Experiment: GB_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlhA and yncJ are separated by 91 nucleotides yncJ and hicA are separated by 281 nucleotides hicA and hicB are separated by 24 nucleotides hicB and ydcR are separated by 78 nucleotides
MCAODC_16340: rlhA - 23S rRNA 5-hydroxycytidine C2501 synthase, at 160,066 to 162,069
rlhA
MCAODC_16345: yncJ - Uncharacterized protein YncJ, at 162,161 to 162,331
yncJ
MCAODC_16350: hicA - type II toxin-antitoxin system mRNA interferase toxin HicA, at 162,613 to 162,789
hicA
MCAODC_16355: hicB - type II toxin-antitoxin system antitoxin HicB, at 162,814 to 163,251
hicB
MCAODC_16360: ydcR - Uncharacterized HTH-type transcriptional regulator YdcR, at 163,330 to 164,736
ydcR
Position (kb) 162
163
164 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 161.918 kb on + strand at 161.919 kb on - strand at 161.921 kb on + strand at 161.927 kb on + strand at 162.531 kb on + strand at 162.532 kb on - strand at 162.571 kb on + strand at 162.662 kb on + strand, within hicA at 162.741 kb on + strand, within hicA at 162.877 kb on + strand, within hicB at 162.879 kb on + strand, within hicB at 163.073 kb on + strand, within hicB at 163.168 kb on - strand, within hicB at 163.232 kb on + strand at 163.232 kb on + strand at 163.253 kb on + strand at 163.291 kb on + strand at 163.318 kb on + strand at 163.449 kb on + strand at 163.494 kb on + strand, within ydcR at 163.495 kb on - strand, within ydcR at 163.495 kb on - strand, within ydcR at 163.697 kb on + strand, within ydcR at 163.868 kb on + strand, within ydcR at 163.869 kb on - strand, within ydcR at 163.933 kb on + strand, within ydcR at 164.071 kb on - strand, within ydcR at 164.074 kb on - strand, within ydcR at 164.143 kb on + strand, within ydcR at 164.174 kb on - strand, within ydcR
Per-strain Table
Position Strand Gene LocusTag Fraction GB_Bas14 remove 161,918 + +0.9 161,919 - +0.3 161,921 + +1.3 161,927 + -1.3 162,531 + -1.1 162,532 - -0.1 162,571 + +0.6 162,662 + hicA MCAODC_16350 0.28 +2.3 162,741 + hicA MCAODC_16350 0.72 +2.8 162,877 + hicB MCAODC_16355 0.14 +0.2 162,879 + hicB MCAODC_16355 0.15 -1.7 163,073 + hicB MCAODC_16355 0.59 -0.1 163,168 - hicB MCAODC_16355 0.81 -1.2 163,232 + +0.6 163,232 + +0.1 163,253 + -0.5 163,291 + +2.5 163,318 + +0.8 163,449 + +3.3 163,494 + ydcR MCAODC_16360 0.12 +0.3 163,495 - ydcR MCAODC_16360 0.12 +0.5 163,495 - ydcR MCAODC_16360 0.12 -0.7 163,697 + ydcR MCAODC_16360 0.26 -1.7 163,868 + ydcR MCAODC_16360 0.38 +1.3 163,869 - ydcR MCAODC_16360 0.38 +1.8 163,933 + ydcR MCAODC_16360 0.43 +1.9 164,071 - ydcR MCAODC_16360 0.53 -2.1 164,074 - ydcR MCAODC_16360 0.53 +0.0 164,143 + ydcR MCAODC_16360 0.58 -0.4 164,174 - ydcR MCAODC_16360 0.60 +0.5
Or see this region's nucleotide sequence