Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fimB and JDDGAC_27650 are separated by 33 nucleotides JDDGAC_27650 and JDDGAC_27655 are separated by 18 nucleotides JDDGAC_27655 and JDDGAC_27660 are separated by 3 nucleotides JDDGAC_27660 and JDDGAC_27665 are separated by 87 nucleotides JDDGAC_27665 and JDDGAC_27670 overlap by 4 nucleotides JDDGAC_27670 and JDDGAC_27675 are separated by 333 nucleotides JDDGAC_27675 and JDDGAC_27680 are separated by 1 nucleotides
JDDGAC_27645: fimB - site-specific integrase, at 5,317,095 to 5,318,381
fimB
JDDGAC_27650: JDDGAC_27650 - excisionase, at 5,318,415 to 5,318,669
_27650
JDDGAC_27655: JDDGAC_27655 - Bacteriophage protein, at 5,318,688 to 5,318,822
_27655
JDDGAC_27660: JDDGAC_27660 - hypothetical protein, at 5,318,826 to 5,319,068
_27660
JDDGAC_27665: JDDGAC_27665 - DUF551 domain-containing protein, at 5,319,156 to 5,319,518
_27665
JDDGAC_27670: JDDGAC_27670 - Phage protein, at 5,319,515 to 5,319,871
_27670
JDDGAC_27675: JDDGAC_27675 - hypothetical protein, at 5,320,205 to 5,320,381
_27675
JDDGAC_27680: JDDGAC_27680 - Ead/Ea22-like protein, at 5,320,383 to 5,321,330
_27680
Position (kb) 5319
5320 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5318.312 kb on + strand at 5318.312 kb on + strand at 5318.312 kb on + strand at 5318.313 kb on - strand at 5318.313 kb on - strand at 5318.314 kb on + strand at 5318.314 kb on + strand at 5318.315 kb on - strand at 5318.326 kb on - strand at 5318.327 kb on + strand at 5318.328 kb on - strand at 5318.376 kb on - strand at 5318.488 kb on + strand, within JDDGAC_27650 at 5318.524 kb on - strand, within JDDGAC_27650 at 5318.524 kb on - strand, within JDDGAC_27650 at 5318.524 kb on - strand, within JDDGAC_27650 at 5318.681 kb on - strand at 5318.824 kb on + strand at 5318.825 kb on - strand at 5318.825 kb on - strand at 5318.825 kb on - strand at 5318.825 kb on - strand at 5318.896 kb on + strand, within JDDGAC_27660 at 5318.912 kb on + strand, within JDDGAC_27660 at 5318.913 kb on - strand, within JDDGAC_27660 at 5318.913 kb on - strand, within JDDGAC_27660 at 5319.074 kb on + strand at 5319.202 kb on - strand, within JDDGAC_27665 at 5319.266 kb on - strand, within JDDGAC_27665 at 5319.269 kb on - strand, within JDDGAC_27665 at 5319.269 kb on - strand, within JDDGAC_27665 at 5319.326 kb on - strand, within JDDGAC_27665
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 5,318,312 + -1.0 5,318,312 + +1.4 5,318,312 + -2.2 5,318,313 - -1.1 5,318,313 - +1.3 5,318,314 + -0.8 5,318,314 + +0.4 5,318,315 - -0.9 5,318,326 - +0.7 5,318,327 + +0.9 5,318,328 - -0.4 5,318,376 - +0.2 5,318,488 + JDDGAC_27650 0.29 +1.1 5,318,524 - JDDGAC_27650 0.43 -0.5 5,318,524 - JDDGAC_27650 0.43 -0.2 5,318,524 - JDDGAC_27650 0.43 -0.0 5,318,681 - -1.4 5,318,824 + +0.9 5,318,825 - -0.6 5,318,825 - +0.7 5,318,825 - -0.1 5,318,825 - -1.0 5,318,896 + JDDGAC_27660 0.29 -2.3 5,318,912 + JDDGAC_27660 0.35 +1.7 5,318,913 - JDDGAC_27660 0.36 -1.1 5,318,913 - JDDGAC_27660 0.36 +0.1 5,319,074 + +2.0 5,319,202 - JDDGAC_27665 0.13 +0.8 5,319,266 - JDDGAC_27665 0.30 +0.4 5,319,269 - JDDGAC_27665 0.31 -1.4 5,319,269 - JDDGAC_27665 0.31 -2.1 5,319,326 - JDDGAC_27665 0.47 -1.8
Or see this region's nucleotide sequence