Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt actS and Gly_trna are separated by 78 nucleotides Gly_trna and JDDGAC_23370 are separated by 233 nucleotides JDDGAC_23370 and JDDGAC_23375 overlap by 17 nucleotides
JDDGAC_23360: actS - amidase activator ActS, at 4,479,770 to 4,480,525
actS
JDDGAC_23365: Gly_trna - tRNA-Gly(ccc), at 4,480,604 to 4,480,677
_trna
JDDGAC_23370: JDDGAC_23370 - Glyco-hydro-15 domain-containing protein, at 4,480,911 to 4,481,474
_23370
JDDGAC_23375: JDDGAC_23375 - Glyco-hydro-15 domain-containing protein, at 4,481,458 to 4,482,516
_23375
Position (kb) 4480
4481 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4479.622 kb on - strand at 4479.631 kb on - strand at 4479.644 kb on + strand at 4479.667 kb on + strand at 4479.668 kb on - strand at 4479.680 kb on + strand at 4479.684 kb on - strand at 4479.684 kb on - strand at 4479.733 kb on - strand at 4479.733 kb on - strand at 4479.738 kb on - strand at 4479.878 kb on + strand, within actS at 4479.879 kb on - strand, within actS at 4479.894 kb on - strand, within actS at 4479.918 kb on + strand, within actS at 4479.918 kb on + strand, within actS at 4479.918 kb on + strand, within actS at 4479.918 kb on + strand, within actS at 4479.918 kb on + strand, within actS at 4479.920 kb on + strand, within actS at 4479.921 kb on - strand, within actS at 4479.925 kb on + strand, within actS at 4479.926 kb on - strand, within actS at 4479.951 kb on + strand, within actS at 4479.952 kb on - strand, within actS at 4479.990 kb on - strand, within actS at 4480.036 kb on + strand, within actS at 4480.037 kb on - strand, within actS at 4480.042 kb on + strand, within actS at 4480.042 kb on + strand, within actS at 4480.042 kb on + strand, within actS at 4480.043 kb on - strand, within actS at 4480.046 kb on - strand, within actS at 4480.046 kb on - strand, within actS at 4480.051 kb on + strand, within actS at 4480.091 kb on - strand, within actS at 4480.217 kb on - strand, within actS at 4480.240 kb on + strand, within actS at 4480.241 kb on - strand, within actS at 4480.241 kb on - strand, within actS at 4480.271 kb on + strand, within actS at 4480.271 kb on + strand, within actS at 4480.271 kb on + strand, within actS at 4480.272 kb on - strand, within actS at 4480.272 kb on - strand, within actS at 4480.332 kb on - strand, within actS at 4480.369 kb on - strand, within actS at 4480.373 kb on - strand, within actS at 4480.491 kb on - strand at 4480.491 kb on - strand at 4480.581 kb on + strand at 4480.584 kb on - strand at 4480.622 kb on + strand, within Gly_trna at 4480.648 kb on - strand, within Gly_trna at 4480.726 kb on + strand at 4480.826 kb on + strand at 4480.843 kb on + strand at 4480.879 kb on - strand at 4481.050 kb on - strand, within JDDGAC_23370 at 4481.083 kb on - strand, within JDDGAC_23370 at 4481.083 kb on - strand, within JDDGAC_23370 at 4481.292 kb on - strand, within JDDGAC_23370 at 4481.305 kb on - strand, within JDDGAC_23370 at 4481.305 kb on - strand, within JDDGAC_23370 at 4481.307 kb on - strand, within JDDGAC_23370 at 4481.375 kb on + strand, within JDDGAC_23370 at 4481.375 kb on + strand, within JDDGAC_23370 at 4481.376 kb on - strand, within JDDGAC_23370 at 4481.376 kb on - strand, within JDDGAC_23370 at 4481.418 kb on - strand at 4481.536 kb on - strand at 4481.576 kb on + strand, within JDDGAC_23375 at 4481.576 kb on + strand, within JDDGAC_23375 at 4481.577 kb on - strand, within JDDGAC_23375 at 4481.577 kb on - strand, within JDDGAC_23375 at 4481.577 kb on - strand, within JDDGAC_23375 at 4481.596 kb on - strand, within JDDGAC_23375 at 4481.598 kb on + strand, within JDDGAC_23375 at 4481.599 kb on - strand, within JDDGAC_23375 at 4481.599 kb on - strand, within JDDGAC_23375 at 4481.668 kb on - strand, within JDDGAC_23375
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 4,479,622 - -0.4 4,479,631 - +0.4 4,479,644 + +1.5 4,479,667 + +0.3 4,479,668 - +1.0 4,479,680 + -0.5 4,479,684 - -0.4 4,479,684 - -1.0 4,479,733 - -0.4 4,479,733 - +1.0 4,479,738 - -0.6 4,479,878 + actS JDDGAC_23360 0.14 -2.7 4,479,879 - actS JDDGAC_23360 0.14 +1.1 4,479,894 - actS JDDGAC_23360 0.16 +1.0 4,479,918 + actS JDDGAC_23360 0.20 -2.2 4,479,918 + actS JDDGAC_23360 0.20 -0.9 4,479,918 + actS JDDGAC_23360 0.20 +1.5 4,479,918 + actS JDDGAC_23360 0.20 -0.2 4,479,918 + actS JDDGAC_23360 0.20 +0.5 4,479,920 + actS JDDGAC_23360 0.20 +1.8 4,479,921 - actS JDDGAC_23360 0.20 -1.4 4,479,925 + actS JDDGAC_23360 0.21 -0.2 4,479,926 - actS JDDGAC_23360 0.21 -0.5 4,479,951 + actS JDDGAC_23360 0.24 +0.8 4,479,952 - actS JDDGAC_23360 0.24 +0.2 4,479,990 - actS JDDGAC_23360 0.29 -1.2 4,480,036 + actS JDDGAC_23360 0.35 +0.9 4,480,037 - actS JDDGAC_23360 0.35 +0.3 4,480,042 + actS JDDGAC_23360 0.36 +0.6 4,480,042 + actS JDDGAC_23360 0.36 -0.1 4,480,042 + actS JDDGAC_23360 0.36 -0.2 4,480,043 - actS JDDGAC_23360 0.36 -0.9 4,480,046 - actS JDDGAC_23360 0.37 +1.3 4,480,046 - actS JDDGAC_23360 0.37 +0.4 4,480,051 + actS JDDGAC_23360 0.37 +0.5 4,480,091 - actS JDDGAC_23360 0.42 +1.4 4,480,217 - actS JDDGAC_23360 0.59 +0.2 4,480,240 + actS JDDGAC_23360 0.62 -0.2 4,480,241 - actS JDDGAC_23360 0.62 -1.0 4,480,241 - actS JDDGAC_23360 0.62 -1.4 4,480,271 + actS JDDGAC_23360 0.66 -0.6 4,480,271 + actS JDDGAC_23360 0.66 +1.5 4,480,271 + actS JDDGAC_23360 0.66 +0.3 4,480,272 - actS JDDGAC_23360 0.66 +0.5 4,480,272 - actS JDDGAC_23360 0.66 -0.5 4,480,332 - actS JDDGAC_23360 0.74 +0.7 4,480,369 - actS JDDGAC_23360 0.79 +1.5 4,480,373 - actS JDDGAC_23360 0.80 +0.5 4,480,491 - +0.3 4,480,491 - +0.6 4,480,581 + +0.5 4,480,584 - -0.6 4,480,622 + Gly_trna JDDGAC_23365 0.24 -1.4 4,480,648 - Gly_trna JDDGAC_23365 0.59 -2.8 4,480,726 + -1.1 4,480,826 + -1.5 4,480,843 + +0.5 4,480,879 - +0.9 4,481,050 - JDDGAC_23370 0.25 +1.3 4,481,083 - JDDGAC_23370 0.30 +0.9 4,481,083 - JDDGAC_23370 0.30 +1.8 4,481,292 - JDDGAC_23370 0.68 +0.0 4,481,305 - JDDGAC_23370 0.70 -0.0 4,481,305 - JDDGAC_23370 0.70 +0.7 4,481,307 - JDDGAC_23370 0.70 +0.2 4,481,375 + JDDGAC_23370 0.82 -0.5 4,481,375 + JDDGAC_23370 0.82 +0.8 4,481,376 - JDDGAC_23370 0.82 +0.5 4,481,376 - JDDGAC_23370 0.82 -0.8 4,481,418 - -0.4 4,481,536 - -0.3 4,481,576 + JDDGAC_23375 0.11 -1.1 4,481,576 + JDDGAC_23375 0.11 -0.9 4,481,577 - JDDGAC_23375 0.11 +1.7 4,481,577 - JDDGAC_23375 0.11 +0.8 4,481,577 - JDDGAC_23375 0.11 +0.7 4,481,596 - JDDGAC_23375 0.13 -1.2 4,481,598 + JDDGAC_23375 0.13 +0.3 4,481,599 - JDDGAC_23375 0.13 -1.8 4,481,599 - JDDGAC_23375 0.13 +1.7 4,481,668 - JDDGAC_23375 0.20 -1.7
Or see this region's nucleotide sequence