Experiment: Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potB and espK are separated by 363 nucleotides espK and JDDGAC_06755 are separated by 188 nucleotides JDDGAC_06755 and JDDGAC_06760 are separated by 141 nucleotides JDDGAC_06760 and tnp-IS3 are separated by 10 nucleotides
JDDGAC_06745: potB - spermidine/putrescine ABC transporter permease PotB, at 1,174,175 to 1,175,038
potB
JDDGAC_06750: espK - type III secretion system effector EspK, at 1,175,402 to 1,176,763
espK
JDDGAC_06755: JDDGAC_06755 - hypothetical protein, at 1,176,952 to 1,177,185
_06755
JDDGAC_06760: JDDGAC_06760 - Mutator family transposase, at 1,177,327 to 1,177,626
_06760
JDDGAC_06765: tnp-IS3 - IS3 family transposase, at 1,177,637 to 1,177,798
tnp-IS3
Position (kb)
1175
1176
1177 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1174.527 kb on - strand, within potB at 1174.559 kb on + strand, within potB at 1174.683 kb on - strand, within potB at 1174.771 kb on + strand, within potB at 1174.841 kb on + strand, within potB at 1174.841 kb on + strand, within potB at 1174.899 kb on + strand, within potB at 1174.962 kb on + strand at 1175.286 kb on + strand at 1175.350 kb on + strand at 1175.359 kb on + strand at 1175.367 kb on - strand at 1175.529 kb on - strand at 1175.621 kb on - strand, within espK at 1175.635 kb on + strand, within espK at 1175.786 kb on + strand, within espK at 1175.786 kb on + strand, within espK at 1175.790 kb on + strand, within espK at 1175.895 kb on - strand, within espK at 1175.975 kb on - strand, within espK at 1175.977 kb on + strand, within espK at 1175.977 kb on + strand, within espK at 1175.978 kb on - strand, within espK at 1176.118 kb on - strand, within espK at 1176.194 kb on + strand, within espK at 1176.562 kb on + strand, within espK at 1176.562 kb on + strand, within espK at 1176.921 kb on + strand at 1176.926 kb on - strand at 1176.946 kb on - strand at 1176.949 kb on + strand at 1176.953 kb on + strand at 1177.056 kb on + strand, within JDDGAC_06755 at 1177.081 kb on - strand, within JDDGAC_06755 at 1177.215 kb on - strand at 1177.261 kb on + strand at 1177.538 kb on + strand, within JDDGAC_06760 at 1177.589 kb on + strand, within JDDGAC_06760 at 1177.603 kb on - strand at 1177.603 kb on - strand at 1177.605 kb on + strand at 1177.615 kb on + strand at 1177.620 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lambda remove 1,174,527 - potB JDDGAC_06745 0.41 -1.2 1,174,559 + potB JDDGAC_06745 0.44 +0.1 1,174,683 - potB JDDGAC_06745 0.59 -1.1 1,174,771 + potB JDDGAC_06745 0.69 -0.5 1,174,841 + potB JDDGAC_06745 0.77 -0.8 1,174,841 + potB JDDGAC_06745 0.77 +1.4 1,174,899 + potB JDDGAC_06745 0.84 +0.9 1,174,962 + -1.4 1,175,286 + -0.9 1,175,350 + -0.4 1,175,359 + +1.7 1,175,367 - -1.8 1,175,529 - +2.0 1,175,621 - espK JDDGAC_06750 0.16 +0.8 1,175,635 + espK JDDGAC_06750 0.17 +1.4 1,175,786 + espK JDDGAC_06750 0.28 +1.0 1,175,786 + espK JDDGAC_06750 0.28 +1.2 1,175,790 + espK JDDGAC_06750 0.28 +0.7 1,175,895 - espK JDDGAC_06750 0.36 +0.6 1,175,975 - espK JDDGAC_06750 0.42 -1.0 1,175,977 + espK JDDGAC_06750 0.42 +0.4 1,175,977 + espK JDDGAC_06750 0.42 +1.2 1,175,978 - espK JDDGAC_06750 0.42 +5.5 1,176,118 - espK JDDGAC_06750 0.53 -0.2 1,176,194 + espK JDDGAC_06750 0.58 -1.0 1,176,562 + espK JDDGAC_06750 0.85 -0.3 1,176,562 + espK JDDGAC_06750 0.85 -0.3 1,176,921 + -0.6 1,176,926 - -1.5 1,176,946 - -0.7 1,176,949 + -0.6 1,176,953 + +0.1 1,177,056 + JDDGAC_06755 0.44 +0.1 1,177,081 - JDDGAC_06755 0.55 -0.4 1,177,215 - +0.7 1,177,261 + +1.8 1,177,538 + JDDGAC_06760 0.70 +1.5 1,177,589 + JDDGAC_06760 0.87 +0.4 1,177,603 - +0.8 1,177,603 - +1.6 1,177,605 + +1.1 1,177,615 + +0.2 1,177,620 + -0.5
Or see this region's nucleotide sequence