Strain Fitness in Escherichia coli ECRC98 around JDDGAC_02335

Experiment: WM_JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmlc and bioD are separated by 124 nucleotidesbioD and clcB overlap by 48 nucleotides JDDGAC_02330: mlc - DNA-binding transcriptional repressor Mlc, at 422,188 to 423,408 mlc JDDGAC_02335: bioD - dethiobiotin synthase, at 423,533 to 424,228 bioD JDDGAC_02340: clcB - voltage-gated ClC-type chloride channel ClcB, at 424,181 to 425,437 clcB Position (kb) 423 424 425Strain fitness (log2 ratio) -2 -1 0 1 2 3at 422.922 kb on - strand, within mlcat 422.949 kb on + strand, within mlcat 422.949 kb on + strand, within mlcat 423.152 kb on + strand, within mlcat 423.182 kb on + strand, within mlcat 423.235 kb on - strand, within mlcat 423.258 kb on + strand, within mlcat 423.287 kb on - strandat 423.324 kb on + strandat 423.471 kb on - strandat 423.489 kb on - strandat 423.504 kb on - strandat 423.604 kb on - strand, within bioDat 423.748 kb on + strand, within bioDat 423.748 kb on + strand, within bioDat 423.749 kb on - strand, within bioDat 423.886 kb on + strand, within bioDat 424.137 kb on - strand, within bioDat 424.172 kb on + strandat 424.182 kb on + strandat 424.183 kb on - strandat 424.183 kb on - strandat 424.186 kb on + strandat 424.213 kb on + strandat 424.213 kb on + strandat 424.281 kb on - strandat 424.424 kb on - strand, within clcBat 424.431 kb on + strand, within clcBat 424.461 kb on - strand, within clcBat 424.461 kb on - strand, within clcBat 424.461 kb on - strand, within clcBat 424.584 kb on - strand, within clcBat 424.593 kb on + strand, within clcBat 424.808 kb on + strand, within clcBat 424.808 kb on + strand, within clcBat 424.809 kb on - strand, within clcBat 424.877 kb on - strand, within clcBat 424.907 kb on - strand, within clcBat 425.108 kb on + strand, within clcB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_JK42
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422,922 - mlc JDDGAC_02330 0.60 -1.0
422,949 + mlc JDDGAC_02330 0.62 +0.5
422,949 + mlc JDDGAC_02330 0.62 -0.3
423,152 + mlc JDDGAC_02330 0.79 +0.8
423,182 + mlc JDDGAC_02330 0.81 +0.9
423,235 - mlc JDDGAC_02330 0.86 -0.4
423,258 + mlc JDDGAC_02330 0.88 -2.4
423,287 - -0.8
423,324 + +0.4
423,471 - +3.0
423,489 - -1.1
423,504 - -1.1
423,604 - bioD JDDGAC_02335 0.10 -1.5
423,748 + bioD JDDGAC_02335 0.31 +0.0
423,748 + bioD JDDGAC_02335 0.31 -2.3
423,749 - bioD JDDGAC_02335 0.31 +0.7
423,886 + bioD JDDGAC_02335 0.51 -1.4
424,137 - bioD JDDGAC_02335 0.87 +1.8
424,172 + -0.6
424,182 + -2.4
424,183 - +0.2
424,183 - -0.7
424,186 + -0.0
424,213 + -0.5
424,213 + +0.2
424,281 - -2.0
424,424 - clcB JDDGAC_02340 0.19 -1.0
424,431 + clcB JDDGAC_02340 0.20 +1.1
424,461 - clcB JDDGAC_02340 0.22 -0.1
424,461 - clcB JDDGAC_02340 0.22 -0.1
424,461 - clcB JDDGAC_02340 0.22 -1.8
424,584 - clcB JDDGAC_02340 0.32 -0.9
424,593 + clcB JDDGAC_02340 0.33 +1.2
424,808 + clcB JDDGAC_02340 0.50 +0.4
424,808 + clcB JDDGAC_02340 0.50 -0.7
424,809 - clcB JDDGAC_02340 0.50 -0.6
424,877 - clcB JDDGAC_02340 0.55 -0.5
424,907 - clcB JDDGAC_02340 0.58 +2.3
425,108 + clcB JDDGAC_02340 0.74 -0.1

Or see this region's nucleotide sequence