Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28520

Experiment: WM_Bas41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgalF and wcaA are separated by 318 nucleotideswcaA and wzy overlap by 4 nucleotides JDDGAC_28515: galF - UTP--glucose-1-phosphate uridylyltransferase GalF, at 5,470,750 to 5,471,643 galF JDDGAC_28520: wcaA - glycosyl transferase, at 5,471,962 to 5,472,744 wcaA JDDGAC_28525: wzy - O157 family O-antigen polymerase, at 5,472,741 to 5,473,925 wzy Position (kb) 5471 5472 5473Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5470.967 kb on + strand, within galFat 5471.003 kb on + strand, within galFat 5471.015 kb on + strand, within galFat 5471.022 kb on + strand, within galFat 5471.046 kb on + strand, within galFat 5471.073 kb on - strand, within galFat 5471.093 kb on + strand, within galFat 5471.247 kb on + strand, within galFat 5471.430 kb on + strand, within galFat 5471.430 kb on + strand, within galFat 5471.454 kb on + strand, within galFat 5471.530 kb on + strand, within galFat 5471.554 kb on + strandat 5471.556 kb on + strandat 5471.556 kb on + strandat 5471.575 kb on + strandat 5471.582 kb on + strandat 5472.302 kb on + strand, within wcaAat 5472.635 kb on + strand, within wcaAat 5472.676 kb on + strandat 5472.786 kb on + strandat 5473.094 kb on + strand, within wzyat 5473.120 kb on + strand, within wzyat 5473.336 kb on + strand, within wzyat 5473.336 kb on + strand, within wzy

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas41
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5,470,967 + galF JDDGAC_28515 0.24 +0.0
5,471,003 + galF JDDGAC_28515 0.28 -0.2
5,471,015 + galF JDDGAC_28515 0.30 -2.1
5,471,022 + galF JDDGAC_28515 0.30 -2.1
5,471,046 + galF JDDGAC_28515 0.33 -2.1
5,471,073 - galF JDDGAC_28515 0.36 +0.3
5,471,093 + galF JDDGAC_28515 0.38 -2.3
5,471,247 + galF JDDGAC_28515 0.56 -1.9
5,471,430 + galF JDDGAC_28515 0.76 -0.6
5,471,430 + galF JDDGAC_28515 0.76 -0.7
5,471,454 + galF JDDGAC_28515 0.79 -3.3
5,471,530 + galF JDDGAC_28515 0.87 +2.4
5,471,554 + +0.1
5,471,556 + +0.3
5,471,556 + +1.4
5,471,575 + +0.3
5,471,582 + -0.1
5,472,302 + wcaA JDDGAC_28520 0.43 -2.9
5,472,635 + wcaA JDDGAC_28520 0.86 -2.6
5,472,676 + -1.7
5,472,786 + -2.1
5,473,094 + wzy JDDGAC_28525 0.30 +0.3
5,473,120 + wzy JDDGAC_28525 0.32 -1.5
5,473,336 + wzy JDDGAC_28525 0.50 -4.0
5,473,336 + wzy JDDGAC_28525 0.50 -2.1

Or see this region's nucleotide sequence