5,489,726 - wzzB JDDGAC_28590 0.87 +0.5 5,489,729 + wzzB JDDGAC_28590 0.88 -1.1 5,489,936 - +1.4 5,489,985 + +0.0 5,490,026 - hisIE JDDGAC_28595 0.13 -1.7 5,490,070 - hisIE JDDGAC_28595 0.20 -3.2 5,490,143 - hisIE JDDGAC_28595 0.32 -0.5 5,490,301 - hisIE JDDGAC_28595 0.58 -1.8 5,490,337 - hisIE JDDGAC_28595 0.64 -3.2 5,490,387 - hisIE JDDGAC_28595 0.72 -2.0 5,490,451 + hisIE JDDGAC_28595 0.83 +0.7 5,490,608 - -4.5 5,490,938 - hisF JDDGAC_28600 0.50 -2.3 5,491,146 - hisF JDDGAC_28600 0.77 -2.2 5,491,311 - -2.7 5,491,364 - -2.7 5,491,490 - hisA JDDGAC_28605 0.25 -2.7 5,491,490 - hisA JDDGAC_28605 0.25 -2.1
Or see this region's nucleotide sequence