Strain Fitness in Escherichia coli ECRC98 around JDDGAC_07175

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnagZ and thiK are separated by 10 nucleotidesthiK and lpoB overlap by 20 nucleotideslpoB and JDDGAC_07180 are separated by 13 nucleotidesJDDGAC_07180 and hinT are separated by 2 nucleotides JDDGAC_07165: nagZ - beta-N-acetylhexosaminidase, at 1,240,688 to 1,241,713 nagZ JDDGAC_07170: thiK - thiamine kinase, at 1,241,724 to 1,242,548 thiK JDDGAC_07175: lpoB - penicillin-binding protein activator LpoB, at 1,242,529 to 1,243,170 lpoB JDDGAC_07180: JDDGAC_07180 - DUF1425 domain-containing protein, at 1,243,184 to 1,243,561 _07180 JDDGAC_07185: hinT - purine nucleoside phosphoramidase, at 1,243,564 to 1,243,923 hinT Position (kb) 1242 1243 1244Strain fitness (log2 ratio) -2 -1 0 1 2at 1241.532 kb on - strand, within nagZat 1241.607 kb on + strand, within nagZat 1241.683 kb on - strandat 1241.734 kb on - strandat 1241.737 kb on - strandat 1241.900 kb on - strand, within thiKat 1241.983 kb on + strand, within thiKat 1241.983 kb on + strand, within thiKat 1242.067 kb on - strand, within thiKat 1242.155 kb on - strand, within thiKat 1242.211 kb on - strand, within thiKat 1242.215 kb on - strand, within thiKat 1242.217 kb on + strand, within thiKat 1242.253 kb on + strand, within thiKat 1242.306 kb on + strand, within thiKat 1242.307 kb on - strand, within thiKat 1242.307 kb on - strand, within thiKat 1242.461 kb on + strand, within thiKat 1242.602 kb on - strand, within lpoBat 1242.638 kb on + strand, within lpoBat 1242.648 kb on - strand, within lpoBat 1242.648 kb on - strand, within lpoBat 1242.676 kb on + strand, within lpoBat 1242.835 kb on - strand, within lpoBat 1242.837 kb on + strand, within lpoBat 1242.853 kb on - strand, within lpoBat 1242.904 kb on - strand, within lpoBat 1242.938 kb on + strand, within lpoBat 1243.185 kb on + strandat 1243.185 kb on + strandat 1243.191 kb on + strandat 1243.197 kb on + strandat 1243.321 kb on - strand, within JDDGAC_07180at 1243.366 kb on + strand, within JDDGAC_07180at 1243.369 kb on - strand, within JDDGAC_07180at 1243.369 kb on - strand, within JDDGAC_07180at 1243.397 kb on - strand, within JDDGAC_07180at 1243.520 kb on + strand, within JDDGAC_07180at 1243.576 kb on - strandat 1243.692 kb on + strand, within hinTat 1243.909 kb on - strandat 1243.985 kb on + strandat 1244.024 kb on - strandat 1244.064 kb on - strandat 1244.065 kb on + strandat 1244.066 kb on - strandat 1244.066 kb on - strandat 1244.068 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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1,241,532 - nagZ JDDGAC_07165 0.82 -0.0
1,241,607 + nagZ JDDGAC_07165 0.90 +0.5
1,241,683 - -1.3
1,241,734 - +0.4
1,241,737 - +1.3
1,241,900 - thiK JDDGAC_07170 0.21 +0.0
1,241,983 + thiK JDDGAC_07170 0.31 +2.3
1,241,983 + thiK JDDGAC_07170 0.31 +0.6
1,242,067 - thiK JDDGAC_07170 0.42 -0.7
1,242,155 - thiK JDDGAC_07170 0.52 +0.1
1,242,211 - thiK JDDGAC_07170 0.59 -0.3
1,242,215 - thiK JDDGAC_07170 0.60 +0.2
1,242,217 + thiK JDDGAC_07170 0.60 -0.6
1,242,253 + thiK JDDGAC_07170 0.64 +0.3
1,242,306 + thiK JDDGAC_07170 0.71 +0.6
1,242,307 - thiK JDDGAC_07170 0.71 -0.7
1,242,307 - thiK JDDGAC_07170 0.71 -0.9
1,242,461 + thiK JDDGAC_07170 0.89 -0.8
1,242,602 - lpoB JDDGAC_07175 0.11 -0.4
1,242,638 + lpoB JDDGAC_07175 0.17 -0.1
1,242,648 - lpoB JDDGAC_07175 0.19 -0.8
1,242,648 - lpoB JDDGAC_07175 0.19 -1.5
1,242,676 + lpoB JDDGAC_07175 0.23 -1.8
1,242,835 - lpoB JDDGAC_07175 0.48 -0.9
1,242,837 + lpoB JDDGAC_07175 0.48 -0.9
1,242,853 - lpoB JDDGAC_07175 0.50 -0.6
1,242,904 - lpoB JDDGAC_07175 0.58 +1.6
1,242,938 + lpoB JDDGAC_07175 0.64 -0.3
1,243,185 + -0.8
1,243,185 + +0.6
1,243,191 + -2.1
1,243,197 + -0.3
1,243,321 - JDDGAC_07180 0.36 -1.3
1,243,366 + JDDGAC_07180 0.48 -0.0
1,243,369 - JDDGAC_07180 0.49 +0.0
1,243,369 - JDDGAC_07180 0.49 +0.0
1,243,397 - JDDGAC_07180 0.56 +0.8
1,243,520 + JDDGAC_07180 0.89 +1.3
1,243,576 - +0.0
1,243,692 + hinT JDDGAC_07185 0.36 -0.2
1,243,909 - +0.7
1,243,985 + +1.1
1,244,024 - -0.2
1,244,064 - +0.8
1,244,065 + -0.8
1,244,066 - +0.2
1,244,066 - -1.0
1,244,068 - +0.2

Or see this region's nucleotide sequence