Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt stxB2c and iraM are separated by 296 nucleotides iraM and JDDGAC_28935 are separated by 243 nucleotides JDDGAC_28935 and JDDGAC_28940 are separated by 136 nucleotides JDDGAC_28940 and JDDGAC_28945 are separated by 40 nucleotides JDDGAC_28945 and JDDGAC_28950 are separated by 76 nucleotides JDDGAC_28950 and rrrD overlap by 1 nucleotides
JDDGAC_28920: stxB2c - Shiga toxin Stx2c subunit B, at 5,526,495 to 5,526,764
stxB2c
JDDGAC_28930: iraM - Anti-adapter protein IraM, at 5,527,061 to 5,527,384
iraM
JDDGAC_28935: JDDGAC_28935 - sialate O-acetylesterase, at 5,527,628 to 5,529,565
_28935
JDDGAC_28940: JDDGAC_28940 - DUF1378 domain-containing protein, at 5,529,702 to 5,529,881
_28940
JDDGAC_28945: JDDGAC_28945 - DUF826 domain-containing protein, at 5,529,922 to 5,530,194
_28945
JDDGAC_28950: JDDGAC_28950 - holin, at 5,530,271 to 5,530,486
_28950
JDDGAC_28955: rrrD - lysozyme RrrD, at 5,530,486 to 5,530,983
rrrD
Position (kb)
5527
5528
5529
5530 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5526.752 kb on + strand at 5526.906 kb on + strand at 5526.907 kb on - strand at 5526.941 kb on + strand at 5526.995 kb on + strand at 5526.996 kb on - strand at 5526.996 kb on - strand at 5526.996 kb on - strand at 5526.996 kb on - strand at 5527.011 kb on + strand at 5527.046 kb on - strand at 5527.083 kb on - strand at 5527.105 kb on + strand, within iraM at 5527.164 kb on - strand, within iraM at 5527.180 kb on + strand, within iraM at 5527.236 kb on - strand, within iraM at 5527.537 kb on + strand at 5527.578 kb on + strand at 5527.578 kb on + strand at 5527.603 kb on + strand at 5527.637 kb on - strand at 5527.637 kb on - strand at 5527.856 kb on + strand, within JDDGAC_28935 at 5527.859 kb on + strand, within JDDGAC_28935 at 5527.859 kb on + strand, within JDDGAC_28935 at 5527.932 kb on - strand, within JDDGAC_28935 at 5527.954 kb on + strand, within JDDGAC_28935 at 5528.009 kb on - strand, within JDDGAC_28935 at 5530.193 kb on - strand at 5530.459 kb on + strand, within JDDGAC_28950 at 5530.484 kb on + strand at 5530.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 5,526,752 + -0.3 5,526,906 + -0.0 5,526,907 - +1.3 5,526,941 + +0.2 5,526,995 + -1.6 5,526,996 - -0.6 5,526,996 - +1.8 5,526,996 - -0.2 5,526,996 - +1.1 5,527,011 + -2.2 5,527,046 - -0.5 5,527,083 - -0.4 5,527,105 + iraM JDDGAC_28930 0.14 -1.1 5,527,164 - iraM JDDGAC_28930 0.32 +0.5 5,527,180 + iraM JDDGAC_28930 0.37 +0.8 5,527,236 - iraM JDDGAC_28930 0.54 -1.5 5,527,537 + -0.6 5,527,578 + -0.3 5,527,578 + -0.2 5,527,603 + +0.4 5,527,637 - +0.4 5,527,637 - -0.5 5,527,856 + JDDGAC_28935 0.12 -0.5 5,527,859 + JDDGAC_28935 0.12 -0.4 5,527,859 + JDDGAC_28935 0.12 -2.7 5,527,932 - JDDGAC_28935 0.16 +0.6 5,527,954 + JDDGAC_28935 0.17 -1.4 5,528,009 - JDDGAC_28935 0.20 -0.4 5,530,193 - -1.0 5,530,459 + JDDGAC_28950 0.87 -0.5 5,530,484 + -0.2 5,530,522 - +1.0
Or see this region's nucleotide sequence