Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fnr and uspE are separated by 151 nucleotides uspE and ynaJ are separated by 49 nucleotides ynaJ and ynaI are separated by 243 nucleotides
JDDGAC_04785: fnr - fumarate/nitrate reduction transcriptional regulator Fnr, at 830,236 to 830,988
fnr
JDDGAC_04790: uspE - universal stress protein UspE, at 831,140 to 832,090
uspE
JDDGAC_04795: ynaJ - Uncharacterized protein YnaJ, at 832,140 to 832,397
ynaJ
JDDGAC_04800: ynaI - low conductance mechanosensitive channel YnaI, at 832,641 to 833,672
ynaI
Position (kb) 831
832
833 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 830.177 kb on - strand at 830.235 kb on + strand at 830.256 kb on + strand at 830.256 kb on + strand at 830.257 kb on - strand at 830.364 kb on + strand, within fnr at 830.472 kb on + strand, within fnr at 830.597 kb on + strand, within fnr at 830.923 kb on + strand at 830.923 kb on + strand at 831.000 kb on - strand at 831.020 kb on + strand at 831.126 kb on + strand at 831.126 kb on + strand at 831.127 kb on - strand at 831.219 kb on + strand at 831.263 kb on + strand, within uspE at 831.275 kb on + strand, within uspE at 831.275 kb on + strand, within uspE at 831.748 kb on + strand, within uspE at 831.921 kb on + strand, within uspE at 832.138 kb on - strand at 832.196 kb on + strand, within ynaJ at 832.209 kb on - strand, within ynaJ at 832.285 kb on - strand, within ynaJ at 832.402 kb on + strand at 832.403 kb on - strand at 832.518 kb on - strand at 832.550 kb on + strand at 832.551 kb on - strand at 832.762 kb on + strand, within ynaI at 832.795 kb on + strand, within ynaI at 832.887 kb on - strand, within ynaI
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 830,177 - -0.3 830,235 + +2.5 830,256 + +1.0 830,256 + -1.5 830,257 - -1.8 830,364 + fnr JDDGAC_04785 0.17 -1.3 830,472 + fnr JDDGAC_04785 0.31 +1.1 830,597 + fnr JDDGAC_04785 0.48 -2.8 830,923 + +2.2 830,923 + +0.1 831,000 - -1.5 831,020 + -0.4 831,126 + -1.5 831,126 + +0.6 831,127 - -0.4 831,219 + -0.5 831,263 + uspE JDDGAC_04790 0.13 -1.1 831,275 + uspE JDDGAC_04790 0.14 -0.1 831,275 + uspE JDDGAC_04790 0.14 +0.4 831,748 + uspE JDDGAC_04790 0.64 -3.9 831,921 + uspE JDDGAC_04790 0.82 -1.7 832,138 - -0.4 832,196 + ynaJ JDDGAC_04795 0.22 -1.3 832,209 - ynaJ JDDGAC_04795 0.27 -0.0 832,285 - ynaJ JDDGAC_04795 0.56 -0.4 832,402 + +1.1 832,403 - -1.1 832,518 - -0.7 832,550 + +3.4 832,551 - -1.0 832,762 + ynaI JDDGAC_04800 0.12 +0.6 832,795 + ynaI JDDGAC_04800 0.15 -0.4 832,887 - ynaI JDDGAC_04800 0.24 +2.0
Or see this region's nucleotide sequence