Experiment: WM_JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ureD1 and JDDGAC_07895 are separated by 380 nucleotides JDDGAC_07895 and opgE are separated by 224 nucleotides opgE and dgkA are separated by 92 nucleotides dgkA and tnp-IS3 are separated by 391 nucleotides
JDDGAC_07890: ureD1 - Putative urease accessory UreD-like protein, at 1,362,939 to 1,363,637
ureD1
JDDGAC_07895: JDDGAC_07895 - hypothetical protein, at 1,364,018 to 1,364,293
_07895
JDDGAC_07900: opgE - phosphoethanolamine transferase, at 1,364,518 to 1,366,137
opgE
JDDGAC_07905: dgkA - Diacylglycerol kinase, at 1,366,230 to 1,366,589
dgkA
JDDGAC_07910: tnp-IS3 - IS3 family transposase, at 1,366,981 to 1,367,868
tnp-IS3
Position (kb)
1364
1365
1366
1367 Strain fitness (log2 ratio)
-1
0
1
2 at 1363.784 kb on - strand at 1363.829 kb on + strand at 1363.830 kb on - strand at 1363.907 kb on + strand at 1363.940 kb on + strand at 1363.960 kb on - strand at 1363.971 kb on - strand at 1364.080 kb on + strand, within JDDGAC_07895 at 1364.128 kb on + strand, within JDDGAC_07895 at 1364.137 kb on + strand, within JDDGAC_07895 at 1365.353 kb on + strand, within opgE at 1365.519 kb on + strand, within opgE at 1366.028 kb on + strand at 1366.117 kb on - strand at 1366.610 kb on + strand at 1366.847 kb on + strand at 1366.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK32 remove 1,363,784 - -1.6 1,363,829 + -0.1 1,363,830 - -0.2 1,363,907 + +0.8 1,363,940 + +0.1 1,363,960 - -0.2 1,363,971 - +2.0 1,364,080 + JDDGAC_07895 0.22 -0.3 1,364,128 + JDDGAC_07895 0.40 -1.0 1,364,137 + JDDGAC_07895 0.43 +0.5 1,365,353 + opgE JDDGAC_07900 0.52 -0.5 1,365,519 + opgE JDDGAC_07900 0.62 -1.5 1,366,028 + +2.0 1,366,117 - +0.5 1,366,610 + -1.6 1,366,847 + +1.1 1,366,848 - +0.7
Or see this region's nucleotide sequence