Strain Fitness in Escherichia coli ECRC98 around JDDGAC_06125

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsirB2 and prmC overlap by 4 nucleotidesprmC and prfA overlap by 1 nucleotidesprfA and hemA are separated by 41 nucleotides JDDGAC_06115: sirB2 - invasion regulator SirB2, at 1,064,350 to 1,064,742 sirB2 JDDGAC_06120: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 1,064,739 to 1,065,572 prmC JDDGAC_06125: prfA - peptide chain release factor 1, at 1,065,572 to 1,066,654 prfA JDDGAC_06130: hemA - glutamyl-tRNA reductase, at 1,066,696 to 1,067,952 hemA Position (kb) 1065 1066 1067Strain fitness (log2 ratio) -1 0 1 2 3at 1064.581 kb on + strand, within sirB2at 1064.703 kb on + strandat 1064.704 kb on - strandat 1064.706 kb on + strandat 1065.015 kb on - strand, within prmCat 1065.400 kb on - strand, within prmCat 1065.553 kb on - strandat 1066.052 kb on - strand, within prfAat 1066.141 kb on - strand, within prfAat 1066.692 kb on - strandat 1066.692 kb on - strandat 1066.692 kb on - strandat 1066.692 kb on - strandat 1067.011 kb on - strand, within hemA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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1,064,581 + sirB2 JDDGAC_06115 0.59 +1.6
1,064,703 + -0.7
1,064,704 - +0.5
1,064,706 + +0.6
1,065,015 - prmC JDDGAC_06120 0.33 +2.9
1,065,400 - prmC JDDGAC_06120 0.79 -0.3
1,065,553 - -0.0
1,066,052 - prfA JDDGAC_06125 0.44 -1.5
1,066,141 - prfA JDDGAC_06125 0.53 -1.0
1,066,692 - -1.5
1,066,692 - +2.9
1,066,692 - -0.3
1,066,692 - -1.2
1,067,011 - hemA JDDGAC_06130 0.25 +0.3

Or see this region's nucleotide sequence