Experiment: Bas10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagZ and thiK are separated by 10 nucleotides thiK and lpoB overlap by 20 nucleotides lpoB and JDDGAC_07180 are separated by 13 nucleotides JDDGAC_07180 and hinT are separated by 2 nucleotides
JDDGAC_07165: nagZ - beta-N-acetylhexosaminidase, at 1,240,688 to 1,241,713
nagZ
JDDGAC_07170: thiK - thiamine kinase, at 1,241,724 to 1,242,548
thiK
JDDGAC_07175: lpoB - penicillin-binding protein activator LpoB, at 1,242,529 to 1,243,170
lpoB
JDDGAC_07180: JDDGAC_07180 - DUF1425 domain-containing protein, at 1,243,184 to 1,243,561
_07180
JDDGAC_07185: hinT - purine nucleoside phosphoramidase, at 1,243,564 to 1,243,923
hinT
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1241.532 kb on - strand, within nagZ at 1241.607 kb on + strand, within nagZ at 1241.683 kb on - strand at 1241.734 kb on - strand at 1241.737 kb on - strand at 1241.900 kb on - strand, within thiK at 1241.983 kb on + strand, within thiK at 1241.983 kb on + strand, within thiK at 1242.067 kb on - strand, within thiK at 1242.155 kb on - strand, within thiK at 1242.211 kb on - strand, within thiK at 1242.215 kb on - strand, within thiK at 1242.217 kb on + strand, within thiK at 1242.253 kb on + strand, within thiK at 1242.306 kb on + strand, within thiK at 1242.307 kb on - strand, within thiK at 1242.307 kb on - strand, within thiK at 1242.461 kb on + strand, within thiK at 1242.602 kb on - strand, within lpoB at 1242.638 kb on + strand, within lpoB at 1242.648 kb on - strand, within lpoB at 1242.648 kb on - strand, within lpoB at 1242.676 kb on + strand, within lpoB at 1242.835 kb on - strand, within lpoB at 1242.837 kb on + strand, within lpoB at 1242.853 kb on - strand, within lpoB at 1242.904 kb on - strand, within lpoB at 1242.938 kb on + strand, within lpoB at 1243.185 kb on + strand at 1243.185 kb on + strand at 1243.191 kb on + strand at 1243.197 kb on + strand at 1243.321 kb on - strand, within JDDGAC_07180 at 1243.366 kb on + strand, within JDDGAC_07180 at 1243.369 kb on - strand, within JDDGAC_07180 at 1243.369 kb on - strand, within JDDGAC_07180 at 1243.397 kb on - strand, within JDDGAC_07180 at 1243.520 kb on + strand, within JDDGAC_07180 at 1243.576 kb on - strand at 1243.692 kb on + strand, within hinT at 1243.909 kb on - strand at 1243.985 kb on + strand at 1244.024 kb on - strand at 1244.064 kb on - strand at 1244.065 kb on + strand at 1244.066 kb on - strand at 1244.066 kb on - strand at 1244.068 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas10 remove 1,241,532 - nagZ JDDGAC_07165 0.82 -0.0 1,241,607 + nagZ JDDGAC_07165 0.90 +0.5 1,241,683 - -1.3 1,241,734 - +0.4 1,241,737 - +1.3 1,241,900 - thiK JDDGAC_07170 0.21 +0.0 1,241,983 + thiK JDDGAC_07170 0.31 +2.3 1,241,983 + thiK JDDGAC_07170 0.31 +0.6 1,242,067 - thiK JDDGAC_07170 0.42 -0.7 1,242,155 - thiK JDDGAC_07170 0.52 +0.1 1,242,211 - thiK JDDGAC_07170 0.59 -0.3 1,242,215 - thiK JDDGAC_07170 0.60 +0.2 1,242,217 + thiK JDDGAC_07170 0.60 -0.6 1,242,253 + thiK JDDGAC_07170 0.64 +0.3 1,242,306 + thiK JDDGAC_07170 0.71 +0.6 1,242,307 - thiK JDDGAC_07170 0.71 -0.7 1,242,307 - thiK JDDGAC_07170 0.71 -0.9 1,242,461 + thiK JDDGAC_07170 0.89 -0.8 1,242,602 - lpoB JDDGAC_07175 0.11 -0.4 1,242,638 + lpoB JDDGAC_07175 0.17 -0.1 1,242,648 - lpoB JDDGAC_07175 0.19 -0.8 1,242,648 - lpoB JDDGAC_07175 0.19 -1.5 1,242,676 + lpoB JDDGAC_07175 0.23 -1.8 1,242,835 - lpoB JDDGAC_07175 0.48 -0.9 1,242,837 + lpoB JDDGAC_07175 0.48 -0.9 1,242,853 - lpoB JDDGAC_07175 0.50 -0.6 1,242,904 - lpoB JDDGAC_07175 0.58 +1.6 1,242,938 + lpoB JDDGAC_07175 0.64 -0.3 1,243,185 + -0.8 1,243,185 + +0.6 1,243,191 + -2.1 1,243,197 + -0.3 1,243,321 - JDDGAC_07180 0.36 -1.3 1,243,366 + JDDGAC_07180 0.48 -0.0 1,243,369 - JDDGAC_07180 0.49 +0.0 1,243,369 - JDDGAC_07180 0.49 +0.0 1,243,397 - JDDGAC_07180 0.56 +0.8 1,243,520 + JDDGAC_07180 0.89 +1.3 1,243,576 - +0.0 1,243,692 + hinT JDDGAC_07185 0.36 -0.2 1,243,909 - +0.7 1,243,985 + +1.1 1,244,024 - -0.2 1,244,064 - +0.8 1,244,065 + -0.8 1,244,066 - +0.2 1,244,066 - -1.0 1,244,068 - +0.2
Or see this region's nucleotide sequence