Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28935

Experiment: WM_Lambda

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntstxB2c and iraM are separated by 296 nucleotidesiraM and JDDGAC_28935 are separated by 243 nucleotidesJDDGAC_28935 and JDDGAC_28940 are separated by 136 nucleotidesJDDGAC_28940 and JDDGAC_28945 are separated by 40 nucleotidesJDDGAC_28945 and JDDGAC_28950 are separated by 76 nucleotidesJDDGAC_28950 and rrrD overlap by 1 nucleotides JDDGAC_28920: stxB2c - Shiga toxin Stx2c subunit B, at 5,526,495 to 5,526,764 stxB2c JDDGAC_28930: iraM - Anti-adapter protein IraM, at 5,527,061 to 5,527,384 iraM JDDGAC_28935: JDDGAC_28935 - sialate O-acetylesterase, at 5,527,628 to 5,529,565 _28935 JDDGAC_28940: JDDGAC_28940 - DUF1378 domain-containing protein, at 5,529,702 to 5,529,881 _28940 JDDGAC_28945: JDDGAC_28945 - DUF826 domain-containing protein, at 5,529,922 to 5,530,194 _28945 JDDGAC_28950: JDDGAC_28950 - holin, at 5,530,271 to 5,530,486 _28950 JDDGAC_28955: rrrD - lysozyme RrrD, at 5,530,486 to 5,530,983 rrrD Position (kb) 5527 5528 5529 5530Strain fitness (log2 ratio) -2 -1 0 1 2at 5526.752 kb on + strandat 5526.906 kb on + strandat 5526.907 kb on - strandat 5526.941 kb on + strandat 5526.995 kb on + strandat 5526.996 kb on - strandat 5526.996 kb on - strandat 5526.996 kb on - strandat 5526.996 kb on - strandat 5527.011 kb on + strandat 5527.046 kb on - strandat 5527.083 kb on - strandat 5527.105 kb on + strand, within iraMat 5527.164 kb on - strand, within iraMat 5527.180 kb on + strand, within iraMat 5527.236 kb on - strand, within iraMat 5527.537 kb on + strandat 5527.578 kb on + strandat 5527.578 kb on + strandat 5527.603 kb on + strandat 5527.637 kb on - strandat 5527.637 kb on - strandat 5527.856 kb on + strand, within JDDGAC_28935at 5527.859 kb on + strand, within JDDGAC_28935at 5527.859 kb on + strand, within JDDGAC_28935at 5527.932 kb on - strand, within JDDGAC_28935at 5527.954 kb on + strand, within JDDGAC_28935at 5528.009 kb on - strand, within JDDGAC_28935at 5530.193 kb on - strandat 5530.459 kb on + strand, within JDDGAC_28950at 5530.484 kb on + strandat 5530.522 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Lambda
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5,526,752 + -0.3
5,526,906 + -0.0
5,526,907 - +1.3
5,526,941 + +0.2
5,526,995 + -1.6
5,526,996 - -0.6
5,526,996 - +1.8
5,526,996 - -0.2
5,526,996 - +1.1
5,527,011 + -2.2
5,527,046 - -0.5
5,527,083 - -0.4
5,527,105 + iraM JDDGAC_28930 0.14 -1.1
5,527,164 - iraM JDDGAC_28930 0.32 +0.5
5,527,180 + iraM JDDGAC_28930 0.37 +0.8
5,527,236 - iraM JDDGAC_28930 0.54 -1.5
5,527,537 + -0.6
5,527,578 + -0.3
5,527,578 + -0.2
5,527,603 + +0.4
5,527,637 - +0.4
5,527,637 - -0.5
5,527,856 + JDDGAC_28935 0.12 -0.5
5,527,859 + JDDGAC_28935 0.12 -0.4
5,527,859 + JDDGAC_28935 0.12 -2.7
5,527,932 - JDDGAC_28935 0.16 +0.6
5,527,954 + JDDGAC_28935 0.17 -1.4
5,528,009 - JDDGAC_28935 0.20 -0.4
5,530,193 - -1.0
5,530,459 + JDDGAC_28950 0.87 -0.5
5,530,484 + -0.2
5,530,522 - +1.0

Or see this region's nucleotide sequence