Strain Fitness in Escherichia coli ECRC98 around JDDGAC_05535

Experiment: WM_TP11

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_05515 and JDDGAC_05520 are separated by 12 nucleotidesJDDGAC_05520 and JDDGAC_05525 are separated by 1 nucleotidesJDDGAC_05525 and JDDGAC_05530 overlap by 4 nucleotidesJDDGAC_05530 and JDDGAC_05535 are separated by 4 nucleotidesJDDGAC_05535 and gam are separated by 11 nucleotidesgam and JDDGAC_05545 are separated by 97 nucleotides JDDGAC_05515: JDDGAC_05515 - hypothetical protein, at 971,386 to 971,607 _05515 JDDGAC_05520: JDDGAC_05520 - hypothetical protein, at 971,620 to 971,874 _05520 JDDGAC_05525: JDDGAC_05525 - host nuclease inhibitor protein, at 971,876 to 972,157 _05525 JDDGAC_05530: JDDGAC_05530 - hypothetical protein, at 972,154 to 972,426 _05530 JDDGAC_05535: JDDGAC_05535 - hypothetical protein, at 972,431 to 972,724 _05535 JDDGAC_05540: gam - host-nuclease inhibitor protein Gam, at 972,736 to 973,266 gam JDDGAC_05545: JDDGAC_05545 - hypothetical protein, at 973,364 to 973,906 _05545 Position (kb) 972 973Strain fitness (log2 ratio) -2 -1 0 1 2at 971.618 kb on - strandat 971.625 kb on + strandat 971.958 kb on + strand, within JDDGAC_05525at 972.184 kb on + strand, within JDDGAC_05530at 972.248 kb on + strand, within JDDGAC_05530at 972.393 kb on + strand, within JDDGAC_05530at 972.550 kb on + strand, within JDDGAC_05535at 972.592 kb on + strand, within JDDGAC_05535at 972.700 kb on + strandat 972.701 kb on - strandat 973.206 kb on + strand, within gamat 973.305 kb on - strandat 973.349 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP11
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971,618 - -0.1
971,625 + -1.0
971,958 + JDDGAC_05525 0.29 +0.7
972,184 + JDDGAC_05530 0.11 -0.4
972,248 + JDDGAC_05530 0.34 +2.1
972,393 + JDDGAC_05530 0.88 +1.4
972,550 + JDDGAC_05535 0.40 +0.4
972,592 + JDDGAC_05535 0.55 -2.7
972,700 + -0.9
972,701 - -0.5
973,206 + gam JDDGAC_05540 0.89 +0.0
973,305 - +0.3
973,349 + -0.5

Or see this region's nucleotide sequence