Strain Fitness in Escherichia coli ECRC101 around MCAODC_14915

Experiment: GB_Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybcN and MCAODC_14905 overlap by 1 nucleotidesMCAODC_14905 and ybcO overlap by 8 nucleotidesybcO and rusA overlap by 4 nucleotidesrusA and ylcG overlap by 1 nucleotidesylcG and MCAODC_14925 overlap by 4 nucleotidesMCAODC_14925 and MCAODC_14930 are separated by 62 nucleotides MCAODC_14900: ybcN - DNA base-flipping protein YbcN, at 2,922,363 to 2,922,818 ybcN MCAODC_14905: MCAODC_14905 - Prophage protein NinE, at 2,922,818 to 2,922,988 _14905 MCAODC_14910: ybcO - Putative nuclease YbcO, at 2,922,981 to 2,923,271 ybcO MCAODC_14915: rusA - crossover junction endodeoxyribonuclease RusA, at 2,923,268 to 2,923,630 rusA MCAODC_14920: ylcG - Uncharacterized protein YlcG, at 2,923,630 to 2,923,767 ylcG MCAODC_14925: MCAODC_14925 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 2,923,764 to 2,923,790 _14925 MCAODC_14930: MCAODC_14930 - antitermination protein, at 2,923,853 to 2,924,287 _14930 Position (kb) 2923 2924Strain fitness (log2 ratio) -1 0 1at 2922.543 kb on + strand, within ybcNat 2922.543 kb on + strand, within ybcNat 2922.587 kb on - strand, within ybcNat 2923.262 kb on + strandat 2923.273 kb on + strandat 2923.310 kb on - strand, within rusAat 2923.310 kb on - strand, within rusA

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_Bas14
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2,922,543 + ybcN MCAODC_14900 0.39 -0.0
2,922,543 + ybcN MCAODC_14900 0.39 -0.2
2,922,587 - ybcN MCAODC_14900 0.49 +1.4
2,923,262 + -0.3
2,923,273 + -0.8
2,923,310 - rusA MCAODC_14915 0.12 -0.8
2,923,310 - rusA MCAODC_14915 0.12 -1.6

Or see this region's nucleotide sequence