Experiment: GB_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lhr and grxD are separated by 50 nucleotides grxD and mepH are separated by 334 nucleotides mepH and sodB are separated by 127 nucleotides sodB and ydhP are separated by 145 nucleotides
MCAODC_17990: lhr - ATP-dependent helicase, at 458,082 to 462,698
lhr
MCAODC_17995: grxD - monothiol glutaredoxin 4, at 462,749 to 463,096
grxD
MCAODC_18000: mepH - peptidoglycan DD-endopeptidase MepH, at 463,431 to 464,246
mepH
MCAODC_18005: sodB - superoxide dismutase [Fe], at 464,374 to 464,955
sodB
MCAODC_18010: ydhP - Inner membrane transport protein YdhP, at 465,101 to 466,270
ydhP
Position (kb) 463
464
465 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 462.472 kb on + strand at 462.478 kb on - strand at 462.594 kb on - strand at 462.777 kb on + strand at 462.778 kb on - strand at 462.778 kb on - strand at 462.778 kb on - strand at 462.778 kb on - strand at 462.842 kb on - strand, within grxD at 462.899 kb on - strand, within grxD at 462.904 kb on - strand, within grxD at 462.952 kb on - strand, within grxD at 462.952 kb on - strand, within grxD at 463.121 kb on - strand at 463.129 kb on - strand at 463.142 kb on - strand at 463.340 kb on - strand at 463.340 kb on - strand at 463.343 kb on - strand at 463.358 kb on + strand at 463.358 kb on + strand at 463.359 kb on - strand at 463.365 kb on + strand at 463.366 kb on - strand at 463.403 kb on + strand at 463.492 kb on + strand at 463.547 kb on + strand, within mepH at 463.547 kb on + strand, within mepH at 463.625 kb on + strand, within mepH at 463.685 kb on + strand, within mepH at 463.868 kb on + strand, within mepH at 463.959 kb on + strand, within mepH at 463.961 kb on + strand, within mepH at 464.007 kb on + strand, within mepH at 464.007 kb on + strand, within mepH at 464.039 kb on + strand, within mepH at 464.039 kb on + strand, within mepH at 464.039 kb on + strand, within mepH at 464.039 kb on + strand, within mepH at 464.113 kb on - strand, within mepH at 464.276 kb on + strand at 464.330 kb on - strand at 464.340 kb on + strand at 464.341 kb on - strand at 464.344 kb on + strand at 464.344 kb on + strand at 464.344 kb on + strand at 464.344 kb on + strand at 464.345 kb on - strand at 464.354 kb on - strand at 464.370 kb on - strand at 464.396 kb on + strand at 464.401 kb on + strand at 464.401 kb on + strand at 464.449 kb on + strand, within sodB at 464.476 kb on + strand, within sodB at 464.477 kb on - strand, within sodB at 464.477 kb on - strand, within sodB at 464.477 kb on - strand, within sodB at 464.485 kb on - strand, within sodB at 464.502 kb on + strand, within sodB at 464.524 kb on - strand, within sodB at 464.860 kb on + strand, within sodB at 464.863 kb on + strand, within sodB at 464.863 kb on + strand, within sodB at 464.864 kb on - strand, within sodB at 464.864 kb on - strand, within sodB at 464.893 kb on + strand, within sodB at 464.953 kb on + strand at 464.956 kb on + strand at 464.975 kb on + strand at 464.996 kb on + strand at 465.041 kb on + strand at 465.102 kb on + strand at 465.103 kb on - strand at 465.103 kb on - strand at 465.103 kb on - strand at 465.103 kb on - strand at 465.149 kb on - strand at 465.149 kb on - strand at 465.149 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction GB_Bas14 remove 462,472 + -0.4 462,478 - +2.4 462,594 - -0.6 462,777 + +1.7 462,778 - +0.9 462,778 - +0.8 462,778 - +0.6 462,778 - -3.0 462,842 - grxD MCAODC_17995 0.27 -0.5 462,899 - grxD MCAODC_17995 0.43 +0.3 462,904 - grxD MCAODC_17995 0.45 -1.0 462,952 - grxD MCAODC_17995 0.58 +1.9 462,952 - grxD MCAODC_17995 0.58 -1.5 463,121 - -0.1 463,129 - -0.5 463,142 - +0.2 463,340 - +1.7 463,340 - +1.5 463,343 - +1.0 463,358 + -0.0 463,358 + -0.1 463,359 - -0.3 463,365 + +0.3 463,366 - +1.1 463,403 + -0.9 463,492 + +0.1 463,547 + mepH MCAODC_18000 0.14 +1.1 463,547 + mepH MCAODC_18000 0.14 -2.5 463,625 + mepH MCAODC_18000 0.24 -1.2 463,685 + mepH MCAODC_18000 0.31 +0.1 463,868 + mepH MCAODC_18000 0.54 +0.1 463,959 + mepH MCAODC_18000 0.65 -0.7 463,961 + mepH MCAODC_18000 0.65 +0.7 464,007 + mepH MCAODC_18000 0.71 -2.0 464,007 + mepH MCAODC_18000 0.71 +0.5 464,039 + mepH MCAODC_18000 0.75 -2.0 464,039 + mepH MCAODC_18000 0.75 +0.3 464,039 + mepH MCAODC_18000 0.75 -1.2 464,039 + mepH MCAODC_18000 0.75 +0.5 464,113 - mepH MCAODC_18000 0.84 -1.0 464,276 + -0.8 464,330 - +1.0 464,340 + +0.7 464,341 - +1.0 464,344 + +0.4 464,344 + -1.5 464,344 + +0.0 464,344 + +0.5 464,345 - -0.9 464,354 - -0.5 464,370 - -0.9 464,396 + -1.5 464,401 + +0.5 464,401 + +2.3 464,449 + sodB MCAODC_18005 0.13 -0.5 464,476 + sodB MCAODC_18005 0.18 +0.8 464,477 - sodB MCAODC_18005 0.18 -0.5 464,477 - sodB MCAODC_18005 0.18 +0.1 464,477 - sodB MCAODC_18005 0.18 -0.2 464,485 - sodB MCAODC_18005 0.19 -0.0 464,502 + sodB MCAODC_18005 0.22 -1.2 464,524 - sodB MCAODC_18005 0.26 +1.3 464,860 + sodB MCAODC_18005 0.84 -1.8 464,863 + sodB MCAODC_18005 0.84 -1.1 464,863 + sodB MCAODC_18005 0.84 -0.2 464,864 - sodB MCAODC_18005 0.84 -0.7 464,864 - sodB MCAODC_18005 0.84 -2.8 464,893 + sodB MCAODC_18005 0.89 -0.7 464,953 + -1.3 464,956 + -0.2 464,975 + -1.1 464,996 + -0.5 465,041 + +0.1 465,102 + +0.7 465,103 - +1.0 465,103 - +1.2 465,103 - +0.2 465,103 - -1.8 465,149 - -0.4 465,149 - -1.2 465,149 - +1.2
Or see this region's nucleotide sequence