Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28545

Experiment: TP2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfbE and rfaB are separated by 24 nucleotidesrfaB and JDDGAC_28550 are separated by 19 nucleotides JDDGAC_28540: rfbE - GDP-perosamine synthase RfbE/PerA, at 5,476,021 to 5,477,115 rfbE JDDGAC_28545: rfaB - glycosyl transferase, at 5,477,140 to 5,478,354 rfaB JDDGAC_28550: JDDGAC_28550 - GDP-mannose 4,6-dehydratase, at 5,478,374 to 5,479,492 _28550 Position (kb) 5477 5478 5479Strain fitness (log2 ratio) -3 -2 -1 0 1at 5476.176 kb on + strand, within rfbEat 5476.575 kb on + strand, within rfbEat 5476.725 kb on + strand, within rfbEat 5478.146 kb on + strand, within rfaBat 5478.860 kb on + strand, within JDDGAC_28550at 5479.304 kb on + strand, within JDDGAC_28550

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Per-strain Table

Position Strand Gene LocusTag Fraction TP2
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5,476,176 + rfbE JDDGAC_28540 0.14 -3.5
5,476,575 + rfbE JDDGAC_28540 0.51 -2.5
5,476,725 + rfbE JDDGAC_28540 0.64 -1.4
5,478,146 + rfaB JDDGAC_28545 0.83 -1.8
5,478,860 + JDDGAC_28550 0.43 +0.9
5,479,304 + JDDGAC_28550 0.83 -0.8

Or see this region's nucleotide sequence