Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiT and yhbS overlap by 7 nucleotides yhbS and yhbQ overlap by 14 nucleotides yhbQ and yhbP are separated by 50 nucleotides yhbP and yhbO overlap by 21 nucleotides yhbO and yraR are separated by 127 nucleotides
JDDGAC_21805: ubiT - Ubiquinone biosynthesis accessory factor UbiT, at 4,174,309 to 4,174,833
ubiT
JDDGAC_21810: yhbS - Uncharacterized N-acetyltransferase YhbS, at 4,174,827 to 4,175,330
yhbS
JDDGAC_21815: yhbQ - DNA damage response exodeoxyribonuclease YhbQ, at 4,175,317 to 4,175,619
yhbQ
JDDGAC_21820: yhbP - YhbP family protein, at 4,175,670 to 4,176,113
yhbP
JDDGAC_21825: yhbO - protein/nucleic acid deglycase, at 4,176,093 to 4,176,611
yhbO
JDDGAC_21830: yraR - Putative NAD(P)-binding protein YraR, at 4,176,739 to 4,177,374
yraR
Position (kb) 4175
4176
4177 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4174.688 kb on - strand, within ubiT at 4174.688 kb on - strand, within ubiT at 4174.740 kb on - strand, within ubiT at 4174.992 kb on + strand, within yhbS at 4174.993 kb on - strand, within yhbS at 4174.993 kb on - strand, within yhbS at 4174.993 kb on - strand, within yhbS at 4175.003 kb on + strand, within yhbS at 4175.173 kb on + strand, within yhbS at 4175.254 kb on + strand, within yhbS at 4175.287 kb on + strand at 4175.288 kb on - strand at 4175.304 kb on + strand at 4175.354 kb on - strand, within yhbQ at 4175.569 kb on - strand, within yhbQ at 4175.570 kb on + strand, within yhbQ at 4175.570 kb on + strand, within yhbQ at 4175.570 kb on + strand, within yhbQ at 4175.571 kb on - strand, within yhbQ at 4175.578 kb on - strand, within yhbQ at 4175.654 kb on - strand at 4175.655 kb on + strand at 4175.656 kb on - strand at 4175.656 kb on - strand at 4175.890 kb on + strand, within yhbP at 4175.916 kb on - strand, within yhbP at 4175.970 kb on + strand, within yhbP at 4175.970 kb on + strand, within yhbP at 4176.086 kb on - strand at 4176.112 kb on - strand at 4176.345 kb on - strand, within yhbO at 4176.376 kb on + strand, within yhbO at 4176.446 kb on + strand, within yhbO at 4176.447 kb on - strand, within yhbO at 4176.512 kb on + strand, within yhbO at 4176.558 kb on - strand, within yhbO at 4176.667 kb on + strand at 4176.667 kb on + strand at 4176.668 kb on - strand at 4176.668 kb on - strand at 4176.668 kb on - strand at 4176.679 kb on + strand at 4176.679 kb on + strand at 4176.679 kb on + strand at 4176.680 kb on - strand at 4176.680 kb on - strand at 4176.682 kb on + strand at 4176.683 kb on - strand at 4176.726 kb on + strand at 4176.859 kb on - strand, within yraR at 4176.869 kb on + strand, within yraR at 4176.869 kb on + strand, within yraR at 4176.873 kb on + strand, within yraR at 4176.873 kb on + strand, within yraR at 4176.873 kb on + strand, within yraR at 4176.987 kb on + strand, within yraR at 4177.053 kb on + strand, within yraR at 4177.071 kb on + strand, within yraR at 4177.102 kb on + strand, within yraR
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 4,174,688 - ubiT JDDGAC_21805 0.72 -0.3 4,174,688 - ubiT JDDGAC_21805 0.72 -0.8 4,174,740 - ubiT JDDGAC_21805 0.82 -0.1 4,174,992 + yhbS JDDGAC_21810 0.33 +0.3 4,174,993 - yhbS JDDGAC_21810 0.33 +1.2 4,174,993 - yhbS JDDGAC_21810 0.33 +0.2 4,174,993 - yhbS JDDGAC_21810 0.33 -0.6 4,175,003 + yhbS JDDGAC_21810 0.35 -0.3 4,175,173 + yhbS JDDGAC_21810 0.69 -1.3 4,175,254 + yhbS JDDGAC_21810 0.85 -0.3 4,175,287 + -1.1 4,175,288 - -1.5 4,175,304 + +1.0 4,175,354 - yhbQ JDDGAC_21815 0.12 +0.5 4,175,569 - yhbQ JDDGAC_21815 0.83 +0.1 4,175,570 + yhbQ JDDGAC_21815 0.83 -0.0 4,175,570 + yhbQ JDDGAC_21815 0.83 -1.2 4,175,570 + yhbQ JDDGAC_21815 0.83 +0.3 4,175,571 - yhbQ JDDGAC_21815 0.84 -0.7 4,175,578 - yhbQ JDDGAC_21815 0.86 +0.3 4,175,654 - +0.7 4,175,655 + +0.1 4,175,656 - -0.1 4,175,656 - -0.8 4,175,890 + yhbP JDDGAC_21820 0.50 +1.3 4,175,916 - yhbP JDDGAC_21820 0.55 -0.9 4,175,970 + yhbP JDDGAC_21820 0.68 -1.1 4,175,970 + yhbP JDDGAC_21820 0.68 -1.2 4,176,086 - -0.3 4,176,112 - +1.9 4,176,345 - yhbO JDDGAC_21825 0.49 -0.2 4,176,376 + yhbO JDDGAC_21825 0.55 +0.1 4,176,446 + yhbO JDDGAC_21825 0.68 -0.7 4,176,447 - yhbO JDDGAC_21825 0.68 +1.1 4,176,512 + yhbO JDDGAC_21825 0.81 -1.6 4,176,558 - yhbO JDDGAC_21825 0.90 +0.3 4,176,667 + +0.1 4,176,667 + -0.0 4,176,668 - -0.8 4,176,668 - -0.4 4,176,668 - -0.5 4,176,679 + +0.8 4,176,679 + +0.5 4,176,679 + -0.3 4,176,680 - +0.0 4,176,680 - -1.4 4,176,682 + -0.1 4,176,683 - -1.8 4,176,726 + -0.3 4,176,859 - yraR JDDGAC_21830 0.19 +2.0 4,176,869 + yraR JDDGAC_21830 0.20 -2.4 4,176,869 + yraR JDDGAC_21830 0.20 +0.3 4,176,873 + yraR JDDGAC_21830 0.21 +1.8 4,176,873 + yraR JDDGAC_21830 0.21 +1.3 4,176,873 + yraR JDDGAC_21830 0.21 -0.2 4,176,987 + yraR JDDGAC_21830 0.39 +0.2 4,177,053 + yraR JDDGAC_21830 0.49 +0.6 4,177,071 + yraR JDDGAC_21830 0.52 +0.2 4,177,102 + yraR JDDGAC_21830 0.57 +0.4
Or see this region's nucleotide sequence