Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltX and tnpA are separated by 0 nucleotides tnpA and JDDGAC_26215 are separated by 67 nucleotides JDDGAC_26215 and tnpB are separated by 75 nucleotides tnpB and tnpB overlap by 4 nucleotides tnpB and JDDGAC_26230 are separated by 49 nucleotides
JDDGAC_26205: gltX - glutamate--tRNA ligase, at 5,010,524 to 5,011,939
gltX
JDDGAC_26210: tnpA - IS200/IS605 family transposase, at 5,011,940 to 5,012,482
tnpA
JDDGAC_26215: JDDGAC_26215 - RNA-guided endonuclease TnpB family protein, at 5,012,550 to 5,013,374
_26215
JDDGAC_26220: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 5,013,450 to 5,013,830
tnpB
JDDGAC_26225: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 5,013,827 to 5,014,174
tnpB
JDDGAC_26230: JDDGAC_26230 - IS66-like element ISEc8 family transposase, at 5,014,224 to 5,015,762
_26230
Position (kb)
5012
5013
5014 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5011.937 kb on + strand at 5011.937 kb on + strand at 5011.948 kb on + strand at 5012.031 kb on + strand, within tnpA at 5012.033 kb on + strand, within tnpA at 5012.033 kb on + strand, within tnpA at 5012.033 kb on + strand, within tnpA at 5012.996 kb on - strand, within JDDGAC_26215 at 5013.143 kb on - strand, within JDDGAC_26215
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 5,011,937 + +1.2 5,011,937 + +2.1 5,011,948 + -0.2 5,012,031 + tnpA JDDGAC_26210 0.17 +0.0 5,012,033 + tnpA JDDGAC_26210 0.17 -0.3 5,012,033 + tnpA JDDGAC_26210 0.17 -0.3 5,012,033 + tnpA JDDGAC_26210 0.17 +0.6 5,012,996 - JDDGAC_26215 0.54 +2.4 5,013,143 - JDDGAC_26215 0.72 -2.6
Or see this region's nucleotide sequence