Strain Fitness in Escherichia coli ECRC98 around JDDGAC_19070

Experiment: Control_ECRC98_WM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntespB and cesD2 are separated by 6 nucleotidescesD2 and escF are separated by 35 nucleotidesescF and escG are separated by 5 nucleotidesescG and espF are separated by 84 nucleotides JDDGAC_19055: espB - type III secretion system LEE translocon pore-forming subunit EspB, at 3,626,028 to 3,626,966 espB JDDGAC_19060: cesD2 - LcrR family type III secretion system chaperone CesD2, at 3,626,973 to 3,627,380 cesD2 JDDGAC_19065: escF - type III secretion system LEE needle filament protein EscF, at 3,627,416 to 3,627,637 escF JDDGAC_19070: escG - type III secretion system LEE needle protein cochaperone EscG, at 3,627,643 to 3,627,921 escG JDDGAC_19075: espF - type III secretion system LEE effector EspF, at 3,628,006 to 3,628,752 espF Position (kb) 3627 3628Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3626.756 kb on - strand, within espBat 3626.787 kb on - strand, within espBat 3626.841 kb on + strand, within espBat 3626.843 kb on + strand, within espBat 3626.874 kb on - strandat 3626.894 kb on + strandat 3626.894 kb on + strandat 3626.896 kb on + strandat 3626.897 kb on - strandat 3626.937 kb on - strandat 3626.956 kb on + strandat 3626.957 kb on - strandat 3626.988 kb on - strandat 3627.002 kb on + strandat 3627.002 kb on + strandat 3627.002 kb on + strandat 3627.003 kb on - strandat 3627.027 kb on + strand, within cesD2at 3627.034 kb on + strand, within cesD2at 3627.035 kb on - strand, within cesD2at 3627.171 kb on - strand, within cesD2at 3627.306 kb on + strand, within cesD2at 3627.306 kb on + strand, within cesD2at 3627.306 kb on + strand, within cesD2at 3627.306 kb on + strand, within cesD2at 3627.458 kb on - strand, within escFat 3627.667 kb on - strandat 3627.706 kb on - strand, within escGat 3627.874 kb on + strand, within escGat 3627.984 kb on + strandat 3627.990 kb on + strandat 3627.991 kb on - strandat 3628.005 kb on + strandat 3628.020 kb on + strandat 3628.021 kb on - strandat 3628.035 kb on + strandat 3628.036 kb on - strandat 3628.036 kb on - strandat 3628.041 kb on - strandat 3628.055 kb on + strandat 3628.056 kb on - strandat 3628.056 kb on - strandat 3628.060 kb on - strandat 3628.174 kb on - strand, within espFat 3628.191 kb on + strand, within espFat 3628.359 kb on + strand, within espFat 3628.359 kb on + strand, within espFat 3628.709 kb on + strandat 3628.709 kb on + strandat 3628.709 kb on + strandat 3628.722 kb on - strandat 3628.799 kb on + strandat 3628.799 kb on + strandat 3628.799 kb on + strandat 3628.800 kb on - strandat 3628.802 kb on + strandat 3628.838 kb on + strandat 3628.894 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC98_WM
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3,626,756 - espB JDDGAC_19055 0.78 -1.3
3,626,787 - espB JDDGAC_19055 0.81 +0.3
3,626,841 + espB JDDGAC_19055 0.87 +1.1
3,626,843 + espB JDDGAC_19055 0.87 +0.2
3,626,874 - +0.2
3,626,894 + -1.9
3,626,894 + -2.1
3,626,896 + -0.8
3,626,897 - +0.1
3,626,937 - -0.6
3,626,956 + +0.1
3,626,957 - -1.2
3,626,988 - -2.2
3,627,002 + +1.9
3,627,002 + -0.3
3,627,002 + -0.8
3,627,003 - -1.3
3,627,027 + cesD2 JDDGAC_19060 0.13 +0.2
3,627,034 + cesD2 JDDGAC_19060 0.15 -0.1
3,627,035 - cesD2 JDDGAC_19060 0.15 +0.5
3,627,171 - cesD2 JDDGAC_19060 0.49 +0.4
3,627,306 + cesD2 JDDGAC_19060 0.82 +1.2
3,627,306 + cesD2 JDDGAC_19060 0.82 -1.0
3,627,306 + cesD2 JDDGAC_19060 0.82 +0.1
3,627,306 + cesD2 JDDGAC_19060 0.82 -2.3
3,627,458 - escF JDDGAC_19065 0.19 +1.2
3,627,667 - +0.1
3,627,706 - escG JDDGAC_19070 0.23 -1.9
3,627,874 + escG JDDGAC_19070 0.83 -2.0
3,627,984 + -0.3
3,627,990 + -1.1
3,627,991 - -1.7
3,628,005 + -0.8
3,628,020 + +0.2
3,628,021 - +0.8
3,628,035 + +1.8
3,628,036 - +0.5
3,628,036 - +0.3
3,628,041 - -3.0
3,628,055 + +0.1
3,628,056 - -0.8
3,628,056 - -0.1
3,628,060 - -0.2
3,628,174 - espF JDDGAC_19075 0.22 +0.8
3,628,191 + espF JDDGAC_19075 0.25 -1.9
3,628,359 + espF JDDGAC_19075 0.47 +1.2
3,628,359 + espF JDDGAC_19075 0.47 +1.6
3,628,709 + +0.6
3,628,709 + -1.0
3,628,709 + -0.3
3,628,722 - +0.6
3,628,799 + +1.9
3,628,799 + +1.8
3,628,799 + +2.4
3,628,800 - +1.2
3,628,802 + +0.2
3,628,838 + +0.7
3,628,894 + +1.3

Or see this region's nucleotide sequence