Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdtJ and mdtI overlap by 14 nucleotides mdtI and ydgD are separated by 38 nucleotides ydgD and ydgU are separated by 99 nucleotides ydgU and asr are separated by 92 nucleotides
JDDGAC_02295: mdtJ - multidrug/spermidine efflux SMR transporter subunit MdtJ, at 417,251 to 417,616
mdtJ
JDDGAC_02300: mdtI - multidrug/spermidine efflux SMR transporter subunit MdtI, at 417,603 to 417,932
mdtI
JDDGAC_02305: ydgD - Uncharacterized serine protease YdgD, at 417,971 to 418,792
ydgD
JDDGAC_02310: ydgU - Uncharacterized protein YdgU, at 418,892 to 418,975
ydgU
JDDGAC_02315: asr - acid resistance repetitive basic protein Asr, at 419,068 to 419,376
asr
Position (kb) 417
418
419 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 416.996 kb on + strand at 417.102 kb on + strand at 417.795 kb on + strand, within mdtI at 417.872 kb on + strand, within mdtI at 417.973 kb on - strand at 418.102 kb on + strand, within ydgD at 418.206 kb on + strand, within ydgD at 418.253 kb on - strand, within ydgD at 418.292 kb on - strand, within ydgD at 418.292 kb on - strand, within ydgD at 418.337 kb on - strand, within ydgD at 418.383 kb on - strand, within ydgD at 418.446 kb on - strand, within ydgD at 418.446 kb on - strand, within ydgD at 418.585 kb on + strand, within ydgD at 418.786 kb on + strand at 418.787 kb on - strand at 418.787 kb on - strand at 418.787 kb on - strand at 418.792 kb on + strand at 418.813 kb on + strand at 418.842 kb on + strand at 418.843 kb on - strand at 418.887 kb on + strand at 418.887 kb on + strand at 418.942 kb on + strand, within ydgU at 418.943 kb on - strand, within ydgU at 418.975 kb on + strand at 418.975 kb on + strand at 418.978 kb on + strand at 418.988 kb on + strand at 419.023 kb on + strand at 419.055 kb on + strand at 419.069 kb on + strand at 419.184 kb on + strand, within asr at 419.185 kb on - strand, within asr at 419.434 kb on + strand at 419.488 kb on + strand at 419.629 kb on + strand at 419.666 kb on + strand at 419.670 kb on + strand at 419.670 kb on + strand at 419.671 kb on - strand at 419.735 kb on + strand at 419.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 416,996 + -1.4 417,102 + +0.5 417,795 + mdtI JDDGAC_02300 0.58 +0.9 417,872 + mdtI JDDGAC_02300 0.82 -1.0 417,973 - -0.6 418,102 + ydgD JDDGAC_02305 0.16 +0.2 418,206 + ydgD JDDGAC_02305 0.29 -2.1 418,253 - ydgD JDDGAC_02305 0.34 +0.2 418,292 - ydgD JDDGAC_02305 0.39 -2.5 418,292 - ydgD JDDGAC_02305 0.39 -0.7 418,337 - ydgD JDDGAC_02305 0.45 -0.3 418,383 - ydgD JDDGAC_02305 0.50 -1.9 418,446 - ydgD JDDGAC_02305 0.58 -0.4 418,446 - ydgD JDDGAC_02305 0.58 -4.5 418,585 + ydgD JDDGAC_02305 0.75 -0.6 418,786 + +1.1 418,787 - +0.4 418,787 - -0.2 418,787 - -2.6 418,792 + +0.8 418,813 + -0.1 418,842 + +1.1 418,843 - +0.1 418,887 + -0.6 418,887 + -1.5 418,942 + ydgU JDDGAC_02310 0.60 +0.0 418,943 - ydgU JDDGAC_02310 0.61 +0.3 418,975 + +0.6 418,975 + -0.5 418,978 + +2.0 418,988 + -0.6 419,023 + -0.8 419,055 + -2.0 419,069 + +1.9 419,184 + asr JDDGAC_02315 0.38 +1.0 419,185 - asr JDDGAC_02315 0.38 +2.4 419,434 + +0.5 419,488 + -0.8 419,629 + +1.9 419,666 + -0.7 419,670 + -0.3 419,670 + +1.1 419,671 - -0.9 419,735 + +0.4 419,760 + -0.0
Or see this region's nucleotide sequence