Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menD and menH overlap by 4 nucleotides menH and menB are separated by 14 nucleotides menB and menC overlap by 1 nucleotides
JDDGAC_26900: menD - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene- 1-carboxylic-acid synthase, at 5,152,498 to 5,154,168
menD
JDDGAC_26905: menH - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase, at 5,154,165 to 5,154,923
menH
JDDGAC_26910: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 5,154,938 to 5,155,795
menB
JDDGAC_26915: menC - o-succinylbenzoate synthase, at 5,155,795 to 5,156,757
menC
Position (kb) 5154
5155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5153.413 kb on + strand, within menD at 5153.474 kb on + strand, within menD at 5153.474 kb on + strand, within menD at 5153.484 kb on + strand, within menD at 5153.632 kb on + strand, within menD at 5153.726 kb on - strand, within menD at 5153.837 kb on - strand, within menD at 5153.837 kb on - strand, within menD at 5153.885 kb on + strand, within menD at 5153.888 kb on + strand, within menD at 5153.888 kb on + strand, within menD at 5153.889 kb on - strand, within menD at 5153.889 kb on - strand, within menD at 5153.889 kb on - strand, within menD at 5153.962 kb on + strand, within menD at 5154.020 kb on - strand at 5154.057 kb on - strand at 5154.165 kb on - strand at 5154.294 kb on + strand, within menH at 5154.294 kb on + strand, within menH at 5154.295 kb on - strand, within menH at 5154.371 kb on + strand, within menH at 5154.778 kb on + strand, within menH at 5154.778 kb on + strand, within menH at 5154.779 kb on - strand, within menH at 5154.889 kb on + strand at 5154.890 kb on - strand at 5154.965 kb on + strand at 5155.054 kb on + strand, within menB at 5155.096 kb on - strand, within menB at 5155.228 kb on + strand, within menB at 5155.381 kb on + strand, within menB at 5155.382 kb on - strand, within menB at 5155.709 kb on + strand, within menB at 5155.710 kb on - strand at 5155.811 kb on + strand at 5155.811 kb on + strand at 5155.812 kb on - strand at 5155.813 kb on + strand at 5155.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 5,153,413 + menD JDDGAC_26900 0.55 -1.7 5,153,474 + menD JDDGAC_26900 0.58 -1.1 5,153,474 + menD JDDGAC_26900 0.58 +2.0 5,153,484 + menD JDDGAC_26900 0.59 +0.3 5,153,632 + menD JDDGAC_26900 0.68 +0.2 5,153,726 - menD JDDGAC_26900 0.73 -1.0 5,153,837 - menD JDDGAC_26900 0.80 +0.7 5,153,837 - menD JDDGAC_26900 0.80 -1.3 5,153,885 + menD JDDGAC_26900 0.83 +0.4 5,153,888 + menD JDDGAC_26900 0.83 -0.2 5,153,888 + menD JDDGAC_26900 0.83 -0.7 5,153,889 - menD JDDGAC_26900 0.83 -0.1 5,153,889 - menD JDDGAC_26900 0.83 -0.8 5,153,889 - menD JDDGAC_26900 0.83 +0.8 5,153,962 + menD JDDGAC_26900 0.88 +0.1 5,154,020 - +1.8 5,154,057 - -1.8 5,154,165 - -3.1 5,154,294 + menH JDDGAC_26905 0.17 -1.7 5,154,294 + menH JDDGAC_26905 0.17 -1.0 5,154,295 - menH JDDGAC_26905 0.17 +0.1 5,154,371 + menH JDDGAC_26905 0.27 -0.6 5,154,778 + menH JDDGAC_26905 0.81 -1.8 5,154,778 + menH JDDGAC_26905 0.81 -0.6 5,154,779 - menH JDDGAC_26905 0.81 -1.1 5,154,889 + +0.7 5,154,890 - +0.3 5,154,965 + -0.1 5,155,054 + menB JDDGAC_26910 0.14 +0.3 5,155,096 - menB JDDGAC_26910 0.18 +0.7 5,155,228 + menB JDDGAC_26910 0.34 -1.1 5,155,381 + menB JDDGAC_26910 0.52 -2.3 5,155,382 - menB JDDGAC_26910 0.52 +0.0 5,155,709 + menB JDDGAC_26910 0.90 +1.4 5,155,710 - +2.0 5,155,811 + +0.1 5,155,811 + -1.2 5,155,812 - -0.3 5,155,813 + -0.7 5,155,814 - -0.7
Or see this region's nucleotide sequence