Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt exoX and yobB are separated by 23 nucleotides yobB and holE are separated by 101 nucleotides holE and yobA are separated by 138 nucleotides yobA and copD are separated by 3 nucleotides
JDDGAC_00960: exoX - exodeoxyribonuclease X, at 160,626 to 161,288
exoX
JDDGAC_00965: yobB - Uncharacterized protein YobB, at 161,312 to 161,968
yobB
JDDGAC_00970: holE - DNA polymerase III subunit theta, at 162,070 to 162,300
holE
JDDGAC_00975: yobA - CopC domain-containing protein YobA, at 162,439 to 162,813
yobA
JDDGAC_00980: copD - copper homeostasis membrane protein CopD, at 162,817 to 163,689
copD
Position (kb) 162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 161.077 kb on + strand, within exoX at 161.077 kb on + strand, within exoX at 161.160 kb on - strand, within exoX at 161.367 kb on - strand at 161.372 kb on + strand at 161.372 kb on + strand at 161.432 kb on - strand, within yobB at 161.563 kb on + strand, within yobB at 161.569 kb on + strand, within yobB at 161.569 kb on + strand, within yobB at 161.570 kb on - strand, within yobB at 161.570 kb on - strand, within yobB at 161.644 kb on - strand, within yobB at 161.704 kb on + strand, within yobB at 161.705 kb on - strand, within yobB at 161.705 kb on - strand, within yobB at 161.771 kb on + strand, within yobB at 161.771 kb on + strand, within yobB at 161.903 kb on + strand at 161.904 kb on - strand at 161.927 kb on - strand at 161.968 kb on + strand at 161.969 kb on - strand at 161.969 kb on - strand at 162.020 kb on - strand at 162.031 kb on - strand at 162.041 kb on - strand at 162.095 kb on - strand, within holE at 162.204 kb on + strand, within holE at 162.210 kb on - strand, within holE at 162.305 kb on - strand at 162.420 kb on - strand at 162.431 kb on + strand at 162.435 kb on + strand at 162.435 kb on + strand at 162.438 kb on + strand at 162.439 kb on - strand at 162.527 kb on + strand, within yobA at 162.739 kb on + strand, within yobA at 162.739 kb on + strand, within yobA at 162.739 kb on + strand, within yobA at 162.793 kb on + strand at 162.793 kb on + strand at 162.892 kb on + strand at 163.043 kb on + strand, within copD at 163.198 kb on - strand, within copD at 163.263 kb on + strand, within copD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 161,077 + exoX JDDGAC_00960 0.68 -1.1 161,077 + exoX JDDGAC_00960 0.68 +0.1 161,160 - exoX JDDGAC_00960 0.81 +0.3 161,367 - -2.4 161,372 + +1.3 161,372 + -2.5 161,432 - yobB JDDGAC_00965 0.18 -0.4 161,563 + yobB JDDGAC_00965 0.38 -0.6 161,569 + yobB JDDGAC_00965 0.39 -2.0 161,569 + yobB JDDGAC_00965 0.39 -3.3 161,570 - yobB JDDGAC_00965 0.39 +1.1 161,570 - yobB JDDGAC_00965 0.39 +1.6 161,644 - yobB JDDGAC_00965 0.51 -0.8 161,704 + yobB JDDGAC_00965 0.60 -0.7 161,705 - yobB JDDGAC_00965 0.60 +0.1 161,705 - yobB JDDGAC_00965 0.60 -0.3 161,771 + yobB JDDGAC_00965 0.70 +0.3 161,771 + yobB JDDGAC_00965 0.70 +0.9 161,903 + -3.6 161,904 - -2.4 161,927 - -2.1 161,968 + -2.0 161,969 - -2.0 161,969 - -2.0 162,020 - +0.8 162,031 - +0.7 162,041 - -2.2 162,095 - holE JDDGAC_00970 0.11 -0.6 162,204 + holE JDDGAC_00970 0.58 -0.9 162,210 - holE JDDGAC_00970 0.61 -0.9 162,305 - -1.8 162,420 - +0.4 162,431 + -0.7 162,435 + -0.5 162,435 + -2.3 162,438 + +1.0 162,439 - -2.1 162,527 + yobA JDDGAC_00975 0.23 -1.2 162,739 + yobA JDDGAC_00975 0.80 +2.0 162,739 + yobA JDDGAC_00975 0.80 +0.8 162,739 + yobA JDDGAC_00975 0.80 +0.1 162,793 + +2.0 162,793 + -0.2 162,892 + -0.0 163,043 + copD JDDGAC_00980 0.26 +1.0 163,198 - copD JDDGAC_00980 0.44 +0.9 163,263 + copD JDDGAC_00980 0.51 +0.5
Or see this region's nucleotide sequence