Experiment: GB_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaG and rfaP overlap by 8 nucleotides rfaP and waaO are separated by 42 nucleotides waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides
MCAODC_06165: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 1,235,240 to 1,236,364
rfaG
MCAODC_06170: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 1,236,357 to 1,237,154
rfaP
MCAODC_06175: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,237,197 to 1,238,204
waaO
MCAODC_06180: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,238,230 to 1,238,937
rfaY
MCAODC_06185: rfaJ - glucosyltransferase, at 1,238,962 to 1,239,975
rfaJ
Position (kb) 1237
1238
1239 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1236.231 kb on + strand, within rfaG at 1236.309 kb on + strand at 1236.311 kb on + strand at 1236.312 kb on - strand at 1236.343 kb on - strand at 1236.368 kb on + strand at 1236.495 kb on + strand, within rfaP at 1236.568 kb on + strand, within rfaP at 1236.629 kb on + strand, within rfaP at 1236.629 kb on + strand, within rfaP at 1236.686 kb on + strand, within rfaP at 1236.686 kb on + strand, within rfaP at 1236.687 kb on - strand, within rfaP at 1236.715 kb on + strand, within rfaP at 1236.715 kb on + strand, within rfaP at 1236.782 kb on + strand, within rfaP at 1236.786 kb on - strand, within rfaP at 1236.846 kb on + strand, within rfaP at 1236.847 kb on - strand, within rfaP at 1236.847 kb on - strand, within rfaP at 1236.848 kb on + strand, within rfaP at 1236.849 kb on - strand, within rfaP at 1237.044 kb on + strand, within rfaP at 1237.152 kb on + strand at 1237.282 kb on + strand at 1237.324 kb on + strand, within waaO at 1237.490 kb on - strand, within waaO at 1237.512 kb on + strand, within waaO at 1237.570 kb on + strand, within waaO at 1237.720 kb on + strand, within waaO at 1237.732 kb on - strand, within waaO at 1237.918 kb on - strand, within waaO at 1237.920 kb on - strand, within waaO at 1237.981 kb on + strand, within waaO at 1238.087 kb on - strand, within waaO at 1238.211 kb on - strand at 1238.402 kb on + strand, within rfaY at 1238.673 kb on - strand, within rfaY at 1238.825 kb on - strand, within rfaY at 1238.859 kb on + strand, within rfaY at 1238.996 kb on + strand at 1239.045 kb on + strand at 1239.183 kb on - strand, within rfaJ
Per-strain Table
Position Strand Gene LocusTag Fraction GB_Bas14 remove 1,236,231 + rfaG MCAODC_06165 0.88 -2.1 1,236,309 + -0.4 1,236,311 + -1.8 1,236,312 - +2.4 1,236,343 - -0.8 1,236,368 + -0.5 1,236,495 + rfaP MCAODC_06170 0.17 +0.8 1,236,568 + rfaP MCAODC_06170 0.26 -0.9 1,236,629 + rfaP MCAODC_06170 0.34 +0.1 1,236,629 + rfaP MCAODC_06170 0.34 -0.0 1,236,686 + rfaP MCAODC_06170 0.41 -2.2 1,236,686 + rfaP MCAODC_06170 0.41 -0.5 1,236,687 - rfaP MCAODC_06170 0.41 -1.2 1,236,715 + rfaP MCAODC_06170 0.45 +1.0 1,236,715 + rfaP MCAODC_06170 0.45 +0.8 1,236,782 + rfaP MCAODC_06170 0.53 -0.2 1,236,786 - rfaP MCAODC_06170 0.54 +0.2 1,236,846 + rfaP MCAODC_06170 0.61 -2.0 1,236,847 - rfaP MCAODC_06170 0.61 -1.2 1,236,847 - rfaP MCAODC_06170 0.61 -1.6 1,236,848 + rfaP MCAODC_06170 0.62 -2.5 1,236,849 - rfaP MCAODC_06170 0.62 -1.1 1,237,044 + rfaP MCAODC_06170 0.86 +0.2 1,237,152 + -1.4 1,237,282 + -0.2 1,237,324 + waaO MCAODC_06175 0.13 -0.5 1,237,490 - waaO MCAODC_06175 0.29 +0.1 1,237,512 + waaO MCAODC_06175 0.31 -2.4 1,237,570 + waaO MCAODC_06175 0.37 -0.4 1,237,720 + waaO MCAODC_06175 0.52 -2.6 1,237,732 - waaO MCAODC_06175 0.53 -1.2 1,237,918 - waaO MCAODC_06175 0.72 -2.5 1,237,920 - waaO MCAODC_06175 0.72 -1.4 1,237,981 + waaO MCAODC_06175 0.78 -1.4 1,238,087 - waaO MCAODC_06175 0.88 +0.7 1,238,211 - -2.0 1,238,402 + rfaY MCAODC_06180 0.24 -0.0 1,238,673 - rfaY MCAODC_06180 0.63 +0.5 1,238,825 - rfaY MCAODC_06180 0.84 -0.4 1,238,859 + rfaY MCAODC_06180 0.89 -0.8 1,238,996 + -2.6 1,239,045 + -2.3 1,239,183 - rfaJ MCAODC_06185 0.22 -3.3
Or see this region's nucleotide sequence