Experiment: GB_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcN and MCAODC_14905 overlap by 1 nucleotides MCAODC_14905 and ybcO overlap by 8 nucleotides ybcO and rusA overlap by 4 nucleotides rusA and ylcG overlap by 1 nucleotides ylcG and MCAODC_14925 overlap by 4 nucleotides MCAODC_14925 and MCAODC_14930 are separated by 62 nucleotides
MCAODC_14900: ybcN - DNA base-flipping protein YbcN, at 2,922,363 to 2,922,818
ybcN
MCAODC_14905: MCAODC_14905 - Prophage protein NinE, at 2,922,818 to 2,922,988
_14905
MCAODC_14910: ybcO - Putative nuclease YbcO, at 2,922,981 to 2,923,271
ybcO
MCAODC_14915: rusA - crossover junction endodeoxyribonuclease RusA, at 2,923,268 to 2,923,630
rusA
MCAODC_14920: ylcG - Uncharacterized protein YlcG, at 2,923,630 to 2,923,767
ylcG
MCAODC_14925: MCAODC_14925 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 2,923,764 to 2,923,790
_14925
MCAODC_14930: MCAODC_14930 - antitermination protein, at 2,923,853 to 2,924,287
_14930
Position (kb)
2923
2924 Strain fitness (log2 ratio)
-1
0
1 at 2922.543 kb on + strand, within ybcN at 2922.543 kb on + strand, within ybcN at 2922.587 kb on - strand, within ybcN at 2923.262 kb on + strand at 2923.273 kb on + strand at 2923.310 kb on - strand, within rusA at 2923.310 kb on - strand, within rusA
Per-strain Table
Position Strand Gene LocusTag Fraction GB_Bas14 remove 2,922,543 + ybcN MCAODC_14900 0.39 -0.0 2,922,543 + ybcN MCAODC_14900 0.39 -0.2 2,922,587 - ybcN MCAODC_14900 0.49 +1.4 2,923,262 + -0.3 2,923,273 + -0.8 2,923,310 - rusA MCAODC_14915 0.12 -0.8 2,923,310 - rusA MCAODC_14915 0.12 -1.6
Or see this region's nucleotide sequence