Strain Fitness in Escherichia coli ECRC98 around JDDGAC_27415

Experiment: WM_Lambda

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpsuK and psuG overlap by 13 nucleotidespsuG and psuT are separated by 93 nucleotides JDDGAC_27410: psuK - pseudouridine kinase, at 5,266,734 to 5,267,675 psuK JDDGAC_27415: psuG - pseudouridine-5'-phosphate glycosidase, at 5,267,663 to 5,268,601 psuG JDDGAC_27420: psuT - Putative pseudouridine transporter, at 5,268,695 to 5,269,945 psuT Position (kb) 5267 5268 5269Strain fitness (log2 ratio) -2 -1 0 1 2at 5266.679 kb on + strandat 5266.684 kb on - strandat 5267.023 kb on - strand, within psuKat 5267.864 kb on - strand, within psuGat 5268.037 kb on + strand, within psuGat 5268.043 kb on - strand, within psuGat 5268.192 kb on - strand, within psuGat 5268.403 kb on + strand, within psuGat 5268.404 kb on - strand, within psuGat 5268.643 kb on + strandat 5268.643 kb on + strandat 5268.702 kb on + strandat 5268.702 kb on + strandat 5268.703 kb on - strandat 5268.742 kb on - strandat 5268.861 kb on + strand, within psuTat 5268.899 kb on + strand, within psuTat 5268.900 kb on - strand, within psuTat 5269.043 kb on + strand, within psuTat 5269.043 kb on + strand, within psuTat 5269.043 kb on + strand, within psuTat 5269.222 kb on + strand, within psuTat 5269.348 kb on + strand, within psuTat 5269.348 kb on + strand, within psuTat 5269.349 kb on - strand, within psuTat 5269.511 kb on - strand, within psuT

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Lambda
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5,266,679 + +0.3
5,266,684 - -0.1
5,267,023 - psuK JDDGAC_27410 0.31 +0.1
5,267,864 - psuG JDDGAC_27415 0.21 +0.3
5,268,037 + psuG JDDGAC_27415 0.40 -0.3
5,268,043 - psuG JDDGAC_27415 0.40 -2.0
5,268,192 - psuG JDDGAC_27415 0.56 -1.7
5,268,403 + psuG JDDGAC_27415 0.79 -1.1
5,268,404 - psuG JDDGAC_27415 0.79 -2.6
5,268,643 + +1.8
5,268,643 + +0.3
5,268,702 + -0.6
5,268,702 + -0.0
5,268,703 - -0.2
5,268,742 - +1.5
5,268,861 + psuT JDDGAC_27420 0.13 +1.3
5,268,899 + psuT JDDGAC_27420 0.16 -0.4
5,268,900 - psuT JDDGAC_27420 0.16 -1.5
5,269,043 + psuT JDDGAC_27420 0.28 -2.2
5,269,043 + psuT JDDGAC_27420 0.28 -0.6
5,269,043 + psuT JDDGAC_27420 0.28 -0.9
5,269,222 + psuT JDDGAC_27420 0.42 -0.2
5,269,348 + psuT JDDGAC_27420 0.52 +1.3
5,269,348 + psuT JDDGAC_27420 0.52 +0.0
5,269,349 - psuT JDDGAC_27420 0.52 +2.4
5,269,511 - psuT JDDGAC_27420 0.65 +2.2

Or see this region's nucleotide sequence