Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsxC and rsxB overlap by 8 nucleotides rsxB and rsxA overlap by 1 nucleotides rsxA and ydgK are separated by 76 nucleotides ydgK and ydgT are separated by 85 nucleotides
JDDGAC_02135: rsxC - electron transport complex subunit RsxC, at 381,685 to 384,003
rsxC
JDDGAC_02140: rsxB - electron transport complex subunit RsxB, at 383,996 to 384,574
rsxB
JDDGAC_02145: rsxA - electron transport complex subunit RsxA, at 384,574 to 385,155
rsxA
JDDGAC_02150: ydgK - Inner membrane protein YdgK, at 385,232 to 385,672
ydgK
JDDGAC_02155: ydgT - transcription modulator YdgT, at 385,758 to 385,973
ydgT
Position (kb) 384
385
386 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 383.599 kb on + strand, within rsxC at 383.676 kb on + strand, within rsxC at 383.810 kb on - strand at 383.810 kb on - strand at 383.998 kb on - strand at 384.119 kb on + strand, within rsxB at 384.120 kb on - strand, within rsxB at 384.212 kb on - strand, within rsxB at 384.576 kb on - strand at 384.619 kb on - strand at 384.858 kb on - strand, within rsxA at 384.955 kb on - strand, within rsxA at 385.121 kb on - strand at 385.183 kb on + strand at 385.400 kb on - strand, within ydgK at 385.405 kb on - strand, within ydgK at 385.462 kb on - strand, within ydgK at 385.462 kb on - strand, within ydgK at 385.464 kb on + strand, within ydgK at 385.573 kb on - strand, within ydgK at 385.595 kb on - strand, within ydgK at 385.598 kb on + strand, within ydgK at 385.672 kb on + strand at 385.673 kb on - strand at 385.673 kb on - strand at 385.673 kb on - strand at 385.673 kb on - strand at 385.673 kb on - strand at 385.715 kb on - strand at 385.729 kb on - strand at 385.729 kb on - strand at 385.769 kb on - strand at 385.856 kb on - strand, within ydgT at 385.859 kb on + strand, within ydgT at 385.860 kb on - strand, within ydgT at 385.860 kb on - strand, within ydgT at 385.974 kb on - strand at 385.985 kb on - strand at 386.057 kb on - strand at 386.110 kb on - strand at 386.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 383,599 + rsxC JDDGAC_02135 0.83 -0.8 383,676 + rsxC JDDGAC_02135 0.86 -0.0 383,810 - +0.2 383,810 - +0.5 383,998 - -1.5 384,119 + rsxB JDDGAC_02140 0.21 -0.4 384,120 - rsxB JDDGAC_02140 0.21 +1.3 384,212 - rsxB JDDGAC_02140 0.37 -3.5 384,576 - +0.3 384,619 - -1.4 384,858 - rsxA JDDGAC_02145 0.49 -0.8 384,955 - rsxA JDDGAC_02145 0.65 -2.6 385,121 - +0.2 385,183 + -1.6 385,400 - ydgK JDDGAC_02150 0.38 -1.8 385,405 - ydgK JDDGAC_02150 0.39 -1.6 385,462 - ydgK JDDGAC_02150 0.52 -1.0 385,462 - ydgK JDDGAC_02150 0.52 +0.4 385,464 + ydgK JDDGAC_02150 0.53 -0.1 385,573 - ydgK JDDGAC_02150 0.77 -1.7 385,595 - ydgK JDDGAC_02150 0.82 +1.3 385,598 + ydgK JDDGAC_02150 0.83 +0.7 385,672 + +2.6 385,673 - -0.2 385,673 - +0.3 385,673 - -0.0 385,673 - -0.8 385,673 - -1.6 385,715 - -0.4 385,729 - +0.0 385,729 - -0.9 385,769 - +0.9 385,856 - ydgT JDDGAC_02155 0.45 -2.3 385,859 + ydgT JDDGAC_02155 0.47 -1.4 385,860 - ydgT JDDGAC_02155 0.47 -1.8 385,860 - ydgT JDDGAC_02155 0.47 +0.7 385,974 - -2.5 385,985 - +2.0 386,057 - +0.1 386,110 - -0.3 386,134 - +0.0
Or see this region's nucleotide sequence