Strain Fitness in Escherichia coli ECRC98 around JDDGAC_04595

Experiment: Bas10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_04575 and JDDGAC_04580 are separated by 275 nucleotidesJDDGAC_04580 and JDDGAC_04590 are separated by 188 nucleotidesJDDGAC_04590 and JDDGAC_04595 are separated by 115 nucleotidesJDDGAC_04595 and JDDGAC_04600 overlap by 4 nucleotidesJDDGAC_04600 and JDDGAC_04605 are separated by 117 nucleotidesJDDGAC_04605 and JDDGAC_04610 are separated by 48 nucleotides JDDGAC_04575: JDDGAC_04575 - Bacteriophage cohesive ends, at 802,293 to 802,505 _04575 JDDGAC_04580: JDDGAC_04580 - Hok/gef family, at 802,781 to 802,993 _04580 JDDGAC_04590: JDDGAC_04590 - hypothetical protein, at 803,182 to 803,286 _04590 JDDGAC_04595: JDDGAC_04595 - DUF551 domain-containing protein, at 803,402 to 803,755 _04595 JDDGAC_04600: JDDGAC_04600 - hypothetical protein, at 803,752 to 804,042 _04600 JDDGAC_04605: JDDGAC_04605 - hypothetical protein, at 804,160 to 804,372 _04605 JDDGAC_04610: JDDGAC_04610 - Ead/Ea22-like protein, at 804,421 to 804,777 _04610 Position (kb) 803 804Strain fitness (log2 ratio) -1 0 1at 802.439 kb on + strand, within JDDGAC_04575at 802.527 kb on - strandat 802.644 kb on + strandat 803.349 kb on + strandat 803.503 kb on + strand, within JDDGAC_04595at 803.507 kb on - strand, within JDDGAC_04595at 803.558 kb on + strand, within JDDGAC_04595at 804.032 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas10
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802,439 + JDDGAC_04575 0.69 +1.4
802,527 - -0.7
802,644 + -1.1
803,349 + -0.3
803,503 + JDDGAC_04595 0.29 -1.0
803,507 - JDDGAC_04595 0.30 -0.9
803,558 + JDDGAC_04595 0.44 +0.2
804,032 - +0.3

Or see this region's nucleotide sequence