Strain Fitness in Escherichia coli ECRC101 around MCAODC_08625

Experiment: GB_Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfMet_trna and rimP are separated by 206 nucleotidesrimP and nusA are separated by 27 nucleotidesnusA and infB are separated by 24 nucleotides MCAODC_08615: fMet_trna - tRNA-fMet(cat), at 1,725,408 to 1,725,484 _trna MCAODC_08620: rimP - ribosome maturation factor RimP, at 1,725,691 to 1,726,143 rimP MCAODC_08625: nusA - Transcription termination/antitermination protein NusA, at 1,726,171 to 1,727,658 nusA MCAODC_08630: infB - Translation initiation factor IF-2, at 1,727,683 to 1,730,355 infB Position (kb) 1726 1727 1728Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1725.179 kb on - strandat 1725.197 kb on + strandat 1725.207 kb on - strandat 1725.233 kb on - strandat 1725.310 kb on + strandat 1725.310 kb on + strandat 1725.313 kb on - strandat 1725.328 kb on + strandat 1725.328 kb on + strandat 1725.361 kb on + strandat 1725.512 kb on + strandat 1725.601 kb on + strandat 1725.605 kb on + strandat 1727.451 kb on + strand, within nusAat 1727.489 kb on + strand, within nusAat 1727.532 kb on + strandat 1727.532 kb on + strandat 1727.532 kb on + strandat 1727.628 kb on + strandat 1727.666 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction GB_Bas14
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1,725,179 - -1.1
1,725,197 + +0.9
1,725,207 - +1.0
1,725,233 - +0.3
1,725,310 + -1.0
1,725,310 + +0.1
1,725,313 - -0.0
1,725,328 + +0.5
1,725,328 + -0.2
1,725,361 + +0.1
1,725,512 + -0.2
1,725,601 + +2.9
1,725,605 + -2.0
1,727,451 + nusA MCAODC_08625 0.86 -3.1
1,727,489 + nusA MCAODC_08625 0.89 -1.2
1,727,532 + -0.2
1,727,532 + -0.4
1,727,532 + +2.9
1,727,628 + -0.6
1,727,666 + +0.5

Or see this region's nucleotide sequence