Experiment: WM_Bas41
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lapB and lapA are separated by 6 nucleotides lapA and pgpB are separated by 148 nucleotides pgpB and ribA are separated by 169 nucleotides ribA and acnA are separated by 63 nucleotides
JDDGAC_05100: lapB - lipopolysaccharide assembly protein LapB, at 895,475 to 896,644
lapB
JDDGAC_05105: lapA - lipopolysaccharide assembly protein LapA, at 896,651 to 896,959
lapA
JDDGAC_05110: pgpB - phosphatidylglycerophosphatase B, at 897,108 to 897,872
pgpB
JDDGAC_05115: ribA - GTP cyclohydrolase-2, at 898,042 to 898,632
ribA
JDDGAC_05120: acnA - aconitate hydratase AcnA, at 898,696 to 901,371
acnA
Position (kb) 897
898 Strain fitness (log2 ratio) -2
-1
0
1
2 at 896.840 kb on - strand, within lapA at 896.870 kb on - strand, within lapA at 896.870 kb on - strand, within lapA at 896.963 kb on - strand at 896.990 kb on - strand at 897.027 kb on - strand at 897.163 kb on - strand at 897.227 kb on + strand, within pgpB at 897.228 kb on - strand, within pgpB at 897.309 kb on - strand, within pgpB at 897.528 kb on - strand, within pgpB at 897.643 kb on - strand, within pgpB at 897.708 kb on - strand, within pgpB at 897.759 kb on + strand, within pgpB at 897.760 kb on - strand, within pgpB at 897.821 kb on + strand at 897.831 kb on + strand at 898.005 kb on + strand at 898.005 kb on + strand at 898.005 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas41 remove 896,840 - lapA JDDGAC_05105 0.61 +1.2 896,870 - lapA JDDGAC_05105 0.71 +1.1 896,870 - lapA JDDGAC_05105 0.71 +0.2 896,963 - -2.3 896,990 - +0.1 897,027 - +2.3 897,163 - +0.3 897,227 + pgpB JDDGAC_05110 0.16 -0.3 897,228 - pgpB JDDGAC_05110 0.16 -1.2 897,309 - pgpB JDDGAC_05110 0.26 -1.3 897,528 - pgpB JDDGAC_05110 0.55 -1.0 897,643 - pgpB JDDGAC_05110 0.70 -1.3 897,708 - pgpB JDDGAC_05110 0.78 -0.5 897,759 + pgpB JDDGAC_05110 0.85 +1.1 897,760 - pgpB JDDGAC_05110 0.85 -1.0 897,821 + -1.1 897,831 + +0.8 898,005 + +0.3 898,005 + -2.3 898,005 + +0.9
Or see this region's nucleotide sequence