Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28550

Experiment: WM_TP3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaB and JDDGAC_28550 are separated by 19 nucleotidesJDDGAC_28550 and wcaG are separated by 2 nucleotideswcaG and wbdQ are separated by 2 nucleotides JDDGAC_28545: rfaB - glycosyl transferase, at 5,477,140 to 5,478,354 rfaB JDDGAC_28550: JDDGAC_28550 - GDP-mannose 4,6-dehydratase, at 5,478,374 to 5,479,492 _28550 JDDGAC_28555: wcaG - GDP-fucose synthetase, at 5,479,495 to 5,480,460 wcaG JDDGAC_28560: wbdQ - GDP-mannose mannosyl hydrolase WbdQ/WbhG, at 5,480,463 to 5,480,972 wbdQ Position (kb) 5478 5479 5480Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5478.146 kb on + strand, within rfaBat 5478.860 kb on + strand, within JDDGAC_28550at 5479.304 kb on + strand, within JDDGAC_28550at 5480.314 kb on - strand, within wcaGat 5480.470 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP3
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5,478,146 + rfaB JDDGAC_28545 0.83 -1.7
5,478,860 + JDDGAC_28550 0.43 +0.9
5,479,304 + JDDGAC_28550 0.83 -0.8
5,480,314 - wcaG JDDGAC_28555 0.85 -1.7
5,480,470 + -3.8

Or see this region's nucleotide sequence