Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysI and cysH are separated by 73 nucleotides cysH and hokG are separated by 263 nucleotides hokG and cas3 are separated by 191 nucleotides
JDDGAC_24040: cysI - assimilatory sulfite reductase (NADPH) hemoprotein subunit, at 4,610,211 to 4,611,923
cysI
JDDGAC_24045: cysH - phosphoadenosine phosphosulfate reductase, at 4,611,997 to 4,612,731
cysH
JDDGAC_24055: hokG - Putative protein HokG, at 4,612,995 to 4,613,147
hokG
JDDGAC_24060: cas3 - CRISPR-associated helicase/endonuclease Cas3, at 4,613,339 to 4,616,038
cas3
Position (kb)
4611
4612
4613 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4611.009 kb on + strand, within cysI at 4611.053 kb on + strand, within cysI at 4611.054 kb on - strand, within cysI at 4611.219 kb on + strand, within cysI at 4611.245 kb on + strand, within cysI at 4611.592 kb on + strand, within cysI at 4611.624 kb on - strand, within cysI at 4611.637 kb on + strand, within cysI at 4611.814 kb on + strand at 4611.942 kb on - strand at 4611.950 kb on + strand at 4611.996 kb on + strand at 4611.997 kb on - strand at 4611.997 kb on - strand at 4612.107 kb on - strand, within cysH at 4612.139 kb on - strand, within cysH at 4612.139 kb on - strand, within cysH at 4612.139 kb on - strand, within cysH at 4612.142 kb on + strand, within cysH at 4612.143 kb on - strand, within cysH at 4612.143 kb on - strand, within cysH at 4612.143 kb on - strand, within cysH at 4612.438 kb on - strand, within cysH at 4612.566 kb on - strand, within cysH at 4612.566 kb on - strand, within cysH at 4612.567 kb on + strand, within cysH at 4612.568 kb on - strand, within cysH at 4612.573 kb on + strand, within cysH at 4612.574 kb on - strand, within cysH at 4612.644 kb on + strand, within cysH at 4612.645 kb on - strand, within cysH at 4612.730 kb on - strand at 4612.841 kb on - strand at 4612.842 kb on + strand at 4612.843 kb on - strand at 4612.843 kb on - strand at 4612.919 kb on + strand at 4612.926 kb on - strand at 4612.926 kb on - strand at 4612.952 kb on + strand at 4613.008 kb on - strand at 4613.008 kb on - strand at 4613.026 kb on + strand, within hokG at 4613.026 kb on + strand, within hokG at 4613.026 kb on + strand, within hokG at 4613.026 kb on + strand, within hokG at 4613.026 kb on + strand, within hokG at 4613.027 kb on - strand, within hokG at 4613.027 kb on - strand, within hokG at 4613.029 kb on + strand, within hokG at 4613.030 kb on - strand, within hokG at 4613.030 kb on - strand, within hokG at 4613.030 kb on - strand, within hokG at 4613.030 kb on - strand, within hokG at 4613.039 kb on + strand, within hokG at 4613.040 kb on - strand, within hokG at 4613.044 kb on + strand, within hokG at 4613.044 kb on + strand, within hokG at 4613.090 kb on + strand, within hokG at 4613.105 kb on + strand, within hokG at 4613.115 kb on - strand, within hokG at 4613.131 kb on - strand, within hokG at 4613.145 kb on + strand at 4613.146 kb on - strand at 4613.338 kb on + strand at 4613.486 kb on + strand at 4613.490 kb on + strand at 4613.538 kb on + strand at 4613.633 kb on + strand, within cas3 at 4613.679 kb on - strand, within cas3 at 4613.726 kb on + strand, within cas3 at 4613.726 kb on + strand, within cas3 at 4613.727 kb on - strand, within cas3 at 4613.730 kb on + strand, within cas3
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 4,611,009 + cysI JDDGAC_24040 0.47 -0.7 4,611,053 + cysI JDDGAC_24040 0.49 -1.0 4,611,054 - cysI JDDGAC_24040 0.49 +0.4 4,611,219 + cysI JDDGAC_24040 0.59 -0.5 4,611,245 + cysI JDDGAC_24040 0.60 -2.6 4,611,592 + cysI JDDGAC_24040 0.81 +0.8 4,611,624 - cysI JDDGAC_24040 0.82 +1.5 4,611,637 + cysI JDDGAC_24040 0.83 -0.9 4,611,814 + +0.3 4,611,942 - -1.6 4,611,950 + -0.2 4,611,996 + -0.7 4,611,997 - +0.7 4,611,997 - +1.6 4,612,107 - cysH JDDGAC_24045 0.15 -0.3 4,612,139 - cysH JDDGAC_24045 0.19 -1.4 4,612,139 - cysH JDDGAC_24045 0.19 -0.9 4,612,139 - cysH JDDGAC_24045 0.19 -1.3 4,612,142 + cysH JDDGAC_24045 0.20 -2.2 4,612,143 - cysH JDDGAC_24045 0.20 -1.6 4,612,143 - cysH JDDGAC_24045 0.20 -0.5 4,612,143 - cysH JDDGAC_24045 0.20 -1.7 4,612,438 - cysH JDDGAC_24045 0.60 +0.3 4,612,566 - cysH JDDGAC_24045 0.77 -1.7 4,612,566 - cysH JDDGAC_24045 0.77 -2.8 4,612,567 + cysH JDDGAC_24045 0.78 -1.0 4,612,568 - cysH JDDGAC_24045 0.78 -1.9 4,612,573 + cysH JDDGAC_24045 0.78 +0.0 4,612,574 - cysH JDDGAC_24045 0.79 -1.0 4,612,644 + cysH JDDGAC_24045 0.88 -0.8 4,612,645 - cysH JDDGAC_24045 0.88 -1.1 4,612,730 - +0.6 4,612,841 - +1.4 4,612,842 + +2.9 4,612,843 - +0.6 4,612,843 - +1.7 4,612,919 + -1.8 4,612,926 - +0.2 4,612,926 - +0.9 4,612,952 + -0.8 4,613,008 - -0.0 4,613,008 - -1.6 4,613,026 + hokG JDDGAC_24055 0.20 +0.2 4,613,026 + hokG JDDGAC_24055 0.20 +0.3 4,613,026 + hokG JDDGAC_24055 0.20 +0.7 4,613,026 + hokG JDDGAC_24055 0.20 +0.7 4,613,026 + hokG JDDGAC_24055 0.20 -0.1 4,613,027 - hokG JDDGAC_24055 0.21 +0.0 4,613,027 - hokG JDDGAC_24055 0.21 -0.7 4,613,029 + hokG JDDGAC_24055 0.22 +1.5 4,613,030 - hokG JDDGAC_24055 0.23 +0.7 4,613,030 - hokG JDDGAC_24055 0.23 -0.3 4,613,030 - hokG JDDGAC_24055 0.23 -0.7 4,613,030 - hokG JDDGAC_24055 0.23 +0.2 4,613,039 + hokG JDDGAC_24055 0.29 -0.7 4,613,040 - hokG JDDGAC_24055 0.29 -0.2 4,613,044 + hokG JDDGAC_24055 0.32 +0.1 4,613,044 + hokG JDDGAC_24055 0.32 +0.5 4,613,090 + hokG JDDGAC_24055 0.62 -0.8 4,613,105 + hokG JDDGAC_24055 0.72 -0.9 4,613,115 - hokG JDDGAC_24055 0.78 -1.2 4,613,131 - hokG JDDGAC_24055 0.89 -2.3 4,613,145 + +0.0 4,613,146 - -0.2 4,613,338 + +0.1 4,613,486 + -0.2 4,613,490 + +0.1 4,613,538 + -0.7 4,613,633 + cas3 JDDGAC_24060 0.11 -2.9 4,613,679 - cas3 JDDGAC_24060 0.13 -2.7 4,613,726 + cas3 JDDGAC_24060 0.14 -1.1 4,613,726 + cas3 JDDGAC_24060 0.14 +0.5 4,613,727 - cas3 JDDGAC_24060 0.14 -1.8 4,613,730 + cas3 JDDGAC_24060 0.14 +0.9
Or see this region's nucleotide sequence