Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaC and JDDGAC_02480 are separated by 126 nucleotides JDDGAC_02480 and JDDGAC_02485 are separated by 15 nucleotides JDDGAC_02485 and JDDGAC_02490 are separated by 56 nucleotides JDDGAC_02490 and JDDGAC_02495 are separated by 115 nucleotides JDDGAC_02495 and JDDGAC_02500 overlap by 12 nucleotides
JDDGAC_02475: dnaC - DNA replication protein DnaC, at 443,845 to 444,585
dnaC
JDDGAC_02480: JDDGAC_02480 - DUF1627 domain-containing protein, at 444,712 to 445,380
_02480
JDDGAC_02485: JDDGAC_02485 - DUF977 domain-containing protein, at 445,396 to 445,791
_02485
JDDGAC_02490: JDDGAC_02490 - DUF551 domain-containing protein, at 445,848 to 446,432
_02490
JDDGAC_02495: JDDGAC_02495 - hypothetical protein, at 446,548 to 446,652
_02495
JDDGAC_02500: JDDGAC_02500 - hypothetical protein, at 446,641 to 446,796
_02500
Position (kb)
445
446 Strain fitness (log2 ratio)
-2
-1
0
1 at 444.507 kb on + strand, within dnaC at 444.579 kb on + strand at 444.580 kb on - strand at 444.703 kb on + strand at 444.807 kb on - strand, within JDDGAC_02480 at 445.035 kb on + strand, within JDDGAC_02480 at 445.564 kb on + strand, within JDDGAC_02485 at 445.564 kb on + strand, within JDDGAC_02485 at 445.575 kb on + strand, within JDDGAC_02485 at 445.760 kb on - strand at 445.897 kb on + strand at 445.898 kb on - strand at 445.966 kb on + strand, within JDDGAC_02490 at 446.016 kb on + strand, within JDDGAC_02490 at 446.018 kb on + strand, within JDDGAC_02490 at 446.088 kb on + strand, within JDDGAC_02490 at 446.088 kb on + strand, within JDDGAC_02490 at 446.088 kb on + strand, within JDDGAC_02490 at 446.098 kb on + strand, within JDDGAC_02490 at 446.099 kb on - strand, within JDDGAC_02490 at 446.099 kb on - strand, within JDDGAC_02490 at 446.101 kb on - strand, within JDDGAC_02490
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 444,507 + dnaC JDDGAC_02475 0.89 +1.7 444,579 + -2.5 444,580 - -0.7 444,703 + +0.1 444,807 - JDDGAC_02480 0.14 +0.2 445,035 + JDDGAC_02480 0.48 +0.7 445,564 + JDDGAC_02485 0.42 -1.7 445,564 + JDDGAC_02485 0.42 -2.1 445,575 + JDDGAC_02485 0.45 -1.3 445,760 - +1.1 445,897 + -0.7 445,898 - -0.4 445,966 + JDDGAC_02490 0.20 -1.6 446,016 + JDDGAC_02490 0.29 -1.4 446,018 + JDDGAC_02490 0.29 -0.9 446,088 + JDDGAC_02490 0.41 -0.4 446,088 + JDDGAC_02490 0.41 -0.7 446,088 + JDDGAC_02490 0.41 +1.5 446,098 + JDDGAC_02490 0.43 +0.2 446,099 - JDDGAC_02490 0.43 +1.6 446,099 - JDDGAC_02490 0.43 +0.7 446,101 - JDDGAC_02490 0.43 -2.6
Or see this region's nucleotide sequence