Strain Fitness in Escherichia coli ECRC98 around JDDGAC_26215

Experiment: WM_Bas41

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgltX and tnpA are separated by 0 nucleotidestnpA and JDDGAC_26215 are separated by 67 nucleotidesJDDGAC_26215 and tnpB are separated by 75 nucleotidestnpB and tnpB overlap by 4 nucleotidestnpB and JDDGAC_26230 are separated by 49 nucleotides JDDGAC_26205: gltX - glutamate--tRNA ligase, at 5,010,524 to 5,011,939 gltX JDDGAC_26210: tnpA - IS200/IS605 family transposase, at 5,011,940 to 5,012,482 tnpA JDDGAC_26215: JDDGAC_26215 - RNA-guided endonuclease TnpB family protein, at 5,012,550 to 5,013,374 _26215 JDDGAC_26220: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 5,013,450 to 5,013,830 tnpB JDDGAC_26225: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 5,013,827 to 5,014,174 tnpB JDDGAC_26230: JDDGAC_26230 - IS66-like element ISEc8 family transposase, at 5,014,224 to 5,015,762 _26230 Position (kb) 5012 5013 5014Strain fitness (log2 ratio) -2 -1 0 1 2at 5011.937 kb on + strandat 5011.937 kb on + strandat 5011.948 kb on + strandat 5012.031 kb on + strand, within tnpAat 5012.033 kb on + strand, within tnpAat 5012.033 kb on + strand, within tnpAat 5012.033 kb on + strand, within tnpAat 5012.996 kb on - strand, within JDDGAC_26215at 5013.143 kb on - strand, within JDDGAC_26215

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas41
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5,011,937 + +1.2
5,011,937 + +2.1
5,011,948 + -0.2
5,012,031 + tnpA JDDGAC_26210 0.17 +0.0
5,012,033 + tnpA JDDGAC_26210 0.17 -0.3
5,012,033 + tnpA JDDGAC_26210 0.17 -0.3
5,012,033 + tnpA JDDGAC_26210 0.17 +0.6
5,012,996 - JDDGAC_26215 0.54 +2.4
5,013,143 - JDDGAC_26215 0.72 -2.6

Or see this region's nucleotide sequence