Experiment: GB_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgm and seqA are separated by 25 nucleotides seqA and ybfF are separated by 184 nucleotides ybfF and ybfE are separated by 70 nucleotides ybfE and fldA are separated by 139 nucleotides
MCAODC_26315: pgm - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 1,985,571 to 1,987,211
pgm
MCAODC_26320: seqA - replication initiation negative regulator SeqA, at 1,987,237 to 1,987,782
seqA
MCAODC_26325: ybfF - esterase, at 1,987,967 to 1,988,731
ybfF
MCAODC_26330: ybfE - LexA regulated protein, at 1,988,802 to 1,989,164
ybfE
MCAODC_26335: fldA - flavodoxin FldA, at 1,989,304 to 1,989,834
fldA
Position (kb)
1987
1988
1989 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1986.967 kb on + strand, within pgm at 1986.967 kb on + strand, within pgm at 1986.967 kb on + strand, within pgm at 1986.967 kb on + strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1986.968 kb on - strand, within pgm at 1987.044 kb on - strand, within pgm at 1987.044 kb on - strand, within pgm at 1987.128 kb on + strand at 1987.128 kb on + strand at 1987.132 kb on + strand at 1987.132 kb on + strand at 1987.132 kb on + strand at 1987.132 kb on + strand at 1987.132 kb on + strand at 1987.132 kb on + strand at 1987.133 kb on - strand at 1987.133 kb on - strand at 1987.133 kb on - strand at 1987.133 kb on - strand at 1987.133 kb on - strand at 1987.133 kb on - strand at 1987.133 kb on - strand at 1987.133 kb on - strand at 1987.135 kb on + strand at 1987.135 kb on + strand at 1987.239 kb on - strand at 1987.243 kb on - strand at 1987.812 kb on - strand at 1987.812 kb on - strand at 1987.812 kb on - strand at 1987.828 kb on - strand at 1987.828 kb on - strand at 1987.828 kb on - strand at 1987.828 kb on - strand at 1987.828 kb on - strand at 1987.828 kb on - strand at 1987.836 kb on - strand at 1987.894 kb on - strand at 1987.927 kb on + strand at 1987.927 kb on + strand at 1987.927 kb on + strand at 1987.928 kb on - strand at 1987.928 kb on - strand at 1987.928 kb on - strand at 1987.928 kb on - strand at 1987.928 kb on - strand at 1987.930 kb on + strand at 1987.931 kb on - strand at 1988.012 kb on - strand at 1988.012 kb on - strand at 1988.098 kb on + strand, within ybfF at 1988.098 kb on + strand, within ybfF at 1988.099 kb on - strand, within ybfF at 1988.099 kb on - strand, within ybfF at 1988.099 kb on - strand, within ybfF at 1988.113 kb on + strand, within ybfF at 1988.113 kb on + strand, within ybfF at 1988.114 kb on - strand, within ybfF at 1988.186 kb on - strand, within ybfF at 1988.221 kb on + strand, within ybfF at 1988.261 kb on - strand, within ybfF at 1988.287 kb on + strand, within ybfF at 1988.328 kb on + strand, within ybfF at 1988.328 kb on + strand, within ybfF at 1988.328 kb on + strand, within ybfF at 1988.329 kb on - strand, within ybfF at 1988.410 kb on - strand, within ybfF at 1988.425 kb on + strand, within ybfF at 1988.485 kb on + strand, within ybfF at 1988.518 kb on + strand, within ybfF at 1988.518 kb on + strand, within ybfF at 1988.519 kb on - strand, within ybfF at 1988.519 kb on - strand, within ybfF at 1988.588 kb on + strand, within ybfF at 1988.589 kb on - strand, within ybfF at 1988.589 kb on - strand, within ybfF at 1988.616 kb on + strand, within ybfF at 1988.701 kb on - strand at 1988.718 kb on - strand at 1988.730 kb on - strand at 1988.742 kb on + strand at 1988.805 kb on + strand at 1988.805 kb on + strand at 1988.805 kb on + strand at 1988.806 kb on - strand at 1988.806 kb on - strand at 1988.806 kb on - strand at 1988.806 kb on - strand at 1988.808 kb on + strand at 1988.895 kb on - strand, within ybfE at 1988.895 kb on - strand, within ybfE at 1988.902 kb on + strand, within ybfE at 1988.902 kb on + strand, within ybfE at 1988.902 kb on + strand, within ybfE at 1988.902 kb on + strand, within ybfE at 1988.902 kb on + strand, within ybfE at 1988.903 kb on - strand, within ybfE at 1988.903 kb on - strand, within ybfE at 1988.975 kb on + strand, within ybfE at 1988.976 kb on - strand, within ybfE at 1988.976 kb on - strand, within ybfE at 1988.978 kb on + strand, within ybfE at 1988.978 kb on + strand, within ybfE at 1988.979 kb on - strand, within ybfE at 1989.010 kb on + strand, within ybfE at 1989.010 kb on + strand, within ybfE at 1989.011 kb on - strand, within ybfE at 1989.090 kb on - strand, within ybfE at 1989.090 kb on - strand, within ybfE at 1989.122 kb on - strand, within ybfE at 1989.146 kb on + strand at 1989.147 kb on - strand at 1989.163 kb on - strand at 1989.180 kb on + strand at 1989.180 kb on + strand at 1989.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_Bas14 remove 1,986,967 + pgm MCAODC_26315 0.85 -0.0 1,986,967 + pgm MCAODC_26315 0.85 +1.8 1,986,967 + pgm MCAODC_26315 0.85 -1.6 1,986,967 + pgm MCAODC_26315 0.85 -0.7 1,986,968 - pgm MCAODC_26315 0.85 -0.4 1,986,968 - pgm MCAODC_26315 0.85 -0.3 1,986,968 - pgm MCAODC_26315 0.85 -1.7 1,986,968 - pgm MCAODC_26315 0.85 -0.2 1,986,968 - pgm MCAODC_26315 0.85 -0.2 1,986,968 - pgm MCAODC_26315 0.85 +0.0 1,986,968 - pgm MCAODC_26315 0.85 -0.8 1,986,968 - pgm MCAODC_26315 0.85 +1.1 1,986,968 - pgm MCAODC_26315 0.85 -0.8 1,987,044 - pgm MCAODC_26315 0.90 +1.4 1,987,044 - pgm MCAODC_26315 0.90 -1.3 1,987,128 + -0.1 1,987,128 + +0.9 1,987,132 + -0.0 1,987,132 + -1.0 1,987,132 + -0.5 1,987,132 + +0.1 1,987,132 + -0.2 1,987,132 + -1.4 1,987,133 - +0.2 1,987,133 - -0.5 1,987,133 - -0.1 1,987,133 - -2.6 1,987,133 - +0.7 1,987,133 - -0.1 1,987,133 - -2.4 1,987,133 - -0.7 1,987,135 + -1.0 1,987,135 + -3.0 1,987,239 - -0.6 1,987,243 - -0.6 1,987,812 - -0.2 1,987,812 - -0.7 1,987,812 - +0.8 1,987,828 - -1.2 1,987,828 - -0.3 1,987,828 - +1.6 1,987,828 - +0.4 1,987,828 - +0.7 1,987,828 - -0.4 1,987,836 - -1.4 1,987,894 - -0.2 1,987,927 + +1.0 1,987,927 + -4.0 1,987,927 + -1.9 1,987,928 - +0.7 1,987,928 - +0.2 1,987,928 - -0.5 1,987,928 - +0.5 1,987,928 - -0.6 1,987,930 + -2.4 1,987,931 - +1.3 1,988,012 - -1.4 1,988,012 - -0.9 1,988,098 + ybfF MCAODC_26325 0.17 -1.1 1,988,098 + ybfF MCAODC_26325 0.17 -2.0 1,988,099 - ybfF MCAODC_26325 0.17 +1.6 1,988,099 - ybfF MCAODC_26325 0.17 -1.8 1,988,099 - ybfF MCAODC_26325 0.17 -0.8 1,988,113 + ybfF MCAODC_26325 0.19 +0.4 1,988,113 + ybfF MCAODC_26325 0.19 +0.3 1,988,114 - ybfF MCAODC_26325 0.19 -0.9 1,988,186 - ybfF MCAODC_26325 0.29 +0.3 1,988,221 + ybfF MCAODC_26325 0.33 -1.4 1,988,261 - ybfF MCAODC_26325 0.38 -0.1 1,988,287 + ybfF MCAODC_26325 0.42 -1.4 1,988,328 + ybfF MCAODC_26325 0.47 -1.5 1,988,328 + ybfF MCAODC_26325 0.47 -2.9 1,988,328 + ybfF MCAODC_26325 0.47 -1.1 1,988,329 - ybfF MCAODC_26325 0.47 -0.6 1,988,410 - ybfF MCAODC_26325 0.58 -1.7 1,988,425 + ybfF MCAODC_26325 0.60 -2.5 1,988,485 + ybfF MCAODC_26325 0.68 -1.2 1,988,518 + ybfF MCAODC_26325 0.72 -0.4 1,988,518 + ybfF MCAODC_26325 0.72 -0.3 1,988,519 - ybfF MCAODC_26325 0.72 -3.0 1,988,519 - ybfF MCAODC_26325 0.72 +0.4 1,988,588 + ybfF MCAODC_26325 0.81 +2.1 1,988,589 - ybfF MCAODC_26325 0.81 -2.3 1,988,589 - ybfF MCAODC_26325 0.81 -1.3 1,988,616 + ybfF MCAODC_26325 0.85 -0.7 1,988,701 - +0.1 1,988,718 - -0.9 1,988,730 - +2.6 1,988,742 + +0.6 1,988,805 + +0.0 1,988,805 + -1.6 1,988,805 + +0.1 1,988,806 - +0.4 1,988,806 - -1.9 1,988,806 - -0.4 1,988,806 - -0.5 1,988,808 + -1.7 1,988,895 - ybfE MCAODC_26330 0.26 -2.5 1,988,895 - ybfE MCAODC_26330 0.26 +0.7 1,988,902 + ybfE MCAODC_26330 0.28 -1.6 1,988,902 + ybfE MCAODC_26330 0.28 +0.6 1,988,902 + ybfE MCAODC_26330 0.28 -1.2 1,988,902 + ybfE MCAODC_26330 0.28 -0.9 1,988,902 + ybfE MCAODC_26330 0.28 -1.8 1,988,903 - ybfE MCAODC_26330 0.28 -0.2 1,988,903 - ybfE MCAODC_26330 0.28 -0.1 1,988,975 + ybfE MCAODC_26330 0.48 -0.8 1,988,976 - ybfE MCAODC_26330 0.48 +1.6 1,988,976 - ybfE MCAODC_26330 0.48 -2.8 1,988,978 + ybfE MCAODC_26330 0.48 +0.1 1,988,978 + ybfE MCAODC_26330 0.48 +1.2 1,988,979 - ybfE MCAODC_26330 0.49 -1.0 1,989,010 + ybfE MCAODC_26330 0.57 -0.7 1,989,010 + ybfE MCAODC_26330 0.57 +1.9 1,989,011 - ybfE MCAODC_26330 0.58 -0.6 1,989,090 - ybfE MCAODC_26330 0.79 -0.8 1,989,090 - ybfE MCAODC_26330 0.79 -1.6 1,989,122 - ybfE MCAODC_26330 0.88 -0.8 1,989,146 + -0.4 1,989,147 - -0.1 1,989,163 - +1.2 1,989,180 + +0.7 1,989,180 + +0.9 1,989,235 + +0.8
Or see this region's nucleotide sequence