Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt int and icd are separated by 113 nucleotides icd and rluE are separated by 171 nucleotides rluE and nudJ are separated by 9 nucleotides
JDDGAC_06600: int - Integrase, at 1,149,810 to 1,150,952
int
JDDGAC_06605: icd - NADP-dependent isocitrate dehydrogenase, at 1,151,066 to 1,152,316
icd
JDDGAC_06610: rluE - 23S rRNA pseudouridine(2457) synthase RluE, at 1,152,488 to 1,153,141
rluE
JDDGAC_06615: nudJ - phosphatase NudJ, at 1,153,151 to 1,153,612
nudJ
Position (kb) 1151
1152
1153 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1150.082 kb on + strand, within int at 1150.127 kb on - strand, within int at 1150.432 kb on + strand, within int at 1150.550 kb on + strand, within int at 1150.550 kb on + strand, within int at 1150.550 kb on + strand, within int at 1150.616 kb on + strand, within int at 1150.631 kb on + strand, within int at 1150.831 kb on + strand, within int at 1150.831 kb on + strand, within int at 1150.831 kb on + strand, within int at 1150.874 kb on + strand at 1150.874 kb on + strand at 1151.015 kb on - strand at 1151.146 kb on - strand at 1151.252 kb on - strand, within icd at 1151.486 kb on - strand, within icd at 1151.723 kb on - strand, within icd at 1152.368 kb on - strand at 1152.484 kb on + strand at 1152.536 kb on - strand at 1152.575 kb on - strand, within rluE at 1152.583 kb on + strand, within rluE at 1152.704 kb on + strand, within rluE at 1152.704 kb on + strand, within rluE at 1152.812 kb on + strand, within rluE at 1152.812 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.880 kb on - strand, within rluE at 1153.002 kb on + strand, within rluE at 1153.002 kb on + strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.092 kb on - strand at 1153.139 kb on + strand at 1153.139 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 1,150,082 + int JDDGAC_06600 0.24 +0.4 1,150,127 - int JDDGAC_06600 0.28 +0.2 1,150,432 + int JDDGAC_06600 0.54 +1.8 1,150,550 + int JDDGAC_06600 0.65 +1.9 1,150,550 + int JDDGAC_06600 0.65 -0.6 1,150,550 + int JDDGAC_06600 0.65 +1.3 1,150,616 + int JDDGAC_06600 0.71 +1.3 1,150,631 + int JDDGAC_06600 0.72 +0.5 1,150,831 + int JDDGAC_06600 0.89 -1.3 1,150,831 + int JDDGAC_06600 0.89 +0.1 1,150,831 + int JDDGAC_06600 0.89 -1.3 1,150,874 + +0.6 1,150,874 + -2.5 1,151,015 - -0.5 1,151,146 - +0.0 1,151,252 - icd JDDGAC_06605 0.15 -0.6 1,151,486 - icd JDDGAC_06605 0.34 -1.6 1,151,723 - icd JDDGAC_06605 0.53 -1.3 1,152,368 - -2.4 1,152,484 + +0.1 1,152,536 - +0.5 1,152,575 - rluE JDDGAC_06610 0.13 -1.3 1,152,583 + rluE JDDGAC_06610 0.15 -0.6 1,152,704 + rluE JDDGAC_06610 0.33 -0.9 1,152,704 + rluE JDDGAC_06610 0.33 +0.6 1,152,812 + rluE JDDGAC_06610 0.50 +0.0 1,152,812 + rluE JDDGAC_06610 0.50 +0.4 1,152,815 + rluE JDDGAC_06610 0.50 -1.4 1,152,815 + rluE JDDGAC_06610 0.50 +2.0 1,152,815 + rluE JDDGAC_06610 0.50 -1.3 1,152,880 - rluE JDDGAC_06610 0.60 +0.7 1,153,002 + rluE JDDGAC_06610 0.79 -0.3 1,153,002 + rluE JDDGAC_06610 0.79 +1.4 1,153,003 - rluE JDDGAC_06610 0.79 -1.7 1,153,003 - rluE JDDGAC_06610 0.79 -0.2 1,153,003 - rluE JDDGAC_06610 0.79 -0.7 1,153,004 + rluE JDDGAC_06610 0.79 +0.3 1,153,004 + rluE JDDGAC_06610 0.79 +1.5 1,153,004 + rluE JDDGAC_06610 0.79 +1.6 1,153,004 + rluE JDDGAC_06610 0.79 -0.4 1,153,092 - -1.7 1,153,139 + +1.7 1,153,139 + -0.6
Or see this region's nucleotide sequence