Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28535

Experiment: WM_JK42

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzy and wcaA overlap by 32 nucleotideswcaA and wzx overlap by 14 nucleotideswzx and rfbE are separated by 2 nucleotides JDDGAC_28525: wzy - O157 family O-antigen polymerase, at 5,472,741 to 5,473,925 wzy JDDGAC_28530: wcaA - glycosyl transferase, at 5,473,894 to 5,474,640 wcaA JDDGAC_28535: wzx - O157 family O-antigen flippase, at 5,474,627 to 5,476,018 wzx JDDGAC_28540: rfbE - GDP-perosamine synthase RfbE/PerA, at 5,476,021 to 5,477,115 rfbE Position (kb) 5474 5475 5476 5477Strain fitness (log2 ratio) -3 -2 -1 0 1at 5473.841 kb on - strandat 5476.176 kb on + strand, within rfbEat 5476.575 kb on + strand, within rfbEat 5476.725 kb on + strand, within rfbE

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_JK42
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5,473,841 - -1.9
5,476,176 + rfbE JDDGAC_28540 0.14 -3.6
5,476,575 + rfbE JDDGAC_28540 0.51 -2.6
5,476,725 + rfbE JDDGAC_28540 0.64 -1.5

Or see this region's nucleotide sequence