Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dacB and greA are separated by 247 nucleotides greA and yhbY are separated by 155 nucleotides yhbY and rlmE are separated by 125 nucleotides
JDDGAC_21665: dacB - serine-type D-Ala-D-Ala carboxypeptidase, at 4,145,189 to 4,146,622
dacB
JDDGAC_21670: greA - transcription elongation factor GreA, at 4,146,870 to 4,147,346
greA
JDDGAC_21675: yhbY - ribosome assembly RNA-binding protein YhbY, at 4,147,502 to 4,147,795
yhbY
JDDGAC_21680: rlmE - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 4,147,921 to 4,148,550
rlmE
Position (kb)
4146
4147
4148 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4145.977 kb on - strand, within dacB at 4146.011 kb on + strand, within dacB at 4146.039 kb on + strand, within dacB at 4146.039 kb on + strand, within dacB at 4146.040 kb on - strand, within dacB at 4146.040 kb on - strand, within dacB at 4146.053 kb on + strand, within dacB at 4146.054 kb on - strand, within dacB at 4146.054 kb on - strand, within dacB at 4146.055 kb on + strand, within dacB at 4146.088 kb on - strand, within dacB at 4146.119 kb on + strand, within dacB at 4146.120 kb on - strand, within dacB at 4146.120 kb on - strand, within dacB at 4146.120 kb on - strand, within dacB at 4146.120 kb on - strand, within dacB at 4146.209 kb on - strand, within dacB at 4146.209 kb on - strand, within dacB at 4146.209 kb on - strand, within dacB at 4146.212 kb on + strand, within dacB at 4146.213 kb on - strand, within dacB at 4146.213 kb on - strand, within dacB at 4146.213 kb on - strand, within dacB at 4146.213 kb on - strand, within dacB at 4146.321 kb on - strand, within dacB at 4146.321 kb on - strand, within dacB at 4146.331 kb on + strand, within dacB at 4146.335 kb on + strand, within dacB at 4146.468 kb on + strand, within dacB at 4146.469 kb on - strand, within dacB at 4146.541 kb on + strand at 4146.541 kb on + strand at 4146.546 kb on + strand at 4146.546 kb on + strand at 4146.546 kb on + strand at 4146.547 kb on - strand at 4146.547 kb on - strand at 4146.581 kb on + strand at 4146.582 kb on - strand at 4146.582 kb on - strand at 4146.583 kb on + strand at 4146.583 kb on + strand at 4146.583 kb on + strand at 4146.604 kb on + strand at 4146.604 kb on + strand at 4146.605 kb on - strand at 4146.658 kb on - strand at 4146.658 kb on - strand at 4146.660 kb on + strand at 4146.661 kb on - strand at 4146.683 kb on - strand at 4146.721 kb on - strand at 4146.737 kb on + strand at 4146.737 kb on + strand at 4146.756 kb on - strand at 4146.862 kb on + strand at 4146.865 kb on - strand at 4147.139 kb on + strand, within greA at 4147.139 kb on + strand, within greA at 4147.182 kb on + strand, within greA at 4147.245 kb on - strand, within greA at 4147.325 kb on - strand at 4147.328 kb on + strand at 4147.328 kb on + strand at 4147.329 kb on - strand at 4147.338 kb on + strand at 4147.339 kb on - strand at 4147.339 kb on - strand at 4147.339 kb on - strand at 4147.345 kb on - strand at 4147.352 kb on - strand at 4147.376 kb on - strand at 4147.414 kb on - strand at 4147.414 kb on - strand at 4147.492 kb on + strand at 4147.504 kb on - strand at 4147.522 kb on + strand at 4147.523 kb on - strand at 4147.531 kb on + strand at 4147.532 kb on - strand, within yhbY at 4147.576 kb on + strand, within yhbY at 4147.576 kb on + strand, within yhbY at 4147.600 kb on + strand, within yhbY at 4147.601 kb on - strand, within yhbY at 4147.621 kb on + strand, within yhbY at 4147.621 kb on + strand, within yhbY at 4147.783 kb on + strand at 4147.784 kb on - strand at 4147.809 kb on + strand at 4147.851 kb on + strand at 4147.851 kb on + strand at 4147.853 kb on + strand at 4147.853 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 4,145,977 - dacB JDDGAC_21665 0.55 -0.6 4,146,011 + dacB JDDGAC_21665 0.57 +1.8 4,146,039 + dacB JDDGAC_21665 0.59 -1.6 4,146,039 + dacB JDDGAC_21665 0.59 -0.2 4,146,040 - dacB JDDGAC_21665 0.59 -0.3 4,146,040 - dacB JDDGAC_21665 0.59 +0.8 4,146,053 + dacB JDDGAC_21665 0.60 +0.5 4,146,054 - dacB JDDGAC_21665 0.60 -1.7 4,146,054 - dacB JDDGAC_21665 0.60 +0.6 4,146,055 + dacB JDDGAC_21665 0.60 +0.1 4,146,088 - dacB JDDGAC_21665 0.63 +0.1 4,146,119 + dacB JDDGAC_21665 0.65 -0.9 4,146,120 - dacB JDDGAC_21665 0.65 +1.1 4,146,120 - dacB JDDGAC_21665 0.65 -1.2 4,146,120 - dacB JDDGAC_21665 0.65 -0.6 4,146,120 - dacB JDDGAC_21665 0.65 +0.3 4,146,209 - dacB JDDGAC_21665 0.71 +0.8 4,146,209 - dacB JDDGAC_21665 0.71 -0.7 4,146,209 - dacB JDDGAC_21665 0.71 -0.4 4,146,212 + dacB JDDGAC_21665 0.71 -0.3 4,146,213 - dacB JDDGAC_21665 0.71 -0.3 4,146,213 - dacB JDDGAC_21665 0.71 +0.5 4,146,213 - dacB JDDGAC_21665 0.71 +0.7 4,146,213 - dacB JDDGAC_21665 0.71 +0.2 4,146,321 - dacB JDDGAC_21665 0.79 +1.6 4,146,321 - dacB JDDGAC_21665 0.79 +2.0 4,146,331 + dacB JDDGAC_21665 0.80 +0.4 4,146,335 + dacB JDDGAC_21665 0.80 +0.1 4,146,468 + dacB JDDGAC_21665 0.89 -0.0 4,146,469 - dacB JDDGAC_21665 0.89 +0.6 4,146,541 + -0.1 4,146,541 + -0.3 4,146,546 + +0.9 4,146,546 + -0.8 4,146,546 + +0.1 4,146,547 - +0.0 4,146,547 - +0.3 4,146,581 + -2.4 4,146,582 - -0.3 4,146,582 - -1.2 4,146,583 + -0.1 4,146,583 + -0.8 4,146,583 + -1.1 4,146,604 + +1.3 4,146,604 + +1.0 4,146,605 - +0.1 4,146,658 - +0.2 4,146,658 - -0.2 4,146,660 + -1.6 4,146,661 - +0.1 4,146,683 - -0.2 4,146,721 - -0.1 4,146,737 + -0.2 4,146,737 + +0.4 4,146,756 - +0.1 4,146,862 + +0.1 4,146,865 - -1.3 4,147,139 + greA JDDGAC_21670 0.56 -1.9 4,147,139 + greA JDDGAC_21670 0.56 -1.8 4,147,182 + greA JDDGAC_21670 0.65 -0.2 4,147,245 - greA JDDGAC_21670 0.79 -1.0 4,147,325 - -1.2 4,147,328 + -0.7 4,147,328 + -0.3 4,147,329 - +1.1 4,147,338 + +0.8 4,147,339 - -0.4 4,147,339 - -0.2 4,147,339 - +1.1 4,147,345 - -0.9 4,147,352 - -1.0 4,147,376 - -0.2 4,147,414 - -0.1 4,147,414 - +0.3 4,147,492 + -0.6 4,147,504 - -0.3 4,147,522 + +0.3 4,147,523 - +0.0 4,147,531 + -0.5 4,147,532 - yhbY JDDGAC_21675 0.10 -0.7 4,147,576 + yhbY JDDGAC_21675 0.25 -0.6 4,147,576 + yhbY JDDGAC_21675 0.25 -0.6 4,147,600 + yhbY JDDGAC_21675 0.33 -0.7 4,147,601 - yhbY JDDGAC_21675 0.34 -0.5 4,147,621 + yhbY JDDGAC_21675 0.40 +0.4 4,147,621 + yhbY JDDGAC_21675 0.40 +0.0 4,147,783 + -0.3 4,147,784 - +0.5 4,147,809 + -0.5 4,147,851 + -0.2 4,147,851 + +1.3 4,147,853 + -0.1 4,147,853 + +2.0
Or see this region's nucleotide sequence